Tinca Gobba Dorata del Pianalto di Poirino: genetic characterization by microsatellite markers

Abstract

The Tinca Gobba Dorata del Pianalto di Poirino (Golden humped tench of Poirino highland, [PO] 0.29 to 0.40). The analysis of between-population differentiation indicated that PO significantly differed from the others (FST = 0.039 to 0.097, P<0.05), while BO and TR were the most similar, consistently with their geographic proximity. The Neighbour-Joining tree revealed a clear separation between Northern and Central populations, with a bootstrap support of 97%. The population differentiation was reflected by the results of the assignment test, with 64% to 92% of the individuals correctly assigned to the original population, and a probability ranging from 76% to 95%. No individuals belonging to other populations were erroneously assigned to PO. A more detailed analysis of the PO population showed a similar genetic variability within the 15 considered ponds and a low degree of differentiation between ponds, with the exception of one "historical" pond, which significantly differed from most of the others, thus deserving to be preserved. The results indicate that the PO, despite being farmed, has a high level of withinpopulation diversity and is greatly differentiated from the other populations considered. The possibility of applying the assignment test in the framework of the product traceability deserves further investigation.

Genetic variability in tench (Tinca tincaL.) as revealed by PCR-RFLP analysis of mitochondrial DNA

“…However, the mtDNA markers generally revealed a lower power than microsatellite markers in detecting the within-population variability. In fact, the average haplotype diversity level of mtDNA (Hmt) observed in the present study (0.215) was lower than the average heterozygosity level of microsatellites (Hms) deduced from the data of Kohlmann et al (2010) andLo Presti et al (2010b) on the same populations (0.343). However, in some populations in which mtDNA was polymorphic, Hmt was even higher than Hms (TUR, FEL, ALC, TRA, BOL).…”

“…Data on the 5 Italian populations previously studied for the same mtDNA segments (Lo Presti et al, 2010a) were also included in order to cover a larger geographical area (Table 1). All the populations had been already analyzed by microsatellite markers Lo Presti et al, 2010b).…”

“…In fact, apart from some studies carried out over the past decades by means of protein markers (Valenta et al, 1978;Šlechtova et al, 1995;Kohlmann and Kersten, 1998), only recently have tench specific microsatellite loci been described (Kohlmann and Kersten, 2006) and used to characterize many European and Asian populations Lo Presti et al, 2010b). Also, the complete mitochondrial DNA (mtDNA) sequence has only recently been made available (Saitoh et al, 2006).…”

Integrated pond aquaculture and regional identity: ethnobiology of the golden humped tench of Poirino highlands, Northwest Italy

“…The exchange and gift of fries and tenches among the inhabitants of Poirino highlands is an important practice that reproduces the system and tightens the community, rooting a collective identity into the region and its gastronomic manifestations. The continuous exchange and movement of fries between ponds may also underlie the high level of genetic diversity among golden humped tenches and within ponds as reported by Presti et al [ 47 ].…”

Distribution of five growth hormone gene haplogroups in wild and cultured tench, Tinca tinca L., populations

“…On the other hand, current attempts to diversify pond fish production have also led to an increased interest in tench. Considerable genetic differentiation among tench populations (Kohlmann et al., ; Lo Presti et al., , ; Lajbner et al., ) as well as differences in the growth performance of tench strains (Gela et al., ; Kocour et al., ) has been documented. Thus, genetic improvement of the tench growth rate seems feasible.…”