<i>Trichoplusia ni</i>(Lepidoptera: Noctuidae) Survival, Immune Response, and Gut Bacteria Changes after Exposure to<i>Azadirachta indica</i>(Sapindales: Meliaceae) Volatiles

Núñez-Mejía, G.; Valadéz-Lira, J. A.; Gómez-Flores, Ricardo; Rodríguez‐Padilla, Cristina; Tamez‐Guerra, Patricia

doi:10.1653/024.099.0104

Cited by 7 publications

(7 citation statements)

References 44 publications

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“…Furthermore, our study found that P. absoluta 2nd instar larvae treated with the botanicals (neem and garlic) had lower microbial abundance than most of the B. thuringiensis isolates . This finding agrees with previous studies, which showed that higher susceptibility of neem was associated with lower enterobacterial load in T. ni 40 and higher susceptibility to B. thuringiensis was associated with higher enterobacterial loads in Spodoptera exigua Hübner, 1808 (Lepidoptera: Noctuidae) , probably due to the mode of action of B. thuringiensis , which involves the cry proteins 41 , 42 .…”

Section: Discussionsupporting

confidence: 93%

“…Albeit minimal, there was a reduction in the abundance of these bacteria and a shift in the microbial diversity upon treatment with the B. thuringiensis isolates and the botanicals suggesting gut microbial repression by the treatments. The reduction in key gut bacteria has also been observed in Trichoplusia ni Hübner, 1800 (Lepidoptera: Noctuidae), where treatment with neem volatiles reduced Enterobacteria transcript amplification 40 . Furthermore, our study found that P. absoluta 2nd instar larvae treated with the botanicals (neem and garlic) had lower microbial abundance than most of the B. thuringiensis isolates .…”

Section: Discussionmentioning

confidence: 79%

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Interactions between Bacillus thuringiensis and selected plant extracts for sustainable management of Phthorimaea absoluta

Ochieng,

Akutse,

Ajene

et al. 2024

Sci Rep

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Phthorimaea absoluta is a global constraint to tomato production and can cause up to 100% yield loss. Farmers heavily rely on synthetic pesticides to manage this pest. However, these pesticides are detrimental to human, animal, and environmental health. Therefore, exploring eco-friendly, sustainable Integrated Pest Management approaches, including biopesticides as potential alternatives, is of paramount importance. In this context, the present study (i) evaluated the efficacy of 10 Bacillus thuringiensis isolates, neem, garlic, and fenugreek; (ii) assessed the interactions between the most potent plant extracts and B. thuringiensis isolates, and (iii) evaluated the gut microbial diversity due to the treatments for the development of novel formulations against P. absoluta. Neem recorded the highest mortality of 93.79 ± 3.12% with an LT50 value of 1.21 ± 0.24 days, Bt HD263 induced 91.3 ± 3.68% mortality with LT50 of 2.63 ± 0.11 days, compared to both Bt 43 and fenugreek that caused < 50% mortality. Larval mortality was further enhanced to 99 ± 1.04% when Bt HD263 and neem were combined. Furthermore, the microbiome analyses showed that Klebsiella, Escherichia and Enterobacter had the highest abundance in all treatments with Klebsiella being the most abundant. In addition, a shift in the abundance of the bacterial genera due to the treatments was observed. Our findings showed that neem, garlic, and Bt HD263 could effectively control P. absoluta and be integrated into IPM programs after validation by field efficacy trials.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 79%

Interactions between Bacillus thuringiensis and selected plant extracts for sustainable management of Phthorimaea absoluta

Ochieng,

Akutse,

Ajene

et al. 2024

Sci Rep

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“…Only one report described assays against the cabbage looper T. ni , where volatile organic compounds from A. indica stems promoted significant neonatal and larval mortality (24 and 77%, respectively) at 1 g doses and an LD 50 of 5.6 g after 7 days ( Table 17 ) [ 71 ].…”

Section: Insecticidal Compounds and Plant Extractsmentioning

confidence: 99%

Insecticidal and Nematicidal Contributions of Mexican Flora in the Search for Safer Biopesticides

Hernández-Carlos

Gamboa‐Angulo

2019

Molecules

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Plant metabolites have been used for many years to control pests in animals and to protect crops. Here, we reviewed the available literature, looking for the species of Mexican flora for which extracts and metabolites have shown activity against pest insects and parasitic nematodes of agricultural importance, as well as against nematodes that parasitize domestic cattle. From 1996 to 2018, the search for novel and eco-friendly biopesticides has resulted in the identification of 114 species belonging to 36 botanical families of Mexican plants with reported biological effects on 20 insect species and seven nematode species. Most plant species with detected pesticide properties belong to the families Asteraceae, Fabaceae, and Lamiaceae. Eighty-six metabolites have been identified as pesticidal active principles, and most have been terpenoids. Therefore, the continuation and intensification of this area of research is very important to contribute to the generation of new products that will provide alternatives to conventional pesticide agents. In addition, future studies will contribute to the recognition and dissemination of the importance of propagating plant species for their conservation and sustainable use.

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“…P. interpunctella larvae were reared on a cracked wheat diet (McGaughey and Beeman, 1988) at 25ºC ± 2ºC, 55 ± 10% relative humidity, and 16h light/8h dark photoperiod. For gut bacterial assays, larvae were reared under normal artificial diet (Nuñez-Mejía et al, 2016) (Fig. S1), artificial diet (wheat agar, wheat germ, soy flour and sugar sterilized by autoclave, and vitamin solution sterilized by membrane filtration and added after artificial diet temperature was lower than 60°C), and/or antibiotic treated agar-based artificial diet, where antibiotic solution was sterilized by membrane filtration and added after artificial diet temperature was lower than 60°C.…”

Section: Insect Colonymentioning

confidence: 99%

Regulation by gut bacteria of immune response, Bacillus thuringiensis susceptibility and hemolin expression in Plodia interpunctella

Orozco-Flores

Valadéz-Lira

Oppert

et al. 2017

Journal of Insect Physiology

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Plodia interpunctella (Hübner) is an important stored grain insect pest worldwide, and the first lepidopteran with reported resistance to Bacillus thuringiensis (Bt) toxins. Since gut bacteria may affect Bt insecticidal activity, we evaluated whether P. interpunctella lacking gut bacteria had differences in immune responses and susceptibility to the Bt formulation, Bactospeine. In order to clear gut bacteria, third instar larvae were reared on artificial diet containing antibiotics, or were obtained from sterilized eggs and reared under sterile conditions, and larvae were fed diets with or without Bt. Mortality was significantly lower (p<0.05) in bacteria-free larvae treated with Bt, compared with Bt-treated larvae with unaffected gut bacteria. The number of hemocytes was lower in control and Bt-treated larvae, but was significantly higher (p<0.001) in larvae treated with antibiotics and Bt, and larvae from presterilized eggs and reared on sterile diet had the highest number of hemocytes. Phenoloxidase activity was significantly lower (p<0.05) in Bt-treated larvae from presterilized eggs reared on antibiotics for 24h or in larvae reared on antibiotic-treated diets prior to Bt introduction compared with those fed control diet. Hemolin gene expression was reduced in larvae fed Bt diets compared with control and was not detected in larvae treated with antibiotics. Larvae from sterilized eggs and fed sterile diet never reached the pupal stage. Therefore, the loss of gut bacteria in P. interpunctella larvae affected the host immune response and expression of the hemolin gene, and significantly reduced susceptibility to Bt.

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Trichoplusia ni(Lepidoptera: Noctuidae) Survival, Immune Response, and Gut Bacteria Changes after Exposure toAzadirachta indica(Sapindales: Meliaceae) Volatiles

Abstract: BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 7 publications

References 44 publications

Interactions between Bacillus thuringiensis and selected plant extracts for sustainable management of Phthorimaea absoluta

Interactions between Bacillus thuringiensis and selected plant extracts for sustainable management of Phthorimaea absoluta

Insecticidal and Nematicidal Contributions of Mexican Flora in the Search for Safer Biopesticides

Regulation by gut bacteria of immune response, Bacillus thuringiensis susceptibility and hemolin expression in Plodia interpunctella

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