<i>Streptomyces</i> sp. MUM273b: A mangrove‐derived potential source for antioxidant and UVB radiation protectants

Tan, Loh Teng‐Hern; Mahendra, Camille Keisha; Yow, Yoon‐Yen; Chan, Kok‐Gan; Khan, Tahir Mehmood; Lee, Learn‐Han; Goh, Bey Hing

doi:10.1002/mbo3.859

Cited by 35 publications

(37 citation statements)

References 108 publications

(137 reference statements)

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“…It is not surprising to find Streptomyces from stressful ecosystem such as mangrove forest, produces secondary metabolites to assist in the defence mechanisms, particularly antioxidants are used to prevent oxidative damage. The antioxidant potential of several mangrove Streptomyces in Malaysia have been reported [27][28][29]. To date, the mangrove forest remains largely an understudied ecosystem with respect to investigating their secondary metabolites which are valuable sources for future development of therapeutics [24].…”

Section: Introductionmentioning

confidence: 99%

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

et al. 2020

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There is an urgent need to search for new antibiotics to counter the growing number of antibiotic-resistant bacterial strains, one of which is methicillin-resistant Staphylococcus aureus (MRSA). Herein, we report a Streptomyces sp. strain MUSC 125 from mangrove soil in Malaysia which was identified using 16S rRNA phylogenetic and phenotypic analysis. The methanolic extract of strain MUSC 125 showed anti-MRSA, anti-biofilm and antioxidant activities. Strain MUSC 125 was further screened for the presence of secondary metabolite biosynthetic genes. Our results indicated that both polyketide synthase (pks) gene clusters, pksI and pksII, were detected in strain MUSC 125 by PCR amplification. In addition, gas chromatography-mass spectroscopy (GC-MS) detected the presence of different chemicals in the methanolic extract. Based on the GC-MS analysis, eight known compounds were detected suggesting their contribution towards the anti-MRSA and anti-biofilm activities observed. Overall, the study bolsters the potential of strain MUSC 125 as a promising source of anti-MRSA and antibiofilm compounds and warrants further investigation.

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Section: Introductionmentioning

confidence: 99%

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

et al. 2020

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“…Studies demonstrated that radical scavengers like vitamin E, in the form of tocopherol and tocotrienol, can protect the glutamate-injured cells from oxidative stress. Other than vitamin E, other sources such as microbial resources were also demonstrating strong antioxidants [116][117][118][119][120][121][122][123][124][125][126] and neuroprotective properties [127][128][129][130][131][132] such as metal chelating and radical scavenging potentials [133][134][135][136][137][138][139][140] . In conclusion, neuronal cell seemed to be the most vulnerable brain cells toward glutamate toxicity.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Insight of Neurodegenerative Diseases and The Role of Neurotoxin Agents — Glutamate

Yap¹,

Lye²,

Tan³

2020

Prog Micobes Mol Biol

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The increased concentration of extracellular glutamate has been reported to play a key role in most of the neurodegenerative diseases, such as Parkinson’s disease and Alzheimer’s disease, even though its importance as an amino acid neurotransmitter in mammalian. Glutamate toxicity, which can be caused by excessive intake of monosodium glutamate (MSG), is the major contributor to pathological neuronal cell death. It causes neuronal dysfunction and degeneration in the central nervous system (CNS). Glutamate neurotoxicity can be categorized into two forms, which are receptor-mediated glutamate excitotoxicity and non-receptor mediated glutamate oxidative toxicity. The receptor-mediated glutamate excitotoxicity involved excessive stimulation of glutamate receptors (GluRs) which lead to excessive ion calcium (Ca2+) influx and activates a cell death cascade involving the accumulation of mitochondrially generated reactive oxygen species (ROS). Studies showed excessive extracellular glutamate leads to nerve cell death via the activation of N-methyl-Daspartate (NMDA) receptors in the cases of trauma or stroke. Whereas non-receptor mediated oxidative toxicity involved the breakdown of the cystine/glutamate antiporter (xc - ) mechanism, which leads to the depletion of glutathione (GSH) and causes oxidative stress and cell death. The cystine/glutamate antiporter couples the import of cystine to the export of glutamate. The increased concentration of extracellular glutamate could inhibit the uptake of cystine, which is required for the synthesis of the intracellular antioxidant GSH. GSH plays an important role in the disposal of peroxides by brain cells and in the protection against ROS. Depletion of GSH renders the cell to oxidative stress and ultimately leading to cell death. This article aims to provide a comprehensive review of neurodegenerative diseases and the role of neurotoxin agents, glutamate in these diseases.

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“…A further supplement of antioxidants is necessary to alleviate high levels of ROS production in order to prevent deleterious effects on the cells, tissues, and organs which are associated with diseases such as cancer, diabetes, and cardiovascular and neurodegenerative diseases [2]. Other antioxidants are derived from food sources [3,4], plants [5][6][7][8], and microbes [9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activities of Streptomyces sp. strain MUSC 14 from Mangrove Forest Soil in Malaysia

Kemung

Tan

Chan

et al. 2020

BioMed Research International

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The mangrove ecosystem of Malaysia remains yet to be fully explored for potential microbes that produce biologically active metabolites. In the present study, a mangrove-derived Streptomyces sp. strain MUSC 14 previously isolated from the state of Pahang, Malaysia Peninsula, was studied for its potential in producing antioxidant metabolites. The identity of Streptomyces sp. strain MUSC14 was consistent with the genotypic and phenotypic characteristics of the Streptomyces genus. The antioxidant potential of Streptomyces sp. strain MUSC 14 was determined through screening of its methanolic extract against sets of antioxidant assays. The results were indicative of Streptomyces sp. strain MUSC 14 displaying strong antioxidant activity against ABTS, DPPH free radicals and metal chelating activity of 62.71 ± 3.30%, 24.71 ± 2.22%, and 55.82 ± 2.35%, respectively. The result of ferric reducing activity measured in terms of dose was equivalent to 2.35–2.45 μg of positive control ascorbic acid. Furthermore, there was a high correlation between the total phenolic content and the antioxidant activities with r = 0.979, r = 0.858, and r = 0.983 representing ABTS, DPPH, and metal chelation, respectively. Overall, the present study suggests that Streptomyces sp. strain MUSC 14 from mangrove forest soil has potential to produce antioxidant metabolites that can be further exploited for therapeutic application.

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Streptomyces sp. MUM273b: A mangrove‐derived potential source for antioxidant and UVB radiation protectants

Cited by 35 publications

References 108 publications

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

Streptomyces sp. Strain MUSC 125 from Mangrove Soil in Malaysia with Anti-MRSA, Anti-Biofilm and Antioxidant Activities

Comprehensive Insight of Neurodegenerative Diseases and The Role of Neurotoxin Agents — Glutamate

Antioxidant Activities of Streptomyces sp. strain MUSC 14 from Mangrove Forest Soil in Malaysia

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