trans-Trismethoxy resveratrol decreased fat accumulation dependent on fat-6 and fat-7 in Caenorhabditis elegans

Yue, Yiren; Shen, Peiyi; Chang, Amanda; Qi, Weipeng; Kim, Kee‐Hong; Kim, Dae-Young; Park, Yeonhwa

doi:10.1039/c9fo00778d

Cited by 28 publications

(57 citation statements)

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“…TUB-1, an ortholog of the mammalian TUBBY, is associated with the regulation of lipid metabolism via a neuroendocrine pathway in C. elegans ( Yue et al., 2019 ). Isorhamnetin at 200 μM decreased triglyceride content in tub-1 mutant by 46%, compared to the control ( Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Human lipid metabolism pathways are conserved in C. elegans , which help to identify the mechanisms of action of food components prior to vertebrate animals and/or humans ( Shen et al., 2018a , Shen et al., 2018b ). Previously, studies have examined the anti-obesity effects of plant extracts and food bioactive components in C. elegans , showing the relevance of this animal model for the mechanistic studies of prospective bioactives ( Bhattacharya et al., 2013 , Farias-Pereira et al., 2018 , Farias-Pereira et al., 2020 , Lin et al., 2019 , Liu et al., 2018 , Machado et al., 2018 , Shen et al., 2017 , Shen et al., 2018a , Shen et al., 2018b , Shen et al., 2018c , Sun et al., 2016 , Yue et al., 2019 ). Thus, the goal of this study was to determine the effects of isorhamnetin on lipid metabolism by using C. elegans as an animal model.…”

Section: Introductionmentioning

confidence: 99%

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Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Farias-Pereira

Savarese

Yue

et al. 2020

Current Research in Food Science

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Isorhamnetin (3- O -methylquercetin), a flavonol found in dill weed, sea buckthorn berries, kale and onions, has been suggested to have anti-obesity effects, but there is limited evidence of its mechanisms of action on lipid metabolism. The goal of this study was to investigate the effects of isorhamnetin on lipid metabolism using Caenorhabditis elegans as an animal model. Isorhamnetin reduced fat accumulation without affecting food intake or energy expenditure in C. elegans . The isorhamnetin's fat-lowering effects were dependent on nhr-49 , a homolog of the human peroxisome proliferator-activated receptor alpha (PPARα). Isorhamnetin upregulated an enoyl-CoA hydratase ( ech-1.1 , involved in fatty acid β-oxidation) and adipose triglyceride lipase ( atgl-1 , involved in lipolysis) via NHR-49-dependent pathway at transcriptional levels. Isorhamnetin also upregulated the C. elegans AMP-activated protein kinase (AMPK) subunits homologs ( aak-1 and aak-2 ), involved in energy homeostasis. These results suggest that isorhamnetin reduces body fat by increasing fat oxidation in part via NHR-49/PPARα-dependent pathway.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Farias-Pereira

Savarese

Yue

et al. 2020

Current Research in Food Science

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“…28 In C. elegans, the deletion of tub-1 (the homolog of the mammalian Tubby) causes an increased fat accumulation phenotype, which may be due to changes on lipid metabolism. 19,20,28 Our results showed that butein did not regulate tub-1 expression in wild-type C. elegans (Figure 2a). Moreover, butein reduced triglyceride content (p = .0095) compared to the control in tub-1 mutant (Figure 3c), suggesting that tub-1 is not a genetic requirement for the reduced triglyceride content by butein in C. elegans.…”

Section: The Effects Of Butein On Lipid Metabolism-related Transcrimentioning

confidence: 74%

“…Identified fatty acids were measured as the area (%) of total fatty acids, then the desaturation index was determined by quantifying the ratio 18:1Δ9/18:0. 20…”

Section: Fatty Acid Analysismentioning

confidence: 99%

“…The fatty acid Δ9 desaturases are known targets for antiobesity agents due to the inhibition of fatty acid desaturation, which may lead to a reduced fat accumulation. 20,[29][30][31][32][33] In C. elegans, palmitoyl-CoA desaturase (fat-5) is responsible for the desaturation of palmitic acid (16:0) into palmitoleic acid (16:1), while the human stearoyl-CoA desaturase (SCD) homologs (fat-6 and fat-7) converts stearic acid (18:0) into oleic acid (18:1Δ9). 20,29 Butein did not influence fat-5 and fat-6 expressions, however, butein at 35 and 70 μM downregulated the fat-7 expression by 31 and 37% (p = .0249 and .0060, respectively) compared to the control (Figure 2b).…”

Section: The Effects Of Butein On Lipid Metabolism-related Enzymesmentioning

confidence: 99%

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Butein inhibits lipogenesis in Caenorhabditis elegans

et al. 2020

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Butein, a flavonoid found in annatto seeds and lacquer trees, may be used for many health benefits, including the prevention of obesity. However, its antiobesity effects are not completely understood; in particular, the effects of butein on the regulation of lipid metabolism have not been explained. Thus, the goal of the current study was to determine the effects of butein on lipid metabolism in Caenorhabditis elegans, which is a multi-organ nematode used as an animal model in obesity research. Butein at 70 μM reduced triglyceride content by 27% compared to the control without altering food intake and energy expenditure. The reduced triglyceride content by butein was associated with the downregulation of sbp-1, fasn-1, and fat-7, the lipogenesis-related homologs of sterol regulatory element-binding proteins, fatty acid synthase and stearoyl-CoA desaturase, respectively. Furthermore, fat-7 and skn-1, a homolog of nuclear respiratory factors, were identified as genetic requirements for butein's effects on triglyceride content in C. elegans. The effects of butein on sbp-1 and fasn-1 were dependent on skn-1, but the downregulation of fat-7 was independent of skn-1. These results suggest that the inhibitory effects of butein on lipogenesis are via SKN-1-and FAT-7-dependent pathways in C. elegans.

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Application of Caenorhabditis elegans in the evaluation of food nutrition: A review

Xiao

et al. 2023

eFood

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At present, more and more people are suffering from various diseases and it is particularly important to find a suitable biological model to evaluate the prevention and treatment effects of various drugs and active ingredients on diseases. In all biological models, Caenorhabditis elegans is a kind of organism that is easy to observe and cultivate, has a clear genetic background and a high degree of signal pathway conservation with humans. A variety of active ingredients such as polyphenols, polysaccharides, polypeptides, and vitamins exist from natural products, which has extremely high physiological functions and medicinal values. It is highly reliable to evaluate the trophic and functional properties of the active ingredients from natural products in C. elegans. The functional properties of the active ingredients from natural products such as antiaging, anti-oxidation, regulating lipid metabolism, preventing and treating Alzheimer's disease in C. elegans were clarified in this review. At the same time, its possible pathways were summarized. The literature revealed that it had great effect to evaluate the nutrition and functionality of the active ingredients from natural products in C. elegans. Finally, the shortcomings in this field and suggestions for further improvement in this field have been emphasized.

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trans-Trismethoxy resveratrol decreased fat accumulation dependent on fat-6 and fat-7 in Caenorhabditis elegans

Abstract: trans-Trismethoxy resveratrol reduced fat accumulation via the regulation of FAT-6 and FAT-7, stearoyl-CoA desaturases homologs, in Caenorhabditis elegans.

Cited by 28 publications

References 57 publications

Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Fat-lowering effects of isorhamnetin are via NHR-49-dependent pathway in Caenorhabditis elegans

Butein inhibits lipogenesis in Caenorhabditis elegans

Application of Caenorhabditis elegans in the evaluation of food nutrition: A review

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