<i>Streptococcus pyogenes</i>
            Infection in Mouse Skin Leads to a Time-Dependent Up-Regulation of Protein H Expression

Smith, Tara C.; Sledjeski, Darren D.; Boyle, Michael D. P.

doi:10.1128/iai.71.10.6079-6082.2003

Cited by 9 publications

(6 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The second hit represents SNPs in the intergenic region upstream of both IgG-binding protein H (sph) and nrdI (ribonucleotide reductase). In support of these findings, previous work in murine models have found differential expression of sph during invasive disease [25][26][27] , but little to no expression outside of this niche 28 . If these k-mers were found to affect expression of the IgG-binding protein, this would be a plausible genetic mechanism affecting pathogenesis and invasive propensity 29 .…”

Section: Resultssupporting

confidence: 74%

Sequence element enrichment analysis to determine the genetic basis of bacterial phenotypes

Lees¹

2016

Preprint

View full text Add to dashboard Cite

Bacterial genomes vary extensively in terms of both gene content and gene sequence. This plasticity hampers the use of traditional SNP-based methods for identifying all genetic associations with phenotypic variation. Here we introduce a computationally scalable and widely applicable statistical method (SEER) for the identification of sequence elements that are significantly enriched in a phenotype of interest. SEER is applicable to tens of thousands of genomes by counting variable-length k-mers using a distributed string-mining algorithm. Robust options are provided for association analysis that also correct for the clonal population structure of bacteria. Using large collections of genomes of the major human pathogens Streptococcus pneumoniae and Streptococcus pyogenes, SEER identifies relevant previously characterized resistance determinants for several antibiotics and discovers potential novel factors related to the invasiveness of S. pyogenes. We thus demonstrate that our method can answer important biologically and medically relevant questions.

show abstract

Section: Resultssupporting

confidence: 74%

Sequence element enrichment analysis to determine the genetic basis of bacterial phenotypes

Lees¹

2016

Preprint

View full text Add to dashboard Cite

show abstract

“…In 2003, it was reported that a gene encoding protein H is found in approximately one‐third of the tested clinical M1 serotype isolates (Smith et al ., 2003a). Importantly the protein H expression was induced upon passage in mice (Smith et al ., 2003a, b). Based on these findings, it is tempting to speculate that such the interaction between protein H and β 2 GPI is involved in the generation of APS.…”

Section: Discussionmentioning

confidence: 99%

The antibacterial activity of peptides derived from human beta‐2 glycoprotein I is inhibited by protein H and M1 protein from Streptococcus pyogenes

Nilsson

Wasylik

Mörgelin

et al. 2007

Molecular Microbiology

View full text Add to dashboard Cite

SummaryDuring the last years, the importance of antibacterial peptides has attracted considerable attention. We report here that peptides derived from the fifth domain of beta-2 glycoprotein I (b2GPI), a human heparin binding plasma protein, have antibacterial activities against Gram-positive and Gram-negative bacteria. Streptococcus pyogenes, an important human pathogen that can survive and grow in human blood, has developed mechanisms to escape the attack by these peptides. Thus, protein H and M1 protein, two surface proteins of the highly pathogenic S. pyogenes AP1 strain, bind full-length b2GPI and thereby prevent the processing of b2GPI by proteases from polymorphonuclear neutrophils (PMNs) into antibacterial peptides. In addition, protein H and M1 protein, released from the bacterial cell wall by PMN-derived proteases, bind to, and inhibit the activity of, b2GPI-derived antibacterial peptides. Taken together, the data suggest that the interaction between the streptococcal proteins and b2GPI or b2GPI-derived peptides presents a novel mechanism to resist an antibacterial attack by b2GPI-cleavage products.

show abstract

“…Coinjection of bacteria with a foreign substance (e.g., Cytodex ™ beads) is often employed to further enhance the inflammatory response. In the mouse skin air sac model, GAS are injected subcutaneously; this model can be used to study bacterial dissemination from a focal infection site to distal organs [89]. In another mouse skin infection model, GAS are applied topically following the complete removal of the epidermis by tape stripping; unusually high nonphysiologic inoculum doses (10 7 CFU) of GAS are employed here as well [90].…”

Section: Animal Models For Superficial Gas Infection Of the Skinmentioning

confidence: 99%

Tissue Tropisms in Group a Streptococcal Infections

Bessen

Lizano

2010

Future Microbiol.

View full text Add to dashboard Cite

Group A Streptococcus (GAS) is a human-specific pathogen that is highly prevalent throughout the world. The vast majority of GAS infections lead to a mild disease involving the epithelial surfaces of either the throat or skin. The concept of distinct sets of ‘throat’ and ‘skin’ strains of GAS has long been conceived. From an ecological standpoint, the epithelium of the throat and skin are important because it is where the organism is most successful in reproducing and transmitting to new hosts. This article examines key features of the epidemiology, population biology and molecular pathogenesis that underlie the tissue site preferences for infection exhibited by GAS, with an emphasis on work from our laboratory on skin tropisms. Recombinational replacement with orthologous gene forms, following interspecies transfer, appears to be an important genetic step leading up to the exploitation of new niches by GAS.

show abstract

Streptococcus pyogenes Infection in Mouse Skin Leads to a Time-Dependent Up-Regulation of Protein H Expression

Cited by 9 publications

References 20 publications

Sequence element enrichment analysis to determine the genetic basis of bacterial phenotypes

Sequence element enrichment analysis to determine the genetic basis of bacterial phenotypes

The antibacterial activity of peptides derived from human beta‐2 glycoprotein I is inhibited by protein H and M1 protein from Streptococcus pyogenes

Tissue Tropisms in Group a Streptococcal Infections

Contact Info

Product

Resources

About