Spiculopteragia mathevossianiRuchliadev, 1948 is the minor morph ofSpiculopteragia spiculoptera(Gushanskaya, 1931): molecular evidence

Abstract

-Although Spiculopteragia spiculoptera is primarily a parasite of cervids, it can also but less often contaminate domestic livestock. Little is known about its epidemiology and its pathogenicity in domestic ruminants and other unusual cervid species. Its taxonomic status remains unclear since the hypothesis of morphological polymorphism among males has been proposed. However, accurate taxonomy is fundamental in the identification and survey of potentially pathogenic species of parasites. The second internal transcribed spacer of rDNA (ITS-2) and the mitochondrial (mt) DNA-derived ND4 gene were used to study the polymorphism hypothesis for S. spiculoptera. ND4 evolves more quickly than ITS-2 and is considered to be more discriminant in the characterization of closely related species. DNA sequences of ITS-2 and ND4 were studied in 18 individual males of morphological type spiculoptera and in 3 of morphological type of mathevossiani from Red deer (Cervus elaphus), Roe deer (Capreolus capreolus) and Chamois (Rupicapra rupicapra). Intraindividual ITS-2 variations were detected within and between each morphotype of Spiculopteragia but these differences did not separate the two morphs mathevossiani and spiculoptera. Similarly, although ND4 showed a high level of nucleotide substitution, the morphotypes S. mathevossiani and S. spiculoptera were clustered together. Our genetic data support the dimorphic male hypothesis for the species S. spiculoptera. mitochondrial DNA / second internal transcribed spacer (ITS-2) / Spiculopteragia spiculoptera / Spiculopteragia mathevossiani / polymorphism

Türkiye Faunası İçin Karacalarda İki Yeni Nematod Türü: Spiculopteragia spiculoptera (Guschanskaia, 1931) ve Küçük Varyetesi S. (Rinadia) mathevossiani (Ruchliadev, 1948)

“…Nematodes were prepared as temporary whole mounts cleared in phenol -alcohol (80 parts melted phenol crystals and 20 parts absolute ethanol) and examined and measured with a microscope (Eclipse 80i, Nikon Corporation) connected to a digital camera with a liquid crystal display and a measurement specific software (Nikon Digital Sight1 DS-L1). Identification of Spiculopteragia nematodes examined in our material corresponds morphologically to descriptions by the different literatures [2][3][4][5][6][7][8][9] . After examination, specimens were preserved in ethanol-glycerine-formalin protective solution (92 part 70% ethanol, 5 part glycerine, and 3 part commercially available formalin).…”

“…Primarily regarded as a pathogen of wild ruminants, Spiculopteragia species is also reported, but less often, as parasites domestic ruminants. Little is known about its epidemiology and pathogenecity in domestic ruminants and other cervid species 6,[18][19][20] . S. spiculopteragia differ from S. (R.) mathevossiani mainly in a well developed caudal bursa in the male and structure of the spicules and dorsal rib in this study.…”

Molecular genetic conspecificity of Spiculopteragia houdemeri (Schwartz, 1926) and S. andreevae (Dróżdż, 1965) (Nematoda: Ostertagiinae) from wild ruminants in Japan

“…Recent molecular techniques such as allozyme analyses, or genetic analyses of the internal transcribed spacer (ITS) region of the ribosomal RNA gene (rDNA) or of mitochondrial DNA (mtDNA) genes also support the aforementioned hypothesis (Andrews & Beveridge, 1990; Gasnier et al , 1993; Humbert & Cabaret, 1995; Stevenson et al , 1996; Zarlenga et al , 1998; Dallas et al , 2000; Santín-Durán et al , 2002; Liénard et al , 2006). Furthermore, cryptic species have been noted in such polymorphic species in the Ostertagiinae subfamily irrespective of morphotype, e.g.…”

An updated checklist of helminth and protozoan parasites of terrestrial mammals in New Zealand

“…Hence Ostertagia (formerly Skrjabinagia) fyrata, Ostertagia (formerly Skrjabinagia) kolchida, and Cooperia surnabada (formerly C. mcmasteri) continue to be listed as separate species although they are now Z08032; Online publication date 6April Received 29 October 2008accepted 3 February 2009 regarded as polymorphs of Ostertagia ostertagia Ostertagia leptospicularis (formerly O. crimensis) and Cooperia oncophora, respectively (Isenstein 1971;Lancaster & Hong 1990;Lichtenfels & Hoberg 1993). Similarly, while Ostertagia (now Teladorsagia) trifurcata and Ostertagia pinnata (now Teladorsagia davtianf) are also considered to be polymorphs oí Ostertagia (now Teladorsagia) circumcincta, and Apteragia quadrispiculata and Rinadia mathevossiani (=Spiculopteragia mathevossianf) polymorphs of Spiculopteragia asymmetrica (Andrews & Beveridge 1990;Lancaster & Hong 1990) and Spiculopteragia spiculoptera (Lienard et al 2006), they too remain as previously listed. In addition, despite questions regarding its taxonomic validity, and the suggestion that it should be renamed Trichostrongylus lerouxi (Biocca & Chabaud 1984), the use of Trichostrongylus longispicularis in the ox is continued.…”