<scp>Z</scp>inc <scp>F</scp>inger <scp>P</scp>rotein 6 (<scp>ZFP</scp>6) regulates trichome initiation by integrating gibberellin and cytokinin signaling in <scp>A</scp>rabidopsis thaliana

Zhou, Zhongjing; Sun, Lili; Zhao, Yongqin; An, Lijun; An, Yan; Meng, Xiaofang; Gan, Yinbo

doi:10.1111/nph.12211

Cited by 157 publications

(159 citation statements)

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“…ZFP2 was shown to control floral abscission, as overexpressing plants enhanced the retention of floral organs (Cai and Lashbrook, 2008). ZFP5, ZFP6, and ZFP8 were shown to regulate the differentiation of trichomes through integrating GA and cytokinin signaling (Zhou et al, 2011(Zhou et al, , 2013. We did not observe abnormalities in the trichome morphology of ZFP3ox or zfp3 mutant seedlings (data not shown).…”

Section: Discussionmentioning

confidence: 65%

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

et al. 2014

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Seed germination is controlled by environmental signals, including light and endogenous phytohormones. Abscisic acid (ABA) inhibits, whereas gibberellin promotes, germination and early seedling development, respectively. Here, we report that ZFP3, a nuclear C2H2 zinc finger protein, acts as a negative regulator of ABA suppression of seed germination in Arabidopsis (Arabidopsis thaliana). Accordingly, regulated overexpression of ZFP3 and the closely related ZFP1, ZFP4, ZFP6, and ZFP7 zinc finger factors confers ABA insensitivity to seed germination, while the zfp3 zfp4 double mutant displays enhanced ABA susceptibility. Reduced expression of several ABA-induced genes, such as RESPONSIVE TO ABSCISIC ACID18 and transcription factor ABSCISIC ACID-INSENSITIVE4 (ABI4), in ZFP3 overexpression seedlings suggests that ZFP3 negatively regulates ABA signaling. Analysis of ZFP3 overexpression plants revealed multiple phenotypic alterations, such as semidwarf growth habit, defects in fertility, and enhanced sensitivity of hypocotyl elongation to red but not to far-red or blue light. Analysis of genetic interactions with phytochrome and abi mutants indicates that ZFP3 enhances red light signaling by photoreceptors other than phytochrome A and additively increases ABA insensitivity conferred by the abi2, abi4, and abi5 mutations. These data support the conclusion that ZFP3 and the related ZFP subfamily of zinc finger factors regulate light and ABA responses during germination and early seedling development.

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Section: Discussionmentioning

confidence: 65%

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

et al. 2014

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“…After leaf development, ammonium liberated from protein turnover and amino acid catabolism is assimilated into Gln, which is translocated in the vascular bundles to new sinks (Zhang et al, 2010;Pratelli et al, 2012). Among the new sinks are trichomes that are the first cells forming during new leaf development (Zhou et al, 2013). Our amino acid results showed a significant decrease in Gln content in the Gln-1;2 knock-out mutants (Fig.…”

Section: Expression Of Gs1 Isoforms In Arabidopsis Shoots In Relationmentioning

confidence: 62%

Cytosolic Glutamine Synthetase Gln1;2 Is the Main Isozyme Contributing to GS1 Activity and Can Be Up-Regulated to Relieve Ammonium Toxicity

et al. 2016

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Cytosolic GS1 (Gln synthetase) is central for ammonium assimilation in plants. High ammonium treatment enhanced the expression of the GS1 isogene Gln-1;2 encoding a low-affinity high-capacity GS1 protein in Arabidopsis (Arabidopsis thaliana) shoots. Under the same conditions, the expression of the high-affinity low-capacity isoform Gln-1;1 was reduced. The expression of Gln-1;3 did not respond to ammonium treatment while Gln-1;4 and Gln-1;5 isogenes in all cases were expressed at a very low level. Gln-2 was highly expressed in shoots but only at a very low level in roots. To investigate the specific functions of the two isogenes Gln-1;1 and Gln-1;2 in shoots for ammonium detoxification, single and double knock-out mutants were grown under standard N supply or with high ammonium provision. Phenotypes of the single mutant gln1;1 were similar to the wild type, while growth of the gln1;2 single mutant and the gln1;1:gln1;2 double mutant was significantly impaired irrespective of N regime. GS1 activity was significantly reduced in both gln1;2 and gln1;1:gln1;2. Along with this, the ammonium content increased while that of Gln decreased, showing that Gln-1;2 was essential for ammonium assimilation and amino acid synthesis. We conclude that Gln-1;2 is the main isozyme contributing to shoot GS1 activity in vegetative growth stages and can be up-regulated to relieve ammonium toxicity. This reveals, to our knowledge, a novel shoot function of Gln-1;2 in Arabidopsis shoots.

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“…Data represent the mean 6 SD of 18 plants. Yu et al, 2010;Zhou et al, 2011Zhou et al, , 2013Sun et al, 2015). To examine if this is the case for NTL8, we tested, by using qRT-PCR, the expression of the MBW complex component genes TTG1, GL1, GL3, and EGL3, and their target genes GL2 and singlerepeat R3 MYB genes TRY, CPC, ETC1, ETC2, ETC3, TCL1, and TCL2 in the ntl8-1D mutant seedlings.…”

Section: Trichome Formation In the Ntl8 Mutants Is Largely Unaffectedmentioning

confidence: 99%

“…The C2H2 transcription factors GLABROUS INFLORES-CENCE STEMS (GIS), GIS2, and GIS3, the ZINC FINGER transcription factors ZINC FINGER PROTEIN5 (ZFP5), ZFP6, and ZFP8, and the plant-specific transcription factor SQUAMOSA PROMOTER BINDING PROTEIN LIKE9 (SPL9) have been identified as regulators of trichome formation (Gan et al, 2006(Gan et al, , 2007Yu et al, 2010;Zhou et al, 2011Zhou et al, , 2013Sun et al, 2015). Most of these transcription factors regulate trichome formation via regulating, directly or indirectly, the expression of the key transcription factor genes.…”

mentioning

confidence: 99%

“…Most of these transcription factors regulate trichome formation via regulating, directly or indirectly, the expression of the key transcription factor genes. For example, GIS, GIS3, ZFP5, and ZFP8 can regulate the expression of the MBW complex component genes (Gan et al, 2006(Gan et al, , 2007Zhou et al, 2011Zhou et al, , 2013Sun et al, 2015), whereas SPL9 can directly regulate the expression of the R3 MYB transcription factor genes TRY and TCL1 (Yu et al, 2010).…”

mentioning

confidence: 99%

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NTL8 Regulates Trichome Formation in Arabidopsis by Directly Activating R3 MYB Genes TRY and TCL1

Tian

Guo

et al. 2017

Plant Physiol.

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The NAM, ATAF1/2, and CUC (NAC) are plant-specific transcription factors that regulate multiple aspects of plant growth and development and plant response to environmental stimuli. We report here the identification of NTM1-LIKE8 (NTL8), a membrane-associated NAC transcription factor, as a novel regulator of trichome formation in Arabidopsis (Arabidopsis thaliana). From an activation-tagged Arabidopsis population, we identified a dominant, gain-of-function mutant with glabrous inflorescence stem. By using plasmid rescue and RT-PCR analyses, we found that NTL8 was tagged; thus, the mutant was named ntl8-1 Dominant (ntl8-1D). Recapitulation experiment further confirmed that the phenotype observed in the ntl8-1D mutant was caused by elevated expression of NTL8. Quantitative RT-PCR results showed that the expression level of the single-repeat R3 MYB genes TRIPTYCHON (TRY) and TRICHOMELESS1 (TCL1) was elevated in the ntl8-1D mutant. Genetic analyses demonstrated that NTL8 acts upstream of TRY and TCL1 in the regulation of trichome formation. When recruited to the promoter region of the reporter gene Gal4:GUS by a fused GAL4 DNA-binding domain, NTL8 activated the expression of the reporter gene. Chromatin immunoprecipitation results indicated that TRY and TCL1 are direct targets of NTL8. However, NTL8 did not interact with SQUAMOSA PROMOTER BINDING PROTEIN LIKE9, another transcription factor that regulates the expression of TRY and TCL1, in yeast and plant cells. Taken together, our results suggest that NTL8 negatively regulates trichome formation in Arabidopsis by directly activating the expression of TRY and TCL1.

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Zinc Finger Protein 6 (ZFP6) regulates trichome initiation by integrating gibberellin and cytokinin signaling in Arabidopsis thaliana

Cited by 157 publications

References 47 publications

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

The Arabidopsis ZINC FINGER PROTEIN3 Interferes with Abscisic Acid and Light Signaling in Seed Germination and Plant Development

Cytosolic Glutamine Synthetase Gln1;2 Is the Main Isozyme Contributing to GS1 Activity and Can Be Up-Regulated to Relieve Ammonium Toxicity

NTL8 Regulates Trichome Formation in Arabidopsis by Directly Activating R3 MYB Genes TRY and TCL1

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