<i>trans</i>‐Resveratrol inhibits calcium influx in thrombin‐stimulated human platelets

Dobrydneva, Yuliya; Williams, R. L.; Blackmore, Peter F.

doi:10.1038/sj.bjp.0702749

Cited by 82 publications

(53 citation statements)

References 36 publications

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“…These results suggest that SOC or IICR may be involved in the chemotaxis of the ascidian sperm. The IC 50 of 2-APB for capacitative Ca 2ϩ entry is Ϸ5 Ϫ 10 M (27,28), and that of IICR is 42 M (25). Therefore, the inhibition of sperm chemotaxis by 2-APB at the dose used in these experiments (50 M) may be mainly because of its inhibitory effect on the SOC.…”

Section: Resultsmentioning

confidence: 95%

See 1 more Smart Citation

Store-operated calcium channel regulates the chemotactic behavior of ascidian sperm

Yoshida

Ishikawa

Izumi

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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The sperm-activating and -attracting factor released from the eggs of the ascidians Ciona intestinalis and Ciona savignyi requires extracellular Ca 2؉ for activating sperm motility and eliciting chemotactic behavior of the activated sperm toward the egg. Here, we show that modulators of the store-operated Ca 2؉ channel, SK&F96365, Ni 2؉ , 2-aminoethoxydiphenylborane, and thapsigargin inhibit the chemotactic behavior of the ascidian sperm; on the other hand, blockers of voltage-dependent Ca 2؉ channels did not inhibit the chemotaxis, even though they inhibited the sperm activation operated by voltage-dependent Ca 2؉ channels. The blockers of store-operated Ca 2؉ channels also inhibited the asymmetrical flagellar beating and turning movements of the ascidian sperm, which are typical signs of sperm chemotaxis. Depletion of internal Ca 2؉ stores by thapsigargin induced capacitative Ca 2؉ entry into the sperm, which was blocked by SK&F96365. These results suggest that the intracellular Ca 2؉ concentration increase through the store-operated Ca 2؉ channels induces asymmetrical flagellar movements to establish the chemotactic behavior of the sperm. I t is now well known that many cells, e.g., leukocytes, starved amoebae of Dictyostelium discoideum, bacteria, and migrating axon cells during development, can detect an extracellular chemical gradient and migrate toward the source of the chemicals, that is, exhibit chemotactic behavior. There is a variety of factors inducing chemotaxis of cells, e.g., food, pathogens, partner cells, etc., and chemotactic behavior of cells is one of the important communication systems between separated cells. Chemotactic behavior of the sperm toward an egg during fertilization in animal species is a typical case, first reported in hydrozoans (1). This phenomenon is now widely known to occur, from sponges to humans (2-4), and much effort has been devoted to clarifying the mechanisms underlying sperm chemotaxis.Extracellular Ca 2ϩ is generally accepted as being an important factor in the induction of chemotactic behavior of sperm in many plant and animal species (5-8). In the case of the sea urchin, Arbacia punctulata, a peptide named resact in the jelly layer of the egg has been shown to have sperm-activating and -attracting activities (7). The peptide increases the intracellular Ca 2ϩ concentration ([Ca 2ϩ ] i ) via cAMP-gated Ca 2ϩ channels (9, 10), which, in turn, induces asymmetrical flagellum movements (5, 11, 12) that result in reorientation of the sperm swimming direction (13). The role of extracellular Ca 2ϩ -induced asymmetrical flagellar movement in inducing sperm chemotaxis has also been reported in hydrozoan species (14). Ascidian sperm chemotactic behavior is also characterized by abrupt turning movements of the sperm (15, 16). Furthermore, it has been shown that the sperm-attracting activity in the ascidians Ciona intestinalis and Ciona savignyi does not originate from the overall surface of the egg coats, such as follicle cells, but from the vegetal pole of the egg (17). We al...

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Section: Resultsmentioning

confidence: 95%

“…2-APB, originally characterized as a cell-permeable inhibitor of inositol 1,4,5-trisphosphate (IP 3 )-induced Ca 2ϩ release (IICR) (25), also acts as an inhibitor of SOC (26)(27)(28). To confirm the involvement of SOC in chemotaxis of the ascidian sperm, we observed the effects of 2-APB on the sperm chemotaxis of C. savignyi.…”

Section: Resultsmentioning

confidence: 99%

Store-operated calcium channel regulates the chemotactic behavior of ascidian sperm

Yoshida

Ishikawa

Izumi

et al. 2002

Proc. Natl. Acad. Sci. U.S.A.

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“…Most interestingly, this inhibitory effect appeared to consist of at least two components with different potencies, suggesting that DPTHF might inhibit TRPV3 via more than one mechanism or site of action. Although the three diphenyl-containing compounds used in this study, 2-APB, DPBA, and DPTHF, have all been shown to inhibit store-operated Ca 2ϩ influx (18), not all actions of these drugs are the same. For instance, DPTHF is ineffective at blocking volume-regulated anion channels (24).…”

Section: Resultsmentioning

confidence: 99%

“…Two structural analogs of 2-APB, diphenylboronic anhydride (DPBA) and 2,2-diphenyltetrahydrofuran (DPTHF), have also been reported to inhibit store-operated calcium entry (18). However, the activity profiles of these compounds are not identical.…”

mentioning

confidence: 85%

Biphasic Currents Evoked by Chemical or Thermal Activation of the Heat-gated Ion Channel, TRPV3

Chung¹,

Güler²,

Caterina³

2005

Journal of Biological Chemistry

140

139

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2-Aminoethyl diphenylborinate was recently identified as a chemical activator of TRPV1, TRPV2, and TRPV3, three heat-gated members of the transient receptor potential vanilloid (TRPV) ion channel subfamily. Here we demonstrated that two structurally related compounds, diphenylboronic anhydride (DPBA) and diphenyltetrahydrofuran (DPTHF), can also modulate the activity of these channels. DPBA acted as a TRPV3 agonist, whereas DPTHF exhibited prominent antagonistic activity. However, all three diphenyl-containing compounds promoted some degree of channel activation or potentiation, followed by channel block. Strong TRPV3 activation by DPBA often leads to the appearance of a secondary, enhanced, current phase. A similar biphasic response was observed during TRPV3 heat stimulation; an initial, gradually sensitizing phase (I 1 ) was followed by an abrupt transition to a secondary phase (I 2 ). I 2 was characterized by larger current amplitude, loss of outward rectification, and alterations in the following properties: permeability among cations; ruthenium red and DPTHF sensitivity; temperature dependence; and voltage-dependent gating. The I 1 to I 2 transition depended strongly on TRPV3 current density. Removal of extracellular divalent cations resulted in heat-evoked currents resembling I 2 , whereas mutation of a putative Ca 2؉ -binding residue in the pore loop domain, aspartate 641, facilitated detection of the I 1 to I 2 transition, suggesting that the conversion to I 2 resulted from the agonist-and time-dependent loss of divalent cationic inhibition. Primary keratinocytes overexpressing exogenous TRPV3 also exhibited biphasic agonist-evoked currents. Thus, strong activation by either chemical or thermal stimuli led to biphasic TRPV3 signaling behavior that may be associated with changes in the channel pore.

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“…The cells were then washed and resuspended in calcium-free or calcium-containing buffer at a concentration of 7.7 ϫ 10 6 cells/ml. Calcium measurements were performed in a SPEX ARCM spectrofluorometer, similar to that described previously (32). HL-60 cells were first placed in the fluorometer cuvette, to obtain a base-line reading, and cells were then removed from the fluorometer cuvette with a Pasteur pipette and added to the BioSmith cuvette.…”

Section: Fluorometric Analysis Of Internal Calciummentioning

confidence: 99%

Stimulation of Capacitative Calcium Entry in HL-60 Cells by Nanosecond Pulsed Electric Fields

White

Blackmore

Schoenbach

et al. 2004

Journal of Biological Chemistry

225

157

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Nanosecond pulsed electric fields (nsPEFs) are hypothesized to affect intracellular structures in living cells providing a new means to modulate cell signal transduction mechanisms. The effects of nsPEFs on the release of internal calcium and activation of calcium influx in HL-60 cells were investigated by using real time fluorescent microscopy with Fluo-3 and fluorometry with Fura-2. nsPEFs induced an increase in intracellular calcium levels that was seen in all cells. With pulses of 60 ns duration and electric fields between 4 and 15 kV/cm, intracellular calcium increased 200 -700 nM, respectively, above basal levels (ϳ100 nM), while the uptake of propidium iodide was absent. This suggests that increases in intracellular calcium were not because of plasma membrane electroporation. nsPEF and the purinergic agonist UTP induced calcium mobilization in the presence and absence of extracellular calcium with similar kinetics and appeared to target the same inositol 1,4,5-trisphosphate-and thapsigargin-sensitive calcium pools in the endoplasmic reticulum. For cells exposed to either nsPEF or UTP in the absence of extracellular calcium, there was an electric field-dependent or UTP dose-dependent increase in capacitative calcium entry when calcium was added to the extracellular media. These findings suggest that nsPEFs, like ligand-mediated responses, release calcium from similar internal calcium pools and thus activate plasma membrane calcium influx channels or capacitative calcium entry.

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trans‐Resveratrol inhibits calcium influx in thrombin‐stimulated human platelets

Cited by 82 publications

References 36 publications

Store-operated calcium channel regulates the chemotactic behavior of ascidian sperm

Store-operated calcium channel regulates the chemotactic behavior of ascidian sperm

Biphasic Currents Evoked by Chemical or Thermal Activation of the Heat-gated Ion Channel, TRPV3

Stimulation of Capacitative Calcium Entry in HL-60 Cells by Nanosecond Pulsed Electric Fields

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