<i>Stenotrophomonas maltophilia</i> biofilm: its role in infectious diseases

Flores-Treviño, Samantha; Bocanegra‐Ibarias, Paola; Camacho-Ortíz, Adrián; Morfín‐Otero, Rayo; Salazar-Sesatty, Humberto Antonio; Garza‐González, Elvira

doi:10.1080/14787210.2019.1685875

Cited by 89 publications

(92 citation statements)

References 111 publications

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“…Interestingly, the same concentration of PYED-1 has a synergistic effect with gentamicin or amikacin on bacterial growth inhibition. This is in agreement with other studies showing that S. maltophilia biofilm formation plays a notable role in the development of antibiotic resistance [20][21][22].…”

Section: Effects Of Pyed-1 On the Formation Of S Maltophilia Biofilmsupporting

confidence: 93%

“…Most S. maltophilia strains form biofilms on several biotic and abiotic surfaces, and this greatly contributes to the pathogenicity of these bacteria [20][21][22]. Thus, the antibiofilm properties of PYED-1 against S. maltophilia K279a were investigated.…”

Section: Effects Of Pyed-1 On the Formation Of S Maltophilia Biofilmmentioning

confidence: 99%

“…However, the allergenic reaction, the intolerance, and the increasing rates of resistance limit the use of Trimethoprim-sulfamethoxazole [18,19]. S. maltophilia infections are also difficult to treat due to the ability of this bacterium to form highly structured and multilayered biofilms [20][21][22]. Because of the potential for resistance development, there is need of novel antimicrobials for S. maltophilia infection treatment.…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial and Antivirulence Activity of Glucocorticoid PYED-1 against Stenotrophomonas maltophilia

et al. 2020

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Stenotrophomonas maltophilia, an environmental Gram-negative bacterium, is an emerging nosocomial opportunistic pathogen that causes life-threatening infections in immunocompromised patients and chronic pulmonary infections in cystic fibrosis patients. Due to increasing resistance to multiple classes of antibiotics, S. maltophilia infections are difficult to treat successfully. This makes the search for new antimicrobial strategies mandatory. In this study, the antibacterial activity of the heterocyclic corticosteroid deflazacort and several of its synthetic precursors was tested against S. maltophilia. All compounds were not active against standard strain S. maltophilia K279a. The compound PYED-1 (pregnadiene-11-hydroxy-16α,17α-epoxy-3,20-dione-1) showed a weak effect against some S. maltophilia clinical isolates, but exhibited a synergistic effect with aminoglycosides. PYED-1 at sub-inhibitory concentrations decreased S. maltophilia biofilm formation. Quantitative real-time polymerase chain reaction (RT-qPCR) analysis demonstrated that the expression of biofilm- and virulence- associated genes (StmPr1, StmPr3, sphB, smeZ, bfmA, fsnR) was significantly suppressed after PYED-1 treatment. Interestingly, PYED-1 also repressed the expression of the genes aph (3′)-IIc, aac (6′)-Iz, and smeZ, involved in the resistance to aminoglycosides.

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Section: Effects Of Pyed-1 On the Formation Of S Maltophilia Biofilmsupporting

confidence: 93%

Section: Effects Of Pyed-1 On the Formation Of S Maltophilia Biofilmmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibacterial and Antivirulence Activity of Glucocorticoid PYED-1 against Stenotrophomonas maltophilia

et al. 2020

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“…Approximatively, 10% of the French CF patients carry in their airways S. maltophilia , and 30 % carry Aspergillus (French, 2019 ), with common co-infections, as recently shown in a very large cohort of CF patients (Granchelli et al, 2018 ). Treatment of these pathogens in chronic respiratory diseases is often difficult due to their multidrug-resistant nature, especially for S. maltophilia , and to their biofilm-forming ability (Flores-Treviño et al, 2019 ). The presence of microbial aggregates and biofilms have already been observed in the respiratory tract of CF patients, which is a favorable environment for biofilm formation (Bjarnsholt et al, 2009 ; Ramage et al, 2011 ; Kragh et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

Modulated Response of Aspergillus fumigatus and Stenotrophomonas maltophilia to Antimicrobial Agents in Polymicrobial Biofilm

Roisin

Melloul

Woerther

et al. 2020

Front. Cell. Infect. Microbiol.

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Introduction: The complexity of biofilms constitutes a therapeutic challenge and the antimicrobial susceptibility of fungal-bacterial biofilms remains poorly studied. The filamentous fungus Aspergillus fumigatus (Af) and the Gram-negative bacillus Stenotrophomonas maltophilia (Sm) can form biofilms and can be co-isolated from the airways of cystic fibrosis (CF) patients. We previously developed an in vitro biofilm model which highlighted the antibiosis effect of Sm on Af, which was dependent on the bacterial fitness. The aim of the present study was to investigate the in vitro susceptibility of Af and Sm in mono- or polymicrobial biofilms to five antimicrobial agents alone and in two-drug combinations. Methods: Af and Sm clinical reference strains and two strains from CF sputa were tested through a planktonic and biofilm approaches. Af, Sm, or Af-Sm susceptibilities to amphotericin B (AMB), itraconazole (ITC), voriconazole (VRC), levofloxacin (LVX), and rifampicin (RFN) were evaluated by conventional planktonic techniques, crystal violet, XTT, qPCR, and viable plate count. Results: Af planktonic cells and biofilms in formation were more susceptible to AMB, ITC, and VRC than Af mature biofilms. Af mature biofilms were susceptible to AMB, but not to ITC and VRC. Based on viable plate count, a lower concentration of LVX and RFN was required to reduce Sm cell numbers on biofilms in formation compared with mature biofilms. The antibiosis effect of Sm on Af growth was more pronounced for the association of CF strains that exhibited a higher fitness than the reference strains. In Af-Sm biofilms, the fungal susceptibility to AMB was increased compared with Af biofilms. In contrast, the bacterial susceptibility to LVX decreased in Af-Sm biofilms and was fungal biomass-dependent. The combination of AMB (64 μg/mL) with LVX or RFN (4 μg/mL) was efficient to impair Af and Sm growth in the polymicrobial biofilm. Conclusion: Sm increased the Af susceptibility to AMB, whereas Af protected Sm from LVX. Interactions between Af and Sm within biofilms modulate susceptibility to antimicrobial agents, opening the way to new antimicrobial strategies in CF patients.

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“…S. maltophilia produces high levels of biofilm in most infections, which helps the bacteria to facilitate colonization, evade the host immune response and resist the effect of the antibiotics [5]. Infections with biofilm-producing strains are difficult to treat and eradicate, as they rarely respond to conventional antibiotics.…”

Section: Introductionmentioning

confidence: 99%

Discrimination of biofilm-producing Stenotrophomonas maltophilia clinical strains by matrix-assisted laser desorption ionization–time of flight

et al. 2020

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Stenotrophomonas maltophilia is a Gram-negative drug-resistant pathogen responsible for healthcare-associated infections. The aim was to search for biomarker peaks that could rapidly detect biofilm production in S. maltophilia clinical isolates obtained from two tertiary care hospitals in Mexico. Isolates were screened for the presence of biofilm-associated genes, in which the fsnR gene was associated with biofilm production (p = 0.047), whereas the rmlA+ genotype was associated with the rpfF- genotype (p = 0.017). Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) mass spectra comparison yielded three potential biomarker peaks (4661, 6074, and 6102 m/z) of biofilm-producing rmlA+ and rpfF- genotypes with >90% sensitivity (p<0.001). MALDI-TOF MS analyses showed a correlation between the relative abundance of 50S ribosomal proteins (L30 and L33) and the presence of the fnsR, rmlA and rpfF-2 genes, suggested to play a role in biofilm formation. Isolates obtained in the intensive care unit showed low clonality, suggesting no transmission within the hospital ward. The detection of biomarkers peaks by MALDI-TOF MS could potentially be used to early recognize and discriminate biofilm-producing S. maltophilia strains and aid in establishing appropriate antibiotic therapy.

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Stenotrophomonas maltophilia biofilm: its role in infectious diseases

Cited by 89 publications

References 111 publications

Antibacterial and Antivirulence Activity of Glucocorticoid PYED-1 against Stenotrophomonas maltophilia

Antibacterial and Antivirulence Activity of Glucocorticoid PYED-1 against Stenotrophomonas maltophilia

Modulated Response of Aspergillus fumigatus and Stenotrophomonas maltophilia to Antimicrobial Agents in Polymicrobial Biofilm

Discrimination of biofilm-producing Stenotrophomonas maltophilia clinical strains by matrix-assisted laser desorption ionization–time of flight

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