SNHG8 promotes cell proliferation, migration, and invasion of nasopharyngeal carcinoma cells as an oncogene through miR-588/HMGA2 axis

Abstract

Nasopharyngeal carcinoma (NC) poses a threat to the life of patients. Long non-coding RNA (LncRNA) is a novel kind of non-coding RNA, which plays a pivotal role through sponge microRNA (miRNA). Abnormal expression of small nucleolar RNA host gene 8 (SNHG8) is involved in various tumors; however, the role of SNHG8 in NC remains unknown. Quantitative real-time PCR (qRT-PCR) and Western blotting was employed to detect the expression levels of SNHG8, miR-588, and high mobility group A2 (HMGA2). Cell proliferation, migration, and invasion were analyzed by CCK-8 and transwell assays. miR-588 binding sites in SNHG8 were predicted by LncBase analysis. Luciferase reporter and RNA pull-down assay were used to confirm the interaction of SNHG8 and miR-588. SNHG8 was highly expressed in NC cells. The prognosis of the patients with NC in the high expression levels of SNHG8 was poorer than that in the low expression levels. The expression of SNHG8 was closely related to tumor size, TNM stage, and distal metastasis. Knockdown of SNHG8 inhibited cell proliferation, migration, and invasion of NC. SNHG8 targeted miR-588. Inhibition of miR-588 could partially reverse the knockdown of SNHG8 in NC cells, and miR-588 targeted HMGA2. In conclusion, SNHG8 promotes proliferation, migration, and invasion of NC cells through miR-588/HMGA2 in NC as an oncogene.

Cuproptosis-Associated lncRNA Establishes New Prognostic Profile and Predicts Immunotherapy Response in Clear Cell Renal Cell Carcinoma

“…All findings suggested that SNHG15 was promising as a biomarker and therapeutic target for cancer patients. Similarly, SNHG8 was considered to be an oncogenic factor and was upregulated in various types of cancer (Yuan et al, 2021), such as gastric cancer, melanoma, nasopharyngeal cancer, and esophageal cancer (Shan et al, 2022b;Luan et al, 2022;Wu et al, 2022;Zhu et al, 2022). LINC00471 was an essential member of the prognostic model of childhood acute myeloid leukemia and esophageal squamous cell carcinoma (Zhang et al, 2019a;Yu et al, 2019).…”

lncRNAs are potential prognostic markers in patients with nasopharyngeal carcinoma in China: A systematic review and meta‑analysis

“…Compared with the results from the combined analysis before and after exclusion, there was no significant change in the association between the high expression of lncRNAs and the T stage, N stage and clinical stage of patients with NPC. The association between the high expression of lncRNAs and the M stage of patients with NPC was affected by the data from the studies by Gao et al 2019( 22 ), Luan et al 2022( 37 ) and Nie et al 2013( 29 ); when these studies were excluded, the I 2 was reduced from 73 to 67%. The data demonstrated that the expression of lncRNAs in the M1 stage was significantly higher than that in the M0 stage (P<0.05) in patients with NPC.…”