trans-10,cis-12 Conjugated linoleic acid specifically increases tissue α-tocopherol mediated by PPARγ inhibition in mice

Abstract

C57BL/6J mice were divided into control group (C), CLA, c9t11, or t10c12 groups. CLA and t10c12 significantly increased α-tocopherol levels in the plasma and various tissues in experiment 1. The CLA and t10c12 groups also showed a significant increase in hepatic α-tocopherol transfer protein (α-TTP) levels. In experiment 2, mice were divided into control, CLA, R (rosiglitazone, a PPARγ agonist), or CLA+R groups. Vitamin E levels in the liver, epididymal fat pad, kidney, and plasma were increased by CLA, and this effect was reduced in the CLA+R group. t10,c12-CLA is the most active isomer in the CLA mixture in the regulation of tissue vitamin E status and α-TTP protein levels in mice. The increase in liver vitamin E status in CLA-fed mice is mainly due to the effect of PPARγ inhibition.

Conjugated Linoleic Acid and Postmenopausal Women's Health

“…These isomers have shown to exhibit additive, independent, or antagonistic effects (Park and Pariza ). It has been demonstrated that the cis ‐9, trans ‐11 CLA isomer is responsible for anti‐inflammatory effect and inhibition of hepatic sterol regulatory element binding protein‐1c expression (Roche and others ; Moloney and others ), whereas the trans ‐10, cis ‐12 isomer is responsible for body fat reduction, enhancing endurance capacity, increases in tissue α‐tocopherol, and reduction of hepatic apolipoprotein B secretion (Park and others ; Storkson and others ; Kim and others ; Wang and others ).…”