<i>TaSRG</i>, a wheat transcription factor, significantly affects salt tolerance in transgenic rice and Arabidopsis

He, Xiaoliang; Hou, Xiaona; Shen, Yinzhu; Huang, Zhenyu

doi:10.1016/j.febslet.2011.03.055

Cited by 49 publications

(37 citation statements)

References 30 publications

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“…ACIP1 belongs to a small Arabidopsis protein family containing six ACIP-like isoforms (ACIP-L1 to ACIP-L6, Figure S1A). ACIP-L4 and its wheat ortholog TaSRG are required for salt tolerance [28], although their biochemical function(s) are not known. ACIP1 shares 79% identity and 87% similarity with ACIP-L1, the closest isoform.…”

Section: Resultsmentioning

confidence: 99%

AvrBsT Acetylates Arabidopsis ACIP1, a Protein that Associates with Microtubules and Is Required for Immunity

et al. 2014

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Bacterial pathogens of plant and animals share a homologous group of virulence factors, referred to as the YopJ effector family, which are translocated by the type III secretion (T3S) system into host cells during infection. Recent work indicates that some of these effectors encode acetyltransferases that suppress host immunity. The YopJ-like protein AvrBsT is known to activate effector-triggered immunity (ETI) in Arabidopsis thaliana Pi-0 plants; however, the nature of its enzymatic activity and host target(s) has remained elusive. Here we report that AvrBsT possesses acetyltransferase activity and acetylates ACIP1 (for ACETYLATED INTERACTING PROTEIN1), an unknown protein from Arabidopsis. Genetic studies revealed that Arabidopsis ACIP family members are required for both pathogen-associated molecular pattern (PAMP)-triggered immunity and AvrBsT-triggered ETI during Pseudomonas syringae pathovar tomato DC3000 (Pst DC3000) infection. Microscopy studies revealed that ACIP1 is associated with punctae on the cell cortex and some of these punctae co-localize with microtubules. These structures were dramatically altered during infection. Pst DC3000 or Pst DC3000 AvrRpt2 infection triggered the formation of numerous, small ACIP1 punctae and rods. By contrast, Pst DC3000 AvrBsT infection primarily triggered the formation of large GFP-ACIP1 aggregates, in an acetyltransferase-dependent manner. Our data reveal that members of the ACIP family are new components of the defense machinery required for anti-bacterial immunity. They also suggest that AvrBsT-dependent acetylation in planta alters ACIP1's defense function, which is linked to the activation of ETI.

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Section: Resultsmentioning

confidence: 99%

AvrBsT Acetylates Arabidopsis ACIP1, a Protein that Associates with Microtubules and Is Required for Immunity

et al. 2014

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“…A plethora of comparisons between salt-sensitive and salt-tolerant cultivars of model and non-model plant species, including Arabidopsis [20–22], rice [23], poplar [24–27], tomato [28], potato [29], Medicago truncatula [30], sugarcane [31] and olive [32], have been reported to date. These studies have identified more than 30 families of transcription factors and numerous enzyme-encoding genes involved in responses to salt stress [33, 34]. However, overall changes in gene expression and physiological responses to salt stress vary greatly between different species, particularly between sensitive and non-sensitive pairs of related species [35–39].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome differences between two sister desert poplar species under salt stress

et al. 2014

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BackgroundPopulus euphratica Oliv and P. pruinosa Schrenk (Salicaceae) both grow in dry desert areas with high summer temperatures. However, P. euphratica is distributed in dry deserts with deep underground water whereas P. pruinosa occurs in deserts in which there is underground water close to the surface. We therefore hypothesized that these two sister species may have evolved divergent regulatory and metabolic pathways during their interaction with different salt habitats and other stresses. To test this hypothesis, we compared transcriptomes from callus exposed to 24 h of salt stress and control callus samples from both species and identified differentially expressed genes (DEGs) and alternative splicing (AS) events that had occurred under salt stress.ResultsA total of 36,144 transcripts were identified and 1430 genes were found to be differentially expressed in at least one species in response to salt stress. Of these DEGs, 884 and 860 were identified in P. euphratica and P. pruinosa, respectively, while 314 DEGs were common to both species. On the basis of parametric analysis of gene set enrichment, GO enrichment in P. euphratica was found to be significantly different from that in P. pruinosa. Numerous genes involved in hormone biosynthesis, transporters and transcription factors showed clear differences between the two species in response to salt stress. We also identified 26,560 AS events which were mapped to 8380 poplar genomic loci from four libraries. GO enrichments for genes undergoing AS events in P. euphratica differed significantly from those in P. pruinosa.ConclusionsA number of salt-responsive genes in both P. euphratica and P. pruinosa were identified and candidate genes with potential roles in the salinity adaptation were proposed. Transcriptome comparisons of two sister desert poplar species under salt stress suggest that these two species may have developed different genetic pathways in order to adapt to different desert salt habitats. The DEGs that were found to be common to both species under salt stress may be especially important for future genetic improvement of cultivated poplars or other crops through transgenic approaches in order to increase tolerance of saline soil conditions.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2164-15-337) contains supplementary material, which is available to authorized users.

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“…TaSOS1 is involved in sodium exclusion at plasma membrane level in root cells and TaNHX1 and TaNHX2 at vacuolar level. Other reports indicated the role of transcription factors (TaSRG and TaRUB) under stress conditions (He et al 2011;Zhang et al 2012) but very few studies were related to differential expression of these genes (Rana et al 2015). Dubcovsky et al (1996) reported Kna1 locus mapped on chromosome 4DL involved in Na + exclusion from leaves and discrimination of K + over Na + concentration in roots.…”

Section: Introductionmentioning

confidence: 99%

Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

Rana

Ram

Nehra

et al. 2016

Cereal Research Communications

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In the present investigation, expression of genes related to Na + exclusion such as salt overly sensitive (TaSOS1) and Na + /H + antiporter (TaNHX1) and proline accumulation such as pyrroline-5-carboxylate reductase (P5CR) and glutamate synthase (GOGAT) was studied in seedlings of Kharchia 65 (Kh 65, salt tolerant) and HD 2009 (sensitive) under salt stress (ECe, 12 dSm -1 ) and controlled conditions. As compared to HD 2009, Kh 65 showed significantly lower accumulation of Na + (p < 0.01) and higher accumulation of proline (p < 0.05) in leaf blade under salt stress. The relative expression of TaSOS1 increased significantly (p < 0.001) in roots of Kh 65 (4.31-fold) while it decreased in HD 2009. There was significantly higher (p < 0.01) relative expression of TaNHX1 (27.57-fold) in leaf and 3.07-fold in roots of Kh 65 as compared to 3.65-and 0.87-fold increase in leaf and roots of HD 2009, respectively, under salt stress. There was significantly (p < 0.05) higher accumulation of proline as compared to HD 2009 in leaf tissues. There was significantly higher (p < 0.01) expression of P5CR (5.23-fold in leaf and 8.77-fold in the root) and glutamate synthase (6.0-fold in roots) in Kh 65 as compared to HD 2009. The study demonstrated that upregulation of genes for Na + exclusion in root and compartmentation in leaf and increased proline concentration are associated with tolerance to salinity stress in wheat. The information will be useful for improving wheat genotypes for salt tolerance.

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TaSRG, a wheat transcription factor, significantly affects salt tolerance in transgenic rice and Arabidopsis

Cited by 49 publications

References 30 publications

AvrBsT Acetylates Arabidopsis ACIP1, a Protein that Associates with Microtubules and Is Required for Immunity

AvrBsT Acetylates Arabidopsis ACIP1, a Protein that Associates with Microtubules and Is Required for Immunity

Transcriptome differences between two sister desert poplar species under salt stress

Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

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TaSRG, a wheat transcription factor, significantly affects salt tolerance in transgenic rice and Arabidopsis

Cited by 49 publications

References 30 publications

AvrBsT Acetylates Arabidopsis ACIP1, a Protein that Associates with Microtubules and Is Required for Immunity

AvrBsT Acetylates Arabidopsis ACIP1, a Protein that Associates with Microtubules and Is Required for Immunity

Transcriptome differences between two sister desert poplar species under salt stress

Expression of genes related to Na+exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress

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Expression of genes related to Na⁺exclusion and proline accumulation in tolerant and susceptible wheat genotypes under salt stress