<i>SECTION OF BIOLOGY</i>: THE USE OF NEOTETRAZOLIUM AS A TOOL IN THE STUDY OF ACTIVE CELL PROCESSES*

Antopol, William; Glaubach, Susi; Goldman, Lester M.

doi:10.1111/j.2164-0947.1950.tb01889.x

Cited by 22 publications

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“…In addition, one can observe these two structures as discrete entities in uninfected and infected cells by staining differentially first with 2,3,5-triphenyltetrazolium chloride and then by subsequent acid hydrolysis and nuclear staining. That there is no nuclear staining in higher organisms with the tetrazoles has been reported by Zweifach et al (1950), Antopol et al (1950), and Rutenburg et al (1950). Our experience with E. coli has been the same.…”

Section: Discussionsupporting

confidence: 59%

Light and Electron Microscopic Studies of Escherichia Coli-Coliphage Interactions Iii

et al. 1953

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The first two articles of this series (Beutner et al., 1953; Mudd et al., 1953) contain descriptions of the light microscopic cytology and electron microscopy of T2 bacteriophage infection of Escherichia coli, strain B, with particular emphasis on the changes occurring in the chromatinic material, and with the intracellular appearance of possible phage precursors and apparently mature phage progeny. In this paper we show that the cytoplasmic organelles, the mitochondria, in contrast with the nuclei, maintain their integrity throughout the latent period of infection. MATERIALS AND METHODS Escherichia coli, strain B, and phages T2r+ and T2r were used throughout. The phage suspensions, media, basic techniques, and microscopic equipment have been described elsewhere (Beutner et al., 1953; Mudd et al., 1953). In addition, experiments were performed utilizing 2,3,5-triphenyltetrazolium chlorides as an indicator of reduction (Brodie and Gots, 1951, 1952; Kun, 1951; Shelton and Schneider, 1952). The reduction of 2,3,5-triphenyltetrazolium chloride was followed by mixing 3 ml aliquots of deepgrown (unaerated) log phase broth4 cultures of E. coli and 1 ml of a distilled water solution of I This work has been aided by a grant from the United States Atomic Energy Commission, AEC Contract no. AT(30-1)-1342. Aid was also received from the Theresa F. Felsen Memorial Fund.

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