Solanum muricatum Ameliorates the Symptoms of Osteogenesis Imperfecta In Vivo

Abstract

Pepino (Solanum muricatum), which is an evergreen plant native to South America, is well‐known for its effects in antioxidation, antidiabetic activity, anti‐inflammation, and antitumor activity. A previous study in our lab indicated that Solanum muricatum (SM) extract promoted osteogenic differentiation by upregulating Wnt and BMP signaling pathway in rat bone marrow stromal cells. The osteogenesis imperfecta (OI) mouse model was used in order to further discover the osteogenic properties of SM extract in the present research. We utilized microCT analysis to collect bone mass and microarchitectural parameters at vertebrae and at femur metaphysis in OI mice. Raman spectrometry was applied to identify change of bone mineral and matrix composition during SM treatment. Finally, collagen synthesis marker PINP and collagen degradation marker CTX were detected using enzyme immunoassay. SM extract could improve the bone mass and microarchitectural parameters both at vertebrae and at femur metaphysis. It also significantly increased the collagen content by promoting its biosynthesis and inhibiting its degradation. By using heterozygous Col1a1Jrt/+ mice as a model of OI, 6 weeks treatment of SM extract could significantly ameliorate the symptoms in OI mice. Thus, SM holds potential for developing new drugs of bone formation and bone remodeling.

Bioscreening and pre-clinical evaluation of the impact of bioactive molecules from Ptychotis verticillata on the multilineage potential of mesenchymal stromal cells towards immune- and inflammation-mediated diseases

“…PPAR-ɣ has been proposed to be a major decision factor in MSC lineage commitment by promoting adipogenesis from MSCs [47]. In parallel, Solanum muricatum extract promoted the osteogenic differentiation of MSCs, ameliorating thus the symptoms of osteogenesis imperfecta in a rat vivo model [48]. The combined administration of Ptychotis verticillata extract and MSCs may represent a new approach to enhance the therapeutic issue.…”

Quercetin promotes osteogenic differentiation and antioxidant responses of mouse bone mesenchymal stem cells through activation of the AMPK/SIRT1 signaling pathway

“…Quercetin is widely used for chronic bronchitis, and also has an adjuvant treatment effect on patients with coronary atherosclerotic heart disease, diabetes, and hypertension (Tamtaji et al, 2019). Our previous research has demonstrated that quercetin, extracted from Solanum muricatum , promoted the osteogenic differentiation of rat BMSCs and thus ameliorated the symptoms of osteogenesis imperfecta in vivo (Wang et al, 2017; Wang et al, 2019). However, the mechanisms underlying the regulatory function of quercetin in BMSCs are poorly understood.…”

Moderate and severe water stress effects on morphological and biochemical traits in a set of pepino (Solanum muricatum) cultivars

“…The pepino fruit is usually eaten as fresh juicy fruit, although some cultivars are used in vegetable salads due to their higher acidity content and herbaceous flavour (Prohens et al, 2002). Different studies found that pepino displays antioxidant, antidiabetic, anti-inflammatory and antitumor properties (Hsu et al, 2011;Shathish and Guruvayoorappan, 2014;Sudha et al, 2011;Virani et al, 2020;Wang et al, 2019;Yue et al, 2019Yue et al, , 2020. One of the most interesting features of pepino is its close phylogenetic relationship with the major crops potato and tomato (Särkinen et al, 2013;Spooner et al, 1993).…”