<i>Trans</i>‐11 vaccenic acid improves insulin secretion in models of type 2 diabetes in vivo and in vitro

Gupta, Joel; Kerslake, Matthew; Rayat, Gina R.; Proctor, Spencer D.; Chan, Catherine B.

doi:10.1002/mnfr.201500783

Cited by 29 publications

(18 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Chronic TFA intake was suggested to be associated with the risk of type 2 diabetes [19] against diabetes [31,32]. In spite of the evident need for better insight and understanding, the putative toxicity of TFAs has been scarcely investigated at cellular and molecular levels.…”

Section: Discussionmentioning

confidence: 99%

Cellular toxicity of dietary trans fatty acids and its correlation with ceramide and diglyceride accumulation

Sarnyai

Donkó

Mátyási³

et al. 2019

Food and Chemical Toxicology

View full text Add to dashboard Cite

Highlights  Cellular metabolism and toxicity of TFAs are still to be elucidated.  TFAs were incorporated in RINm5F insulinoma cells like palmitate or oleate.  Similarly to oleate and unlike palmitate, TFAs were of mild toxicity.  FA-induced cell damage correlated with ceramide and diglyceride accumulation.  Incorporation of TFAs in ceramides and diglycerides exceeded that of oleate.

show abstract

Section: Discussionmentioning

confidence: 99%

Cellular toxicity of dietary trans fatty acids and its correlation with ceramide and diglyceride accumulation

Sarnyai

Donkó

Mátyási³

et al. 2019

Food and Chemical Toxicology

View full text Add to dashboard Cite

show abstract

“…It was reported recently that a higher concentration of cis -VA in plasma was associated with a reduced risk of T2DM, lower fasting glucose and better insulin sensitivity in male Sprague–Dawley rat models [ 31 ], and in T2DM middle-aged and elderly Chinese patients [ 32 ]. Chen et al studied circulating (serum) fatty acids and detected impaired composition of fatty acids not only in women with GDM, but also in women with less severe glucose intolerance.…”

Section: Discussionmentioning

confidence: 99%

Lipid profile changes in erythrocyte membranes of women with diagnosed GDM

et al. 2018

View full text Add to dashboard Cite

Gestational diabetes mellitus (GDM) is a glucose intolerance that begins or is first recognized during pregnancy. It is currently a growing health problem worldwide affecting from 1% to 14% of all pregnant women depending on racial and ethnic group as well as the diagnostic and screening criteria. Our preliminary study aimed at investigating the erythrocyte membrane fatty acid profiles of pregnant women, in particular with diagnosed with gestational diabetes mellitus (GDM), and with normal glucose tolerant (NGT) pregnant women as a control group. The study group comprised 43 pregnant women, 32 of whom were diagnosed with GDM according to the WHO criteria, and 11 with normal glucose tolerance. The erythrocyte membrane phospholipids were obtained according to the Folch extraction procedure. Fatty acids (FA) were analyzed by gas chromatography (GC) as the corresponding fatty acid methyl esters (FAME). A cluster of 14 fatty acids identified contained >98% of the recognized peaks in the GC analysis. The analysis of fatty acids from erythrocytes revealed important differences between GDM and NGT women in the third trimester, and the results were correlated with biochemical data. Among the 14 measured FA representing the membrane lipidomic profile, the levels of three saturated FA (myristic, palmitic, stearic acids) tended to decrease in GDM patients, with the percentage content of stearic acid significantly changed. The relative content of monounsaturated fatty acids (MUFA) tended to increase, in particular the oleic acid and vaccenic acid contents were significantly increased in erythrocyte membranes of the GDM group in comparison with the NGT group. The GDM group demonstrated higher sapienic acid levels (+29%) but this change was not statistically significant. This study revealed association between an impaired cis-vaccenic acid concentration in erythrocytes membrane and GDM development. No significant changes of polyunsaturated fatty acids (PUFA) were observed in GDM and NGT erythrocytes. We postulate, basing on the differences between the GDM and NGT lipidomic profiles, that stearic and cis-vaccenic acids can be considered as dual biomarkers of specific SFA-MUFA conversion pathway, involving the coupling of delta-9 desaturase and elongase enzymes. Our results indicate that the SFA-MUFA families may be involved in the pathophysiology of metabolic diseases such as GDM, but the further studies are needed to confirm our hypothesis. In conclusion, the erythrocyte membranes of GDM women undergo remodeling resulting in abnormal fatty acid profiles, which are reflection of the long-term status of organism and can have great impact on both the mother and her offspring.

show abstract

“…Fortunately, some nutritional studies involving chemically synthesized TVA were carried out either on humans [ 20,21 ] or rodents. [ 22–26 ] Therefore, any methodology used for getting high amounts of pure TVA might be translated to TPA. To the best of our knowledge, two ways were used to obtain TVA.…”

Section: Fatty Acid Macadamia Nut Oil [%] Provinal [%]mentioning

confidence: 99%

“…The first one consists of alkali isomerization of vegetable oils. [ 22–26 ] According to the second way, cis ‐vaccenic acid ( cis ‐11 C18:1, or C18:1 n‐7 cis , CVA) is chemically synthesized first, then a cis / trans isomerization and a cis / trans fractionation are carried out. [ 20,21,27–31 ]…”

Section: Fatty Acid Macadamia Nut Oil [%] Provinal [%]mentioning

confidence: 99%

“…[ 27 ] As a result, previous research relied on Wittig reaction to get CVA ready for isomerization to TVA. [ 21–28 ] Translating to cis ‐C16:1 fatty acids and assuming that convenient reagents are commercially available, Wittig reaction can be performed to get high amounts of pure CPA. However, CPA is a dietary fatty acid that does occur in several foods in significant amounts.…”

Section: Fatty Acid Macadamia Nut Oil [%] Provinal [%]mentioning

confidence: 99%

See 1 more Smart Citation

Chemical Synthesis and Isolation of Trans‐Palmitoleic Acid (Trans‐C16:1 n‐7) Suitable for Nutritional Studies

Guillocheau

Drouin

Catheline

et al. 2020

Euro J Lipid Sci & Tech

View full text Add to dashboard Cite

Trans-palmitoleic acid (trans-9 C16:1, or trans-C16:1 n-7, TPA) is typically found in ruminant-derived foods (milk and meat). Of note, previous epidemiological studies associated high levels of circulating TPA with a lower risk of type 2 diabetes and metabolic syndrome in humans. At the current time, TPA intakes in humans are ensured by ruminant-derived foods. However, due to the very low commercial availability of TPA, there are no supplementation studies carried out so far. Therefore, the ability for dietary TPA to prevent type 2 diabetes and metabolic syndrome has never been experimentally assessed. Here, a method (among others) to get dozens of grams of pure TPA as ethyl ester, to perform dedicated supplementation studies, is reported. For that purpose, it starts from food sources containing high amounts of cis-palmitoleic acid (cis-9 C16:1, or cis-C16:1 n-7, CPA), dealing with fatty acids ethyl esters all along the experiment. CPA is purified with flash liquid chromatography, then submitted to a cis/trans isomerization step. Finally, TPA is separated from CPA by low-temperature crystallization in methanol. The final product is fully characterized by 1 H and 13 C nuclear magnetic resonance spectrometry. It is possible to produce ≈70 g of 85%-purity TPA suitable for nutritional studies. Practical Applications: The synthesized trans-palmitoleic acid may serve for supplementation (or nutritional) studies aiming at unravelling its physiological impacts suggested by epidemiological work. Depending on the amount of synthesized trans-palmitoleic acid, one may carry out nutritional studies on rodents or even on humans.Trans-palmitoleic acid (trans-9 C16:1, or trans-C16:1 n-7, TPA) belongs to the family of natural trans fatty acids, as reviewed by

show abstract

Trans‐11 vaccenic acid improves insulin secretion in models of type 2 diabetes in vivo and in vitro

Abstract: These results indicate that VA may improve insulin secretion and growth of islets in T2D, at least partly by altering GPR40 and REG-1α mRNA expression.

Cited by 29 publications

References 52 publications

Cellular toxicity of dietary trans fatty acids and its correlation with ceramide and diglyceride accumulation

Cellular toxicity of dietary trans fatty acids and its correlation with ceramide and diglyceride accumulation

Lipid profile changes in erythrocyte membranes of women with diagnosed GDM

Chemical Synthesis and Isolation of Trans‐Palmitoleic Acid (Trans‐C16:1 n‐7) Suitable for Nutritional Studies

Contact Info

Product

Resources

About