<i>Streptoccocus faecalis</i> endocarditis: an emerging problem?

Welsby, Philip D

doi:10.1136/pgmj.54.631.321

Cited by 2 publications

(1 citation statement)

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“…There is evidence that its incidence is increasing especially in the elderly (Welsby, 1978). Enterococci are generally resistant to many antibiotics (Moellering et ul., 1979;Mederski-Samoraj and Murray, 1983;Wilson and Geraci, 1983) and the treatment of enterococcal endocarditis requires extended periods of antibiotic therapy (Welsby, 1978;Wilson and Geraci, 1983).…”

Section: Introductionmentioning

confidence: 99%

Antigenic composition of an endocarditis-associated isolate of Streptococcus faecalis and identification of its glycoprotein antigens by ligand blotting with lectins

Aitchison¹,

Lambert²,

Farrell³

1986

Journal of Medical Microbiology

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Summary. The antigenic composition of an endocarditis-associated isolate of Streptococcus faecalis was studied by immunoblotting of whole cells and cell walls from sodium-dodecyl sulphate polyacrylamide gels on to nitrocellulose and detection with serum from patients and hyperimmune rabbit serum. A major envelope protein antigen of mol. wt 53 x lo3 detected with patient's serum was also present in three urinary strains of Str. faecalis and a laboratory strain of Str. faecalis ss. zymogenes but not in Staphylococcus aureus. Other common antigens of Str. faecalis were of mol. wt ( lo3) 65,63, 56,49.5,30 and 21. Two other protein antigens (43 and 37 x 10' mol. wt) reacted strongly with asparagus pea lectin-peroxidase conjugate indicating the presence of fucosyl residues. Other lectin-peroxidase conjugates were used to demonstrate the presence of various glycosyl residues on envelope proteins. Growth of Str. faecalis in serum to mimic in-vivo growth conditions in endocarditis infections dramatically altered the antigenic patterns. Only two major antigens of mol. wt ( lo3) 56 and 53 reacted with sera from endocarditis patients. These antigens may, therefore, be of diagnostic or protective potential.

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