<i>Smooth</i>, a hnRNP encoding gene, controls axonal navigation in <i>Drosophila</i>

Layalle, Sophie; Coessens, Elise; Ghysen, Alain; Dambly‐Chaudière, Christine

doi:10.1111/j.1365-2443.2005.00822.x

Cited by 16 publications

(14 citation statements)

References 27 publications

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“…Sm is primarily expressed in chemosensory neurons and homozygous sm mutants show defects in axonal arborization of chemosensory neurons and in feeding behavior. These defects may be related to their early death after eclosion (Layalle et al, 2005). Interestingly, hnRNP L and hnRNP A2 are known to bind to the 3’ UTR of glucose transporter-1 mRNA to repress its translation in the glioblastoma cells (Hamilton et al, 1999).…”

Section: Resultsmentioning

confidence: 99%

Misexpression screen delineates novel genes controlling Drosophila lifespan

Paik

Jang²,

Lee³

et al. 2012

Mechanisms of Ageing and Development

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In an initial preliminary screen we identified factors associated with controlling Drosophila aging by examining longevity in adults where EP elements induced over-expression or antisense-RNA at genes adjacent to each insertion. Here, we study 45 EP lines that initially showed at least 10% longer mean lifespan than controls. These 45 lines and a daughterless (da)-Gal4 stock were isogenized into a CS10 wild-type background. Sixteen EP lines corresponding to 15 genes significantly extended lifespan when their target genes were driven by da-Gal4. In each case, the target genes were seen to be over-expressed. Independently derived UAS-gene transgenic stocks were available or made for two candidates: ImpL2 which is ecdysone-inducible gene L2, and CG33138, 1,4-alpha-glucan branching enzyme. With both, adult lifespan was increased upon over-expression via the GeneSwitch inducible Gal4 driver system. Several genes in this set of 15 correspond to previously discovered longevity assurance systems such as insulin/IGF-1 signaling, gene silencing, and autophagy; others suggest new potential mechanisms for the control of aging including mRNA synthesis and maturation, intracellular vesicle trafficking, and neuroendocrine regulation.

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Section: Resultsmentioning

confidence: 99%

Misexpression screen delineates novel genes controlling Drosophila lifespan

Paik

Jang²,

Lee³

et al. 2012

Mechanisms of Ageing and Development

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“…The RNA-binding protein smooth (sm) was also up-regulated in rsh 1 mutants. Mutations in sm have been shown to alter axonal pathfinding Cold Spring Harbor Laboratory Press on May 11, 2018 -Published by learnmem.cshlp.org Downloaded from (Layalle et al 2005). Other genes that were transcriptionally altered in rsh 1 mutants and that would be predicted to influence synaptic connectivity were the Sh potassium channel, the adapter protein Disabled, and the Lapsyn cell adhesion protein.…”

Section: Discussionmentioning

confidence: 99%

Altered gene regulation and synaptic morphology in Drosophila learning and memory mutants

Guan

Buhl²,

Quinn

et al. 2011

Learn. Mem.

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Genetic studies in Drosophila have revealed two separable long-term memory pathways defined as anesthesia-resistant memory (ARM) and long-lasting long-term memory (LLTM). ARM is disrupted in radish (rsh) mutants, whereas LLTM requires CREB-dependent protein synthesis. Although the downstream effectors of ARM and LLTM are distinct, pathways leading to these forms of memory may share the cAMP cascade critical for associative learning. Dunce, which encodes a cAMP-specific phosphodiesterase, and rutabaga, which encodes an adenylyl cyclase, both disrupt short-term memory. Amnesiac encodes a pituitary adenylyl cyclase-activating peptide homolog and is required for middle-term memory. Here, we demonstrate that the Radish protein localizes to the cytoplasm and nucleus and is a PKA phosphorylation target in vitro. To characterize how these plasticity pathways may manifest at the synaptic level, we assayed synaptic connectivity and performed an expression analysis to detect altered transcriptional networks in rutabaga, dunce, amnesiac, and radish mutants. All four mutants disrupt specific aspects of synaptic connectivity at larval neuromuscular junctions (NMJs). Genome-wide DNA microarray analysis revealed ∼375 transcripts that are altered in these mutants, suggesting defects in multiple neuronal signaling pathways. In particular, the transcriptional target Lapsyn, which encodes a leucine-rich repeat cell adhesion protein, localizes to synapses and regulates synaptic growth. This analysis provides insights into the Radish-dependent ARM pathway and novel transcriptional targets that may contribute to memory processing in Drosophila.

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“…In later studies, the sm gene was cloned and several mutant alleles were mapped within the locus 11. More recently, mutations of the sm gene were recovered in genetic screens for factors affecting neuronal development 12,13. The latter report suggests that since null mutations of these pleiotropic genes are homozygous lethal, hypomorphic mutants are better suited to investigate the additional functions mediated by these genes 13…”

Section: Introductionmentioning

confidence: 99%