Marine aggregates are naturally forming conglomerations of larvacean houses, phytoplankton, microbes, and inorganics adhered together by exocellular polymers. In this study, we show in vitro that the bacterial pathogen Vibrio vulnificus can be concentrated into laboratory-generated aggregates from surrounding water. We further show that environmental (E-genotype) strains exhibit significantly more integration into these aggregates than clinical (C-genotype) strains. Experiments where marine aggregates with attached V. vulnificus cells were fed to oysters (Crassostrea virginica) resulted in greater uptake of both C and E types than nonaggregated controls. When C-and E-genotype strains were cocultured in competitive experiments, the aggregated E-genotype strains exhibited significantly greater uptake by oyster than the C-genotype strains.
Vibrio vulnificus is a Gram-negative, halophilic bacterium capable of causing gastroenteritis, wound infections, and fatal septicemia in humans (1-3). It is routinely found in waters of estuarine environments as part of the normal microflora, as well as in oysters and other shellfish inhabiting those estuaries (3). V. vulnificus infection is the leading cause of seafood-borne deaths in the United States, usually resulting from the consumption of raw or undercooked oysters (3). Infections caused by ingestion commonly result in primary septicemia, almost always require hospitalization, and have a fatality rate of greater than 50% (3, 4). Wound infections usually result from exposure of open wounds to seawater containing the bacterium and can progress to fatal necrotizing fasciitis (5, 6).V. vulnificus exhibits high genotypic and phenotypic variation (3) and is divided into two genotypes, a difference originally discovered by randomly amplified polymorphic DNA PCR analysis of strains from both clinical and environmental sources (7). In this classification system, a gene designated vcg (virulence-correlated gene) was found to have two variations (8). One allele (vcgC) correlates highly with strains obtained from clinical sources and is designated the C genotype, while the other (vcgE) is correlated with environmentally isolated strains and is designated the E genotype (7,8).Over 95% of infections resulting in septicemia caused by V. vulnificus involve the consumption of raw oysters, with the remainder arising from ingestion of steamed oysters and clams (3). While millions of people in the United States eat raw oysters (9), if a consumer is afflicted with a predisposing condition, such as liver impairment or immune system dysfunction (10), the risk for infection increases 80-fold (11). Even considering these two facts, it is surprising that there are only ca. 40 primary septicemia cases reported per year (10). Usually, oysters predominately contain the E-genotype strains of V. vulnificus, which is likely a factor in the low number of infections (12-18).A study comparing the population dynamics of the V. vulnificus genotypes revealed an interesting phenomenon. While Warner and Oliver (...