<i>Streptococcus agalactiae</i>-induced autophagy of bovine mammary epithelial cell <i>via</i> PI3K/AKT/mTOR pathway

Qi, Mengzhu; Geng, Hao; Geng, Nan; Cui, Yukun; Qi, Changxi; Cheng, Guodong; Song, Kaimin; Hu, Liping; Liu, Yongxia; Liu, Jianzhu; Han, Bo

doi:10.1017/s0022029922000243

Cited by 9 publications

(5 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…S. dysgalactiae can not only form biofilm but also invade and survive within mammary epithelial cells, and persist for long periods, which can avoid the bactericidal action of antibiotics, resulting in persistent infection ( Calvinho and Oliver, 1998 ). Streptococcus also can induce autophagy to increase inflammatory reactions of bovine mammary epithelial cells ( Chen et al, 2022 ; Qi et al, 2022 ). Because of the low intracellular accumulation and special intracellular environmental effects, it is difficult for the antibiotics to play a good bactericidal effect in intracellular.…”

Section: Discussionmentioning

confidence: 99%

Effect of NZ2114 against Streptococcus dysgalactiae biofilms and its application in murine mastitis model

Yang

Zhang²,

Mao³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

Bovine mastitis caused by Streptococcus dysgalactiae (S. dysgalactiae) is usually treated with antibiotics, which may potentially increase drug resistance as the abuse. NZ2114, a variant of fungal defensin plectasin, displayed a potent antibacterial activity against S. dysgalactiae. The inhibition/eradication effect of the antimicrobial peptide NZ2114 on the early/mature biofilm of S. dysgalactiae CVCC 3938 was evaluated, as well as the elimination of bacteria in mature biofilms. In this study, NZ2114 displayed potent antibacterial activity against S. dysgalactiae CVCC 3938 and three clinical isolated S. dysgalactiae strains (0.11-0.45 μM). The early biofilm inhibition of S. dysgalactiae CVCC 3938 was 55.5–85.9% after treatment with NZ2114 at concentrations of 1–16 × MIC, which was better than that of vancomycin at the same concentration. The mature biofilm eradication rate was up to 92.7–97.6% with the increasing concentration (2–16 × MIC) of NZ2114, and the eradication rate did not change significantly with further increase of NZ2114 concentration, while the biofilm eradication rate of vancomycin-treated group at the same concentration remained at 92.5%. NZ2114 reduced the number of persister bacteria in biofilm. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) further demonstrated that NZ2114 could effectively reduce the biofilm thickness and bacterial number of S. dysgalactiae CVCC 3938. In vivo therapeutic effect of NZ2114 on murine mastitis model showed that NZ2114 was better than vancomycin in alleviating mammary gland inflammation by regulating cytokines production, inhibiting bacterial proliferation, and reducing the number of mammary gland bacteria. These data suggested that NZ2114 is a potential peptide candidate for the treatment of mastitis.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of NZ2114 against Streptococcus dysgalactiae biofilms and its application in murine mastitis model

Yang

Zhang²,

Mao³

et al. 2022

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…Our preliminary studies did not confirm that miR-383 is upregulated in mastitic milk; therefore, this molecule was excluded from the experiment. However, other studies found that miR-383 was significantly upregulated in CMT-positive milk ( 18 ) and in mammary epithelial cells in Streptococcus agalactiae -induced mastitis ( 26 ).…”

Section: Discussionmentioning

confidence: 98%

Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland

Smulski,

Pszczoła,

Stachowiak

et al. 2023

Journal of Veterinary Research

View full text Add to dashboard Cite

Introduction MicroRNAs (miRNAs), a class of noncoding small RNAs, have been recognised as potential biomarkers of mammary gland conditions, including bovine mastitis diagnosis. The aim of this study was to quantify selected miRNAs in the milk of mastitic cows. Material and Methods Milk samples (n = 90) were collected from healthy and mastitic dairy cows originating from local dairy cattle farms located in the west of Poland. MicroRNAs of the miR-21a, miR-92a, miR-146a and miR-383 species were quantified using the highly sensitive droplet digital PCR method. Direct measurement of somatic cell count (SCC) was performed using a cell counter. Cows were divided into three groups: those with an SCC below 200,000/mL were designated Low (n = 25), those with an SCC between 200,000 and 999,999 were Medium (n = 34), and those with an SCC of 1,000,000 or higher were High (n = 31). Microbiological analyses were performed using standard culture testing. Results The level of miR-383 was very low and this miRNA was excluded from analysis. The miR-92a was used to normalise miR-21a and miR-146a expression levels. The obtained results of expression of miR-21a and miR-146a correlated with somatic cell number (R = 0.53 and 0.79, respectively). Conclusion These results show that ddPCR is a useful method for quantifying miRNAs in raw cow milk. It seems that miR-146a is a promising marker for bovine mastitis, although further studies are needed to select a panel of miRNAs that can be used in mastitis monitoring in Poland.

show abstract

“…Moreover, autophagy also plays a role in the development of mastitis. Evidence suggests that pathogens, such as Streptococcus agalactiae, induce autophagy through the PI3K/ATK/mTOR pathway in bovine mammary epithelial cells (BMECs) [37], and Streptococcus lutetiensis induces autophagy through oxidative stress in BMECs [38]. Additionally, taurine inhibits the inflammatory response induced by Streptococcus uberis by regulating autophagy in MAC-T cells [39].…”

Section: Discussionmentioning

confidence: 99%

Enterogenic Stenotrophomonas maltophilia migrates to the mammary gland to induce mastitis by activating the calcium-ROS-AMPK-mTOR-autophagy pathway

He,

Zhao,

et al. 2023

J Animal Sci Biotechnol

View full text Add to dashboard Cite

Background Mastitis is an inflammatory disease of the mammary gland that has serious economic impacts on the dairy industry and endangers food safety. Our previous study found that the body has a gut/rumen-mammary gland axis and that disturbance of the gut/rumen microbiota could result in ‘gastroenterogenic mastitis’. However, the mechanism has not been fully clarified. Recently, we found that long-term feeding of a high-concentrate diet induced mastitis in dairy cows, and the abundance of Stenotrophomonas maltophilia (S. maltophilia) was significantly increased in both the rumen and milk microbiota. Accordingly, we hypothesized that ‘gastroenterogenic mastitis’ can be induced by the migration of endogenous gut bacteria to the mammary gland. Therefore, this study investigated the mechanism by which enterogenic S. maltophilia induces mastitis. Results First, S. maltophilia was labelled with superfolder GFP and administered to mice via gavage. The results showed that treatment with S. maltophilia promoted the occurrence of mastitis and increased the permeability of the blood-milk barrier, leading to intestinal inflammation and intestinal leakage. Furthermore, tracking of ingested S. maltophilia revealed that S. maltophilia could migrate from the gut to the mammary gland and induce mastitis. Subsequently, mammary gland transcriptome analysis showed that the calcium and AMPK signalling pathways were significantly upregulated in mice treated with S. maltophilia. Then, using mouse mammary epithelial cells (MMECs), we verified that S. maltophilia induces mastitis through activation of the calcium-ROS-AMPK-mTOR-autophagy pathway. Conclusions In conclusion, the results showed that enterogenic S. maltophilia could migrate from the gut to the mammary gland via the gut-mammary axis and activate the calcium-ROS-AMPK-mTOR-autophagy pathway to induce mastitis. Targeting the gut-mammary gland axis may also be an effective method to treat mastitis.

show abstract

Streptococcus agalactiae-induced autophagy of bovine mammary epithelial cell via PI3K/AKT/mTOR pathway

Cited by 9 publications

References 33 publications

Effect of NZ2114 against Streptococcus dysgalactiae biofilms and its application in murine mastitis model

Effect of NZ2114 against Streptococcus dysgalactiae biofilms and its application in murine mastitis model

Droplet digital PCR quantification of selected microRNAs in raw mastitic cow’s milk from the west of Poland

Enterogenic Stenotrophomonas maltophilia migrates to the mammary gland to induce mastitis by activating the calcium-ROS-AMPK-mTOR-autophagy pathway

Contact Info

Product

Resources

About