<i><scp>V</scp>ibrio parahaemolyticus</i> type <scp>IV</scp> pili mediate interactions with diatom‐derived chitin and point to an unexplored mechanism of environmental persistence

Frischkorn, Kyle R.; Stojanovski, Asta; Paranjpye, Rohinee N.

doi:10.1111/1462-2920.12093

Cited by 66 publications

(51 citation statements)

References 53 publications

(97 reference statements)

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“…Type IV pilins, like the one detected in Synechococcus sp. WH7803, have also been reported to have a potential role in chitin adhesion in Vibrio (Frischkorn et al ., 2013). Hence, picocyanobacteria may appear to be more hostile microorganisms than previously anticipated.…”

Section: Discussionmentioning

confidence: 99%

Functional distinctness in the exoproteomes of marine Synechococcus

Christie-Oleza

Armengaud

Guérin

et al. 2015

Environmental Microbiology

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SummaryThe exported protein fraction of an organism may reflect its life strategy and, ultimately, the way it is perceived by the outside world. Bioinformatic prediction of the exported pan‐proteome of P rochlorococcus and S ynechococcus lineages demonstrated that (i) this fraction of the encoded proteome had a much higher incidence of lineage‐specific proteins than the cytosolic fraction (57% and 73% homologue incidence respectively) and (ii) exported proteins are largely uncharacterized to date (54%) compared with proteins from the cytosolic fraction (35%). This suggests that the genomic and functional diversity of these organisms lies largely in the diverse pool of novel functions these organisms export to/through their membranes playing a key role in community diversification, e.g. for niche partitioning or evading predation. Experimental exoproteome analysis of marine S ynechococcus showed transport systems for inorganic nutrients, an interesting array of strain‐specific exoproteins involved in mutualistic or hostile interactions (i.e. hemolysins, pilins, adhesins), and exoenzymes with a potential mixotrophic goal (i.e. exoproteases and chitinases). We also show how these organisms can remodel their exoproteome, i.e. by increasing the repertoire of interaction proteins when grown in the presence of a heterotroph or decrease exposure to prey when grown in the dark. Finally, our data indicate that heterotrophic bacteria can feed on the exoproteome of S ynechococcus.

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Section: Discussionmentioning

confidence: 99%

Functional distinctness in the exoproteomes of marine Synechococcus

Christie-Oleza

Armengaud

Guérin

et al. 2015

Environmental Microbiology

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“…Other exudates may be involved in species-specific interactions. Chitin, produced as a component of the diatom frustule in some species (64), has been shown to stimulate expression of functional type IV pili in V. parahaemolyticus, resulting in an increase in adherence (64 (66). In another study, Kim et al (67) demonstrated that the raphidophyte Olisthodiscus luteus (later changed to Heterosigma akashiwo) inhibited the growth of V. alginolyticus by reactive oxygen species-mediated processes.…”

Section: Discussionmentioning

confidence: 99%

Community-Level and Species-Specific Associations between Phytoplankton and Particle-Associated Vibrio Species in Delaware's Inland Bays

Main

Salvitti

Whereat

et al. 2015

Appl Environ Microbiol

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Vibrio species are an abundant and diverse group of bacteria that form associations with phytoplankton. Correlations between Vibrio and phytoplankton abundance have been noted, suggesting that growth is enhanced during algal blooms or that association with phytoplankton provides a refuge from predation. Here, we investigated relationships between particle-associated Vibrio spp. and phytoplankton in Delaware's inland bays (DIB). The relative abundances of particle-associated Vibrio spp. and algal classes that form blooms in DIB (dinoflagellates, diatoms, and raphidophytes) were determined using quantitative PCR. The results demonstrated a significant correlation between particle-associated Vibrio abundance and phytoplankton, with higher correlations to diatoms and raphidophytes than to dinoflagellates. Species-specific associations were examined during a mixed bloom of Heterosigma akashiwo and Fibrocapsa japonica (Raphidophyceae) and indicated a significant positive correlation for particle-associated Vibrio abundance with H. akashiwo but a negative correlation with F. japonica. Changes in Vibrio assemblages during the bloom were evaluated using automated ribosomal intergenic spacer analysis (ARISA), which revealed significant differences between each size fraction but no significant change in Vibrio assemblages over the course of the bloom. Microzooplankton grazing experiments showed that losses of particle-associated Vibrio spp. may be offset by increased growth in the Vibrio population. Moreover, analysis of Vibrio assemblages by ARISA also indicated an increase in the relative abundance for specific members of the Vibrio community despite higher grazing pressure on the particle-associated population as a whole. The results of this investigation demonstrate links between phytoplankton and Vibrio that may lead to predictions of potential health risks and inform future management practices in this region. Bacteria within the genus Vibrio are naturally abundant in marine and estuarine environments (1), where they exhibit two alternative growth strategies: (i) association with particles as a biofilm or (ii) as free-living bacterioplankton (2). Association with planktonic organisms plays an important role in the ecology of Vibrio (3) by providing an enriched microenvironment for Vibrio spp. (4-6). Previous research demonstrated that planktonic copepods, in particular, enhance the survival and distribution of Vibrio in temperate and tropical areas (7,8). Associations with plankton may also provide a refuge from grazing by bacterivorous protozoa (9, 10), which can be substantial in some areas (11). Matz et al. (9), for example, showed that the cell density of Vibrio cholerae within a biofilm remained stable in the presence of protozoa, whereas planktonic cells were rapidly eliminated. However, particle association may not always be advantageous, as it may subject the cells to sinking forces or losses through "collateral damage" when host cells are preyed upon (10).In addition to copepods, Vibrio spp. also form attach...

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“…The utilization of chitin by vibrios involves multiple levels of gene regulation that govern motility, chemotaxis, extracellular polysaccharide and biofilm matrix protein synthesis and secretion, type IV pilus production, chitin-binding protein secretion, chitin surface attachment, biofilm formation, extracellular chitinase secretion, chitoporin expression, and competence (166,338,470,(520)(521)(522)(523). Association with insoluble materials may be the preferred lifestyle of vibrios, including deep-sea hydrothermal vent species (15,403,524), and regulatory systems involving TCSs, chemotaxis, QS, sRNAs, cAMP, c-di-GMP, alternative sigma factors, and the stringent response enable vibrios to optimize resource utilization and survival (Fig.…”

Section: An Example Of Microbial Interaction With Surfaces: Vibrio Chmentioning

confidence: 99%

Microbial Surface Colonization and Biofilm Development in Marine Environments

Dang

Lovell

2016

Microbiol Mol Biol Rev

870

636

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SUMMARYBiotic and abiotic surfaces in marine waters are rapidly colonized by microorganisms. Surface colonization and subsequent biofilm formation and development provide numerous advantages to these organisms and support critical ecological and biogeochemical functions in the changing marine environment. Microbial surface association also contributes to deleterious effects such as biofouling, biocorrosion, and the persistence and transmission of harmful or pathogenic microorganisms and their genetic determinants. The processes and mechanisms of colonization as well as key players among the surface-associated microbiota have been studied for several decades. Accumulating evidence indicates that specific cell-surface, cell-cell, and interpopulation interactions shape the composition, structure, spatiotemporal dynamics, and functions of surface-associated microbial communities. Several key microbial processes and mechanisms, including (i) surface, population, and community sensing and signaling, (ii) intraspecies and interspecies communication and interaction, and (iii) the regulatory balance between cooperation and competition, have been identified as critical for the microbial surface association lifestyle. In this review, recent progress in the study of marine microbial surface colonization and biofilm development is synthesized and discussed. Major gaps in our knowledge remain. We pose questions for targeted investigation of surface-specific community-level microbial features, answers to which would advance our understanding of surface-associated microbial community ecology and the biogeochemical functions of these communities at levels from molecular mechanistic details through systems biological integration.

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Vibrio parahaemolyticus type IV pili mediate interactions with diatom‐derived chitin and point to an unexplored mechanism of environmental persistence

Cited by 66 publications

References 53 publications

Functional distinctness in the exoproteomes of marine Synechococcus

Functional distinctness in the exoproteomes of marine Synechococcus

Community-Level and Species-Specific Associations between Phytoplankton and Particle-Associated Vibrio Species in Delaware's Inland Bays

Microbial Surface Colonization and Biofilm Development in Marine Environments

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