This study aims to validate CO dispersion model due to the position of the road toward the dominant wind direction on the transport sector. Sampling for modelling was done on the road with the road angle to wind direction is 0 degree (Jend. A. Yani Road), 30 degree (Andalas Road) and 60 degree (Prof. Dr. Hamka Road). CO dispersion model was obtained from the relations between CO concentration with traffic volume, traffic speed, wind speed and dominant wind direction. Sampling for validation was done at three location points, i.e. Jend. Ahmad Yani Road, By Pass Road and Dr. Wahidin Road, each of which has a position of 0, 45 and 90 degrees toward dominant wind direction. Sampling for CO was done using impinger. Measurement of traffic characteristics and meteorological conditions was performed in conjunction with CO sampling. Validation test was done by using Pearson Product Moment formula and Test of Two Variance. Results of the Two-Variance Test showed no significant difference between two concentrations of CO model and CO measurement. It showed the Test Ratio (RUf) smaller than the Critical Point. Validation test using Pearson Product Moment showed that the CO model can be used for predicting CO dispersion.