<i>Sneathia amnii</i>and Maternal Chorioamnionitis and Stillbirth, Mozambique

Vitorino, Pio; Varo, Rosauro; Castillo, Paola; Hurtado, Juan Carlos; Fernandes, Fabíola; Valente, Ana Marta; Mabunda, Rita; Mocumbi, Sibone; Gary, Joy; Jenkinson, Tiffany; Mandomando, Inácio; Blau, Dianna M.; Breiman, Robert F.; Bassat, Quique

doi:10.3201/eid2508.190526

Cited by 11 publications

(9 citation statements)

References 10 publications

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“…Previous in vitro studies showed similar cytokine/chemokine profiles for tested BVAB in the context of human vaginal 87 , 124 – 126 or endometrial epithelial models 108 , 127 . A. vaginae and S. amnii have been also found in patients with inflammatory conditions, such as intrapartum bacteremia 128 , endometritis 129 , urethritis 130 , chorioamnionitis 27 , 131 , and meningitis 132 , further supporting their pathogenic properties. These inflammatory BVAB species may also facilitate acquisition of HIV and other STIs via activation and recruitment of immune cells to the cervical microenvironment 133 .…”

Section: Discussionmentioning

confidence: 86%

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Bacterial vaginosis and health-associated bacteria modulate the immunometabolic landscape in 3D model of human cervix

Łaniewski

Herbst-Kralovetz

2021

npj Biofilms Microbiomes

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Bacterial vaginosis (BV) is an enigmatic polymicrobial condition characterized by a depletion of health-associated Lactobacillus and an overgrowth of anaerobes. Importantly, BV is linked to adverse gynecologic and obstetric outcomes: an increased risk of sexually transmitted infections, preterm birth, and cancer. We hypothesized that members of the cervicovaginal microbiota distinctly contribute to immunometabolic changes in the human cervix, leading to these sequelae. Our 3D epithelial cell model that recapitulates the human cervical epithelium was infected with clinical isolates of cervicovaginal bacteria, alone or as a polymicrobial community. We used Lactobacillus crispatus as a representative health-associated commensal and four common BV-associated species: Gardnerella vaginalis, Prevotella bivia, Atopobium vaginae, and Sneathia amnii. The immunometabolic profiles of these microenvironments were analyzed using multiplex immunoassays and untargeted global metabolomics. A. vaginae and S. amnii exhibited the highest proinflammatory potential through induction of cytokines, iNOS, and oxidative stress-associated compounds. G. vaginalis, P. bivia, and S. amnii distinctly altered physicochemical barrier-related proteins and metabolites (mucins, sialic acid, polyamines), whereas L. crispatus produced an antimicrobial compound, phenyllactic acid. Alterations to the immunometabolic landscape correlate with symptoms and hallmarks of BV and connected BV with adverse women’s health outcomes. Overall, this study demonstrated that 3D cervical epithelial cell colonized with cervicovaginal microbiota faithfully reproduce the immunometabolic microenvironment previously observed in clinical studies and can successfully be used as a robust tool to evaluate host responses to commensal and pathogenic bacteria in the female reproductive tract.

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Section: Discussionmentioning

confidence: 86%

“…Intriguingly, the higher risk for preterm labor have been associated with high levels of particular BV-associated bacteria (BVAB), including Sneathia amnii , in vaginal fluids 20 – 23 . The same BVAB species, S. amnii , has been reported to cause maternal chorioamnionitis, spontaneous abortion, and stillbirth 24 – 27 .…”

Section: Introductionmentioning

confidence: 94%

Bacterial vaginosis and health-associated bacteria modulate the immunometabolic landscape in 3D model of human cervix

Łaniewski

Herbst-Kralovetz

2021

npj Biofilms Microbiomes

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“…PCR and other molecular methods have been shown to be useful for the diagnosis of other fastidious organisms that cause disease in neonates such as Mycoplasma hominis , 42 Ureaplasma parvum , 43 Leptotrichia amnionii , 44 and Sneathia amnii . 45 Epidemiological studies of neonatal serious bacterial infections that only use culture-based methods likely underestimate both the number and variety of infections caused by bacteria.…”

Section: Discussionmentioning

confidence: 99%

Neonatal Paenibacilliosis:Paenibacillus thiaminolyticusas a Novel Cause of Neonatal Sepsis with High Risk of Sequelae in Uganda

Ericson

Burgoine

Kumbakumba

et al. 2022

Preprint

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Paenibacillus thiaminolyticus may be an underdiagnosed cause of neonatal sepsis. We prospectively enrolled 800 neonates presenting with a clinical diagnosis of sepsis at two Ugandan hospitals. Quantitative polymerase chain reaction specific to P. thiaminolyticus and to the Paenibacillus genus were performed on the blood and cerebrospinal fluid (CSF) of 631 neonates who had both specimen types available. Neonates with virus detected in either specimen type were considered to potentially have paenibacilliosis. We described antenatal, perinatal, and neonatal characteristics, presenting signs, and 12-month developmental outcomes for neonates with paenibacillosis vs. clinical sepsis. Median age at presentation was 3 (interquartile range 1, 7) days. Fever (92%), irritability (84%) and seizures (51%) were common. Eleven (30%) had an adverse outcome: 5 (14%) neonates died during the first year of life; 5 of 32 (16%) survivors developed postinfectious hydrocephalus and one (3%) additional survivor had neurodevelopmental impairment without hydrocephalus. These results highlight the need to consider local pathogen prevalence and the possibility of unusual pathogens when determining antibiotic choice for neonatal sepsis.

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“…Several case reports (including ours) have proven the pathogenicity of S. amnii in invasive (mostly gynaecological) infections. S. amnii has been associated with serious obstetric complications, including peripartal bacteraemia [10] and chorioamnionitis, leading to spontaneous abortions and preterm labour [1][2][3][10][11][12][13][14][15][16]. S. amnii has been described as the causative agent of endometritis, salpingitis, tuboovarian abscess and postpartum renal abscess [8,12,17,18].…”

Section: Discussionmentioning

confidence: 99%

“… S. amnii has been associated with serious obstetric complications, including peripartal bacteraemia [10] and chorioamnionitis, leading to spontaneous abortions and preterm labour [1–3, 10–16]. S.…”

Section: Discussionmentioning

confidence: 99%

Sneathia amnii bacteraemia and chorioamnionitis leading to second trimester abortion: a case report

Gruwier

Sprenkels

Hulsbosch

et al. 2021

Access Microbiology

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Background. Sneathia amnii (formerly designated as Leptotrichia amnionii ) was first described in 2002 in the USA. Members of the genus Sneathia can be part of the normal flora of the genitourinary tract, but have been implicated in invasive (mostly gynaecological) infections. Case presentation. To the best of our knowledge, here we present the first case of S. amnii infection in Belgium, in a young woman presenting with fever leading to second trimester septic abortion. Conclusions. Despite its pathogenicity, S. amnii remains an underrated cause of infections due to inherent difficulties with conventional laboratory methods. By extracting the bacterial DNA directly from the blood culture broth and performing a 16S ribosomal RNA gene sequence analysis we succeeded in identifying S. amnii as the most probable cause of the septic abortion in our patient.

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Sneathia amniiand Maternal Chorioamnionitis and Stillbirth, Mozambique

Cited by 11 publications

References 10 publications

Bacterial vaginosis and health-associated bacteria modulate the immunometabolic landscape in 3D model of human cervix

Bacterial vaginosis and health-associated bacteria modulate the immunometabolic landscape in 3D model of human cervix

Neonatal Paenibacilliosis:Paenibacillus thiaminolyticusas a Novel Cause of Neonatal Sepsis with High Risk of Sequelae in Uganda

Sneathia amnii bacteraemia and chorioamnionitis leading to second trimester abortion: a case report

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