TaqI digestion reveals fractions of satellite DNAs on human chromosomes

Tagarro, I.; Jj, González-Aguilera; Am, Fernández-Peralta

doi:10.1139/g91-039

Cited by 9 publications

(3 citation statements)

References 19 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Other slides were treated with different restriction enzymes (AluI, DdeI, HaeIII, RsaI, Hinf I, BamHI, and EcoRI) by the procedure described by Mezzanotte et al (1983) and Miller et al (1983). For restriction endonuclease TaqI we used the procedures described by Marchi and Mezzanotte (1988) and Tagarro et al (1991): the enzyme was dissolved in the assay buffer specified by the supplier and the preparations were incubated overnight in a moist chamber at 60°C and 37°C, respectively. Control slides were treated without enzymes.…”

Section: Methodsmentioning

confidence: 99%

Characterization of the heterochromatic chromosome regions in sheep.

et al. 1998

View full text Add to dashboard Cite

In order to elucidate the structural chromosome organization of the heterochromatic regions in sheep, we have used C-banding, silver-staining, sequential CDD technique and restriction endonuclease banding. By these banding techniques we obtained four fractions of repetitive DNA, the autosomal fractions A and B, the C fraction in the X chromosome, and the D fraction in the Y chromosome. Silver staining revealed active nucleolus organizer regions (NOR's) on the telomeric GC-rich areas of chromosomes 1, 2, 3, 4, and 25 which were digested with HaeIII restriction endonuclease.

show abstract

Section: Methodsmentioning

confidence: 99%

Characterization of the heterochromatic chromosome regions in sheep.

et al. 1998

View full text Add to dashboard Cite

show abstract

“…1977). However, the exten sive digestion of these regions by Plel and Tfil, as well as by other enzymes (Miller et al, 1983;Tagarro et al, 1991). seems to support the notion that chromatin conformation does not play a significant role in limiting the effect of these enzymes.…”

Section: Discussionmentioning

confidence: 84%

Distribution of satellite DNA fractions within major heterochromatic regions of human chromosomes as revealed by PleI and TfiI digestion

Tagarro¹,

Fernández-Peralta²,

González-Aguilera³

1992

Cytogenet Genome Res

Self Cite

View full text Add to dashboard Cite

Banding patterns induced by selective DNA extraction with the restriction endonucleases PleI and TfiI reveal the distribution of human satellite DNAs within the major heterochromatic blocks on human metaphase chromosomes. PleI and TfiI are able to discriminate HinfI target sites, depending on the nature of the central base. PleI digestion specifically reveals regions, within major C-bands, that include the major sites of satellite II DNA and permits more precise localization of satellite II domains than does radioactive in situ hybridization. The close correspondence between the cytogenetic results presented here and previously reported molecular data seems to support the idea that the frequency of enzyme target sequences is the main factor in determining the action produced by restriction endonucleases on fixed human chromosomes and that chromatin conformation is not an important factor in limiting enzyme accessibility.

show abstract

“…Low frequency of sites would then correspond with darkly stained regions, and conversely relatively high frequency of sites would give rise to poorly stained gap regions (e.g. Taql digestion of Satellite III, Tagarro et al, 1991). There is counter evidence however to suggest that other factors such 77 as access of enzymes to differentially folded chromatin and metaphase fixation methods may greater dictate the extent of digestion within a metaphase (Gonsalvez et aL, 1989;Burkholder, 1989).…”

Section: Discussionmentioning

confidence: 99%

Satellite III DNA: Regions of extreme endonuclease resistance and interindividual polymorphism in the Mb size range

Fowler

Burgoyne

1992

Genetica

View full text Add to dashboard Cite

Southern analysis of within-gel digested and restricted human cells has revealed very large Satellite III restriction fragments which show clear inter-individual length polymorphism. The Mb and sub-Mb length of these fragments indicate that they arise from regions of heterochromatin which contain homogeneous Satellite III sequences of peculiar resistance to common endonucleases. Based on sequence alone, such regions would be little digested by endonuclease digestion of chromatin in metaphase, regardless of its method of preparation. Polymorphic regions such as these might be expected to stain as part of the C-banding seen in endonuclease treated metaphase chromosomes, and may in part account for inter-individual C-band heteromorphisms.

show abstract

TaqI digestion reveals fractions of satellite DNAs on human chromosomes

Cited by 9 publications

References 19 publications

Characterization of the heterochromatic chromosome regions in sheep.

Characterization of the heterochromatic chromosome regions in sheep.

Distribution of satellite DNA fractions within major heterochromatic regions of human chromosomes as revealed by <i>Ple</i>I and <i>Tfi</i>I digestion

Satellite III DNA: Regions of extreme endonuclease resistance and interindividual polymorphism in the Mb size range

Contact Info

Product

Resources

About