sup-9, sup-10, andunc-93May Encode Components of a Two-Pore K+Channel that Coordinates Muscle Contraction inCaenorhabditis elegans

Cruz, Ignacio Perez de la; Levin, Joshua Z.; Cummins, Claudia M.; Anderson, Philip; Horvitz, H. Robert

doi:10.1523/jneurosci.23-27-09133.2003

Cited by 69 publications

(95 citation statements)

References 55 publications

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“…acr-2, unc-5 ) [14,15] (Additional file 14) whereas, a lower threshold of 1.5× included significantly more false positives (e.g. muscle genes, pat-3, sup-10 ) [16,17]. …”

Section: Resultsmentioning

confidence: 99%

A gene expression fingerprint of C. elegans embryonic motor neurons

et al. 2005

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Background: Differential gene expression specifies the highly diverse cell types that constitute the nervous system. With its sequenced genome and simple, well-defined neuroanatomy, the nematode C. elegans is a useful model system in which to correlate gene expression with neuron identity. The UNC-4 transcription factor is expressed in thirteen embryonic motor neurons where it specifies axonal morphology and synaptic function. These cells can be marked with an unc-4::GFP reporter transgene. Here we describe a powerful strategy, Micro-Array Profiling of C. elegans cells (MAPCeL), and confirm that this approach provides a comprehensive gene expression profile of unc-4::GFP motor neurons in vivo.

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“…acr-2, unc-5 ) [14,15] (Additional file 14) whereas, a lower threshold of 1.5× included significantly more false positives (e.g. muscle genes, pat-3, sup-10 ) [16,17]. …”

Section: Resultsmentioning

confidence: 99%

A gene expression fingerprint of C. elegans embryonic motor neurons

et al. 2005

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“…This hypothesis was further corroborated by the finding that a reduction in the general excitability of body wall muscle cells can also reduce polyQ aggregation. Specifically, we observed that a gain-of-function mutation in unc-93, a gene encoding a subunit of the K + channel complex that is known to reduce muscle contraction (de la Cruz et al 2003), decreased polyQ aggregation in body wall muscle cells (Supplementary Fig. S4B).…”

Section: Cholinergic Hyperstimulation Causes Premature Polyq Aggregatmentioning

confidence: 98%

Neuronal signaling modulates protein homeostasis in Caenorhabditis elegans post-synaptic muscle cells

Garcia¹,

Casanueva²,

Silva³

et al. 2007

Genes Dev.

103

133

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Protein homeostasis maintains proper intracellular balance by promoting protein folding and clearance mechanisms while minimizing the stress caused by the accumulation of misfolded and damaged proteins. Chronic expression of aggregation-prone proteins is deleterious to the cell and has been linked to a wide range of conformational disorders. The molecular response to misfolded proteins is highly conserved and generally studied as a cell-autonomous process. Here, we provide evidence that neuronal signaling is an important modulator of protein homeostasis in post-synaptic muscle cells. In a forward genetic screen in Caenorhabditis elegans for enhancers of polyglutamine aggregation in muscle cells, we identified unc-30, a neuron-specific transcription factor that regulates the synthesis of the inhibitory neurotransmitter ␥-aminobutyric acid (GABA). We used additional sensors of protein conformational states to show that defective GABA signaling or increased acetylcholine (ACh) signaling causes a general imbalance in protein homeostasis in post-synaptic muscle cells. Moreover, exposure to GABA antagonists or ACh agonists has a similar effect, which reveals that toxins that act at the neuromuscular junction are potent modifiers of protein conformational disorders. These results demonstrate the importance of intercellular communication in intracellular homeostasis.[Keywords: Protein misfolding; polyglutamine aggregation; GABAergic neurons; cholinergic neurons; protein polymorphisms] Supplemental material is available at http://www.genesdev.org.

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“…5; Supplementary Movie Fig. S3) encoding an evolutionarily conserved component of a two-pore potassium channel complex and known to be critically required for muscle contraction in Caenorhabditis elegans (de la Cruz et al 2003). The Drosophila CG8698 gene is specifically expressed in differentiating somatic muscles starting from stage 14, and its transcripts are particularly abundant in late embryonic stages in functional, contracting muscles (Fig.…”

Section: Functional Properties Of Muscle and Heart Cells Are Controllmentioning

confidence: 99%

Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors

Junion¹,

Bataillé²,

Jagla³

et al. 2007

Genes Dev.

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Correct diversification of cell types during development ensures the formation of functional organs. The evolutionarily conserved homeobox genes from ladybird/Lbx family were found to act as cell identity genes in a number of embryonic tissues. A prior genetic analysis showed that during Drosophila muscle and heart development ladybird is required for the specification of a subset of muscular and cardiac precursors. To learn how ladybird genes exert their cell identity functions we performed muscle and heart-targeted genome-wide transcriptional profiling and a chromatin immunoprecipitation (ChIP)-on-chip search for direct Ladybird targets. Our data reveal that ladybird not only contributes to the combinatorial code of transcription factors specifying the identity of muscle and cardiac precursors, but also regulates a large number of genes involved in setting cell shape, adhesion, and motility. Among direct ladybird targets, we identified bric-a-brac 2 gene as a new component of identity code and inflated encoding ␣PS2-integrin playing a pivotal role in cell-cell interactions. Unexpectedly, ladybird also contributes to the regulation of terminal differentiation genes encoding structural muscle proteins or contributing to muscle contractility. Thus, the identity gene-governed diversification of cell types is a multistep process involving the transcriptional control of genes determining both morphological and functional properties of cells.[Keywords: Cell fate; microarray; ChEST; Drosophila; ladybird; muscle; heart] Supplemental material is available at http://www.genesdev.org.

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sup-9, sup-10, andunc-93May Encode Components of a Two-Pore K⁺Channel that Coordinates Muscle Contraction inCaenorhabditis elegans

Cited by 69 publications

References 55 publications

A gene expression fingerprint of C. elegans embryonic motor neurons

A gene expression fingerprint of C. elegans embryonic motor neurons

Neuronal signaling modulates protein homeostasis in Caenorhabditis elegans post-synaptic muscle cells

Genome-wide view of cell fate specification: ladybird acts at multiple levels during diversification of muscle and heart precursors

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