<i>Staphylococcus lugdunensis</i>: an unusual and aggressive cause of infective endocarditis

Umanzor, Eduardo Josué Flores; Antonio, Rodolfo San; Brítez, Gustavo Jiménez; Caldentey, Guillem

doi:10.1136/bcr-2016-217156

Cited by 4 publications

(2 citation statements)

References 2 publications

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“…In addition, as all these three CoNS species belong to the S. epidermidis group as part of the normal skin flora with similar pathogenicity [20] it is not mandatory to identify these CoNS on the species level. Worth mentioning is the inclusion of S. lugdunensis in the Genmark ePlex® panel as it is a common pathogen with species specific virulence factors [21] associated with aggressive causes of infective endocarditis [22][23][24]. In addition, two E. faecalis isolates were misidentified as E. faecium.…”

Section: Identification Of Gram-positive Bsi Pathogensmentioning

confidence: 99%

Evaluation of two rapid molecular test systems to establish an algorithm for fast identification of bacterial pathogens from positive blood cultures

Oberhettinger

Zieger

Autenrieth

et al. 2020

Eur J Clin Microbiol Infect Dis

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Fast identification of pathogens directly from positive blood cultures is of highest importance to supply an adequate therapy of bloodstream infections (BSI). There are several platforms providing molecular-based identification, detection of antimicrobial resistance genes, or even a full antimicrobial susceptibility testing (AST). Two of such test systems allowing rapid diagnostics were assessed in this study: The Biofire FilmArray® and the Genmark ePlex®, both fully automated test system with a minimum of hands-on time. Overall 137 BSI episodes were included in our study and compared to conventional culture–based reference methods. The FilmArray® is using one catridge including a panel for the most common bacterial and fungal BSI pathogens as well as selected resistance markers. The ePlex® offers three different cartridges for detection of Gram-positives, Gram-negatives, and fungi resulting in a broader panel including also rare pathogens, putative contaminants, and more genetic resistance markers. The FilmArray® and ePlex® were evaluated for all 137 BSI episodes with FilmArray® detecting 119 and ePlex® detecting 128 of these. For targets on the respective panel of the system, the FilmArray® generated a sensitivity of 98.9% with 100% specificity on Gram-positive isolates. The ePlex® system generated a sensitivity of 94.7% and a specificity of 90.7% on Gram-positive isolates. In each case, the two systems performed with 100% sensitivity and specificity for the detection of Gram-negative specimens covered by each panel. In summary, both evaluated test systems showed a satisfying overall performance for fast pathogen identification and are beneficial tools for accelerating blood culture diagnostics of sepsis patients.

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Section: Identification Of Gram-positive Bsi Pathogensmentioning

confidence: 99%

Evaluation of two rapid molecular test systems to establish an algorithm for fast identification of bacterial pathogens from positive blood cultures

Oberhettinger

Zieger

Autenrieth

et al. 2020

Eur J Clin Microbiol Infect Dis

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“…S. lugdunensis is a life-threatening pathogen and causes prosthetic device-associated endocarditis, which has a high mortality rate ( Flores Umanzor et al., 2016 ; Ishiekwene et al., 2017 ). Previous studies have shown that S. lugdunensis can interact with platelets through binding of the surface protein vWbl to vWf ( Nilsson et al., 2004 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene

Lin

Cheng

Chang

2021

Front. Cell. Infect. Microbiol.

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The von Willebrand factor binding protein in Staphylococcus lugdunensis (vWbl) comprises four major regions: the signal peptide (S), the non-repetitive (A) region, the repeat (R) region, and the wall-associated (W) region. Previous studies have demonstrated that the R region contains 10 copies of repeating sequences; however, we reveal that the copy number of repeats in the vWbl gene varies among different S. lugdunensis isolates. In this study, an epidemiological surveillance was conducted to determine whether the copy number of repeats in vWbl in different isolates of S. lugdunensis correlates with their infectivity. The number of repeats was estimated in a total of 212 isolates, consisting of 162 isolates of oxacillin-sensitive S. lugdunensis (OSSL) and 50 isolates of oxacillin-resistant S. lugdunensis (ORSL). Our data showed that 72.5% (116/162) of OSSL isolates contained 9 (25, 15.4%), 12 (43, 26.5%), or 13 (48, 29.6%) repeats, and 90% (45/50) of ORSL isolates had 9 (32, 64%) or 13 (13, 26%) repeats. In addition, 89.6% (26 of 29) of the sequence type (ST)27 strain had 12 repeats, and 86.8% (13 of 15) of the ST4 strain had 14 repeats. Twenty-seven of the 28 isolates with nine repeats were of the staphylococcal cassette chromosome mec (SCCmec) V or Vt type and belonged to ST3, and all isolates with 13 repeats were of SCCmec II type and belonged to ST6. All isolates with nine repeats had a stop codon at the 18th codon of the third repeat, suggesting that these isolates coded for nonfunctional vWbl. Further, western blot analysis confirmed that all strains translated vWbl, and only vWbl proteins coded by genes with nine repeats were exported outside the cell. These results suggest that number of vWbl repeats in S. lugdunensis have clonal specificities and may correlate with potential pathogenicity.

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Correction to: Evaluation of two rapid molecular test systems to establish an algorithm for fast identification of bacterial pathogens from positive blood cultures

Oberhettinger

Zieger

Autenrieth

et al. 2020

Eur J Clin Microbiol Infect Dis

View full text Add to dashboard Cite

Staphylococcus lugdunensis: an unusual and aggressive cause of infective endocarditis

Cited by 4 publications

References 2 publications

Evaluation of two rapid molecular test systems to establish an algorithm for fast identification of bacterial pathogens from positive blood cultures

Evaluation of two rapid molecular test systems to establish an algorithm for fast identification of bacterial pathogens from positive blood cultures

Molecular Epidemiological Survey of Staphylococcus lugdunensis Isolates With Variable Number of Repeats in the von Willebrand Factor-Binding Protein Gene

Correction to: Evaluation of two rapid molecular test systems to establish an algorithm for fast identification of bacterial pathogens from positive blood cultures

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