Brazil has the greatest biodiversity of non-human primates (NHP) in the world. 1 One strategy implemented for their conservation in Brazil is the ex situ maintenance and reproduction of the most critically endangered species. 2 In captivity, primates are generally allocated in groups and in environments smaller than their natural habitat, have more contact with humans, and are exposed to different management conditions. These circumstances can determine stress and a drop in primates' immunity, favoring parasitic infections. 3,4 Among the parasites, the Phylum Acanthocephala stands out. These can be transmitted to NHP through the ingestion of infected arthropods, mainly cockroaches and beetles, that can determine morbidity and mortality. 5 Therefore, this study aimed to report two cases of infection by Prosthernorchis elegans in captive NHP in Rio de Janeiro, Brazil.