<i>SPL8</i>, an SBP-Box Gene That Affects Pollen Sac Development in Arabidopsis

Unte, Ulrike S.; Sørensen, Anna; Pesaresi, Paolo; Gandikota, Madhuri; Leister, Dario; Saedler, Heinz; Huijser, Peter

doi:10.1105/tpc.010678

Cited by 237 publications

(225 citation statements)

References 40 publications

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“…In addition, the study of mutants exhibiting stage-specific defects in microsporogenesis and pollen release has further advanced our understanding of the process (Preuss et al, 1993;Sanders et al, 1999;Park and Twell, 2001; Azumi et al, 2002; Steiner-Lange et al, 2003; Unte et al, 2003). Although little is known about how GA controls stamen filament elongation, anther development or microsporogenesis, there have been previous suggestions that GA-signalling components may modulate these processes.…”

Section: Introductionmentioning

confidence: 99%

Gibberellin regulatesArabidopsisfloral development via suppression of DELLA protein function

et al. 2004

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The phytohormone gibberellin (GA) regulates the development and fertility of Arabidopsis flowers. The mature flowers of GA-deficient mutant plants typically exhibit reduced elongation growth of petals and stamens. In addition, GA-deficiency blocks anther development, resulting in male sterility. Previous analyses have shown that GA promotes the elongation of plant organs by opposing the function of the DELLA proteins, a family of nuclear growth repressors. However, it was not clear that the DELLA proteins are involved in the GA-regulation of stamen and anther development. We show that GA regulates cell elongation rather than cell division during Arabidopsis stamen filament elongation. In addition, GA regulates the cellular developmental pathway of anthers leading from microspore to mature pollen grain. Genetic analysis shows that the Arabidopsis DELLA proteins RGA and RGL2 jointly repress petal, stamen and anther development in GA-deficient plants, and that this function is enhanced by RGL1 activity. GA thus promotes Arabidopsis petal, stamen and anther development by opposing the function of the DELLA proteins RGA, RGL1 and RGL2.

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Section: Introductionmentioning

confidence: 99%

Gibberellin regulatesArabidopsisfloral development via suppression of DELLA protein function

et al. 2004

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“…Most likely being a transcription factor (Birkenbihl et al 2005), SPL8 has previously been shown to be required for proper micro-and megasporogenesis as deduced from its semi-sterile mutant phenotype (Unte et al 2003). In this study, we generated SPL8 gain-of-function transgenic lines.…”

Section: Introductionmentioning

confidence: 99%

SPL8, a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis

et al. 2006

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“…To date, only a few functions for SBP domain proteins have been reported, perhaps due to genetic redundancy of closely related family members. In all cases, SBP domain proteins have been implicated in various aspects of plant growth and development, including metal sensing in algae and directing development of leaves, embryos, and floral organs in higher plants (9,(13)(14)(15)(16)(17)(18)(19)(20)(21)(22). Some of these SBP do-main proteins may also be posttranscriptionally regulated by noncoding microRNAs to control their spatial and temporal expression (23)(24)(25).…”

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confidence: 99%

Identification of a Consensus DNA-Binding Site for the Arabidopsis thaliana SBP Domain Transcription Factor, AtSPL14, and Binding Kinetics by Surface Plasmon Resonance

2008

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Proteins with a conserved Cys- and His-rich SQUAMOSA promoter binding protein (SBP) domain are transcription factors restricted to photosynthetic organisms that possess a novel two Zn-finger structure DNA-binding domain. Despite the fact that altered expression of some SBP-encoding genes has profound effects on organism growth and development, little is known about SBP domain protein target genes. Misexpression of the Arabidopsis thaliana AtSPL14 SBP domain gene confers resistance to programmed cell death and modifies plant architecture. A consensus DNA-binding motif for AtSPL14 was identified by systematic evolution of ligands by exponential enrichment (SELEX) or random binding site selection (RBSS). DNA recognized by AtSPL14 contained the core binding motif (GTAC) found for other SBP domain proteins, but mutational analyses indicated that at least one additional flanking nucleotide is necessary for effective AtSPL14−DNA interaction. Comparison of several SBP domain amino acid sequences allows us to hypothesize which specific amino acids might participate in this sequence-specific DNA recognition. Electrophoretic mobility shift assays (EMSA) with mutant AtSPL14 DNA-binding domain proteins indicated that not all of the Zn2+ ion coordinating ligands in the second Zn structure are strictly required for DNA binding. Surface plasmon resonance (SPR) was used to evaluate AtSPL14 in vitro binding kinetics for comparison of equilibrium binding constants with other SBP domain proteins. These data provide a strong basis for further experiments aimed at defining and distinguishing the sets of genes regulated by the closely related SBP domain family members.

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SPL8, an SBP-Box Gene That Affects Pollen Sac Development in Arabidopsis

Cited by 237 publications

References 40 publications

Gibberellin regulatesArabidopsisfloral development via suppression of DELLA protein function

Gibberellin regulatesArabidopsisfloral development via suppression of DELLA protein function

SPL8, a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis

Identification of a Consensus DNA-Binding Site for the Arabidopsis thaliana SBP Domain Transcription Factor, AtSPL14, and Binding Kinetics by Surface Plasmon Resonance

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