<i><scp>T</scp>rypanosoma brucei gambiense</i> infection in vervet monkeys: a potential model for early‐stage disease

Gachie, Beatrice; Kagira, John; Karanja, Simon; Waema, Maxwell W.; Ngotho, J. M.; Maina, Naomi

doi:10.1111/jmp.12100

Cited by 3 publications

(2 citation statements)

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“…The higher sensitivity of LAMP compared to parasitological methods and PCR is also of significance in regard to the search for new diagnostic tests for sleeping sickness. In the current study the performance of LAMP and PCR was assessed in an early stage HAT model [ 15 ].…”

Section: Discussionmentioning

confidence: 99%

“…The importance of experimental animal models includes controlled conditions and planned sampling among other. The vervet monkey ( Chlorocebus aethiops ) has been developed as a model for early stage HAT caused by T. b. gambiense [ 15 ]. Using this animal model, the performance of LAMP based on the TgsGP gene was assessed in detection of T. b gambiense in serum, CSF, saliva, and urine.…”

Section: Introductionmentioning

confidence: 99%

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Loop Mediated Isothermal Amplification for Detection ofTrypanosoma brucei gambiensein Urine and Saliva Samples in Nonhuman Primate Model

Ngotho

Kagira

Gachie

et al. 2015

BioMed Research International

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Human African trypanosomiasis (HAT) is a vector-borne parasitic zoonotic disease. The disease caused by Trypanosoma brucei gambiense is the most prevalent in Africa. Early diagnosis is hampered by lack of sensitive diagnostic techniques. This study explored the potential of loop mediated isothermal amplification (LAMP) and polymerase chain reaction (PCR) in the detection of T. b. gambiense infection in a vervet monkey HAT model. Six vervet monkeys were experimentally infected with T. b. gambiense IL3253 and monitored for 180 days after infection. Parasitaemia was scored daily. Blood, cerebrospinal fluid (CSF), saliva, and urine samples were collected weekly. PCR and LAMP were performed on serum, CSF, saliva, and urine samples. The detection by LAMP was significantly higher than that of parasitological methods and PCR in all the samples. The performance of LAMP varied between the samples and was better in serum followed by saliva and then urine samples. In the saliva samples, LAMP had 100% detection between 21 and 77 dpi, whereas in urine the detection it was slightly lower, but there was over 80% detection between 28 and 91 dpi. However, LAMP could not detect trypanosomes in either saliva or urine after 140 and 126 dpi, respectively. The findings of this study emphasize the importance of LAMP in diagnosis of HAT using saliva and urine samples.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Loop Mediated Isothermal Amplification for Detection ofTrypanosoma brucei gambiensein Urine and Saliva Samples in Nonhuman Primate Model

Ngotho

Kagira

Gachie

et al. 2015

BioMed Research International

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Animal Models of Infectious Diseases

Jena,

Pawar

2024

Animal Models in Research

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Age and sex associated organ weight differences in vervets/African green monkeys (Chlorocebus aethiops sabaeus)

Balamayooran,

Tooze,

Gardin

et al. 2024

J of Medical Primatology

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BackgroundAfrican green monkeys (AGMs, also known as vervets, Cholorocebus aethiops sabaeus) have been used in a variety of biomedical research studies. The aim of this study was to generate a reference for normal organ weights and percentage organ weights in AGMs of different age categories and sex.MethodsThe organ weights were compiled from 479 AGMs (285 females and 194 males) from 2004 to 2021. Age and sex differences of absolute and relative organ weights were analyzed using analysis of variance.ResultsThe findings demonstrate that males had higher body and organ weights than age‐matched females, but relative organ weights did not differ between males and females. At maturity, adrenal gland, brain, kidney, liver, thymus, and thyroid gland weights as a percentage of body weight declined, but relative weights of prostate gland, testes, and uterus were higher.ConclusionThese data should be beneficial to biomedical researchers and pathologists working with AGMs.

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Trypanosoma brucei gambiense infection in vervet monkeys: a potential model for early‐stage disease

Abstract: This vervet monkey model can only be used for early-stage disease Gambian sleeping sickness.

Cited by 3 publications

References 23 publications

Loop Mediated Isothermal Amplification for Detection ofTrypanosoma brucei gambiensein Urine and Saliva Samples in Nonhuman Primate Model

Loop Mediated Isothermal Amplification for Detection ofTrypanosoma brucei gambiensein Urine and Saliva Samples in Nonhuman Primate Model

Animal Models of Infectious Diseases

Age and sex associated organ weight differences in vervets/African green monkeys (Chlorocebus aethiops sabaeus)

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