<i>Serratia marcescens</i> SCH909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms

Gambino, Anahí S.; Déraspe, Maxime; Álvarez, Verónica Elizabeth; Quiroga, María Paula; Corbeil, Jacques; Roy, Paul H.; Centrón, Daniela

doi:10.1093/femsle/fnab086

Cited by 3 publications

(2 citation statements)

References 54 publications

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“…Plant-and insectassociated factors, by contrast, tend to be located on GIs. Their role in shaping the genome of S. marcescens was linked to the emergence of MDR (multidrug-resistant) phenotypes (Mataseje et al, 2014;Gambino et al, 2021). Of particular note, Serratia sp.…”

Section: Discussionmentioning

confidence: 99%

The man, the plant, and the insect: shooting host specificity determinants in Serratia marcescens pangenome

Shikov,

Merkushova,

Savina

et al. 2023

Front. Microbiol.

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IntroductionSerratia marcescens is most commonly known as an opportunistic pathogen causing nosocomial infections. It, however, was shown to infect a wide range of hosts apart from vertebrates such as insects or plants as well, being either pathogenic or growth-promoting for the latter. Despite being extensively studied in terms of virulence mechanisms during human infections, there has been little evidence of which factors determine S. marcescens host specificity. On that account, we analyzed S. marcescens pangenome to reveal possible specificity factors.MethodsWe selected 73 high-quality genome assemblies of complete level and reconstructed the respective pangenome and reference phylogeny based on core genes alignment. To find an optimal pipeline, we tested current pangenomic tools and obtained several phylogenetic inferences. The pangenome was rich in its accessory component and was considered open according to the Heaps’ law. We then applied the pangenome-wide associating method (pan-GWAS) and predicted positively associated gene clusters attributed to three host groups, namely, humans, insects, and plants.ResultsAccording to the results, significant factors relating to human infections included transcriptional regulators, lipoproteins, ABC transporters, and membrane proteins. Host preference toward insects, in its turn, was associated with diverse enzymes, such as hydrolases, isochorismatase, and N-acetyltransferase with the latter possibly exerting a neurotoxic effect. Finally, plant infection may be conducted through type VI secretion systems and modulation of plant cell wall synthesis. Interestingly, factors associated with plants also included putative growth-promoting proteins like enzymes performing xenobiotic degradation and releasing ammonium irons. We also identified overrepresented functional annotations within the sets of specificity factors and found that their functional characteristics fell into separate clusters, thus, implying that host adaptation is represented by diverse functional pathways. Finally, we found that mobile genetic elements bore specificity determinants. In particular, prophages were mainly associated with factors related to humans, while genetic islands-with insects and plants, respectively.DiscussionIn summary, functional enrichments coupled with pangenomic inferences allowed us to hypothesize that the respective host preference is carried out through distinct molecular mechanisms of virulence. To the best of our knowledge, the presented research is the first to identify specific genomic features of S. marcescens assemblies isolated from different hosts at the pangenomic level.

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Section: Discussionmentioning

confidence: 99%

The man, the plant, and the insect: shooting host specificity determinants in Serratia marcescens pangenome

Shikov,

Merkushova,

Savina

et al. 2023

Front. Microbiol.

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“…The plasmid paadB from the E. coli TOP10::paadB strain was used for the transformation assays ( Table 1 ). The aadB gene cassette from S. marcescens SCH909 ( Gambino et al., 2021 ) was subcloned from a pCR2.1TOPO vector (Invitrogen, Carlsbad, CA) into the commercial vector pACYC184; paadB is resistant to chloramphenicol due to the background of its vector and to gentamicin due to the Pc promoter contained upstream the aadB gene cassette ( Gambino et al., 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

Carrera Páez,

Olivier,

Gambino

et al. 2024

Front. Cell. Infect. Microbiol.

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There is scarce information concerning the role of sporadic clones in the dissemination of antimicrobial resistance genes (ARGs) within the nosocomial niche. We confirmed that the clinical Escherichia coli M19736 ST615 strain, one of the first isolates of Latin America that harbors a plasmid with an mcr-1 gene, could receive crucial ARG by transformation and conjugation using as donors critical plasmids that harbor blaCTX-M-15, blaKPC-2, blaNDM-5, blaNDM-1, or aadB genes. Escherichia coli M19736 acquired blaCTX-M-15, blaKPC-2, blaNDM-5, blaNDM-1, and aadB genes, being only blaNDM-1 maintained at 100% on the 10th day of subculture. In addition, when the evolved MDR-E. coli M19736 acquired sequentially blaCTX-M-15 and blaNDM-1 genes, the maintenance pattern of the plasmids changed. In addition, when the evolved XDR-E. coli M19736 acquired in an ulterior step the paadB plasmid, a different pattern of the plasmid’s maintenance was found. Interestingly, the evolved E. coli M19736 strains disseminated simultaneously the acquired conjugative plasmids in different combinations though selection was ceftazidime in all cases. Finally, we isolated and characterized the extracellular vesicles (EVs) from the native and evolved XDR-E. coli M19736 strains. Interestingly, EVs from the evolved XDR-E. coli M19736 harbored blaCTX-M-15 though the pDCAG1-CTX-M-15 was previously lost as shown by WGS and experiments, suggesting that EV could be a relevant reservoir of ARG for susceptible bacteria. These results evidenced the genetic plasticity of a sporadic clone of E. coli such as ST615 that could play a relevant transitional link in the clinical dynamics and evolution to multidrug/extensively/pandrug-resistant phenotypes of superbugs within the nosocomial niche by acting simultaneously as a vector and reservoir of multiple ARGs which later could be disseminated.

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Genomic epidemiological investigation of an outbreak of Serratia marcescens neurosurgical site infections associated with contaminated haircutting toolkits in a hospital barber shop

Liu,

Yan,

et al. 2023

Journal of Hospital Infection

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Serratia marcescens SCH909 as reservoir and source of genetic elements related to wide dissemination of antimicrobial resistance mechanisms

Cited by 3 publications

References 54 publications

The man, the plant, and the insect: shooting host specificity determinants in Serratia marcescens pangenome

The man, the plant, and the insect: shooting host specificity determinants in Serratia marcescens pangenome

Sporadic clone Escherichia coli ST615 as a vector and reservoir for dissemination of crucial antimicrobial resistance genes

Genomic epidemiological investigation of an outbreak of Serratia marcescens neurosurgical site infections associated with contaminated haircutting toolkits in a hospital barber shop

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