<i>Turnip mosaic virus</i>and the quest for durable resistance

Walsh, John A.; Jenner, Carol E.

doi:10.1046/j.1364-3703.2002.00132.x

Cited by 188 publications

(124 citation statements)

References 97 publications

(108 reference statements)

Supporting

Mentioning

116

Contrasting

Unclassified

Order By: Relevance

“…1), is a Ϸ42-kDa nonstructural protein and is one of the least well characterized Potyvirus proteins. It may be involved in virus replication, pathogenicity, resistance breaking, and systemic infection (6,9,(21)(22)(23). It is interesting that previous investigations into the function and localization of P3 have produced conflicting results (6).…”

Section: Discussionmentioning

confidence: 81%

An overlapping essential gene in the Potyviridae

Chung

Miller

Atkins

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

768

502

View full text Add to dashboard Cite

The family Potyviridae includes >30% of known plant virus species, many of which are of great agricultural significance. These viruses have a positive sense RNA genome that is Ϸ10 kb long and contains a single long ORF. The ORF is translated into a large polyprotein, which is cleaved into Ϸ10 mature proteins. We report the discovery of a short ORF embedded within the P3 cistron of the polyprotein but translated in the ؉2 reading-frame. The ORF, termed pipo, is conserved and has a strong bioinformatic coding signature throughout the large and diverse Potyviridae family. Mutations that knock out expression of the PIPO protein in Turnip mosaic potyvirus but leave the polyprotein amino acid sequence unaltered are lethal to the virus. Immunoblotting with antisera raised against two nonoverlapping 14-aa antigens, derived from the PIPO amino acid sequence, reveals the expression of an Ϸ25-kDa PIPO fusion product in planta. This is consistent with expression of PIPO as a P3-PIPO fusion product via ribosomal frameshifting or transcriptional slippage at a highly conserved G 1-2A6-7 motif at the 5 end of pipo. This discovery suggests that other short overlapping genes may remain hidden even in well studied virus genomes (as well as cellular organisms) and demonstrates the utility of the software package MLOGD as a tool for identifying such genes.P3 ͉ PIPO ͉ Potyvirus ͉ Turnip mosaic virus ͉ frameshift

show abstract

Section: Discussionmentioning

confidence: 81%

An overlapping essential gene in the Potyviridae

Chung

Miller

Atkins

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

768

502

View full text Add to dashboard Cite

show abstract

“…TuMV infects a wide range of plant species, mostly from the family Brassicaceae (Walsh & Jenner, 2002). TuMV, like other potyviruses, is transmitted by aphids in a nonpersistent manner.…”

Section: Introductionmentioning

confidence: 99%

Phylodynamic evidence of the migration of turnip mosaic potyvirus from Europe to Australia and New Zealand

Yasaka

Ohba

Schwinghamer

et al. 2015

Journal of General Virology

View full text Add to dashboard Cite

Turnip mosaic virus (TuMV) is a potyvirus that is transmitted by aphids and infects a wide range of plant species. We investigated the evolution of this pathogen by collecting 32 isolates of TuMV, mostly from Brassicaceae plants, in Australia and New Zealand. We performed a variety of sequence-based phylogenetic and population genetic analyses of the complete genomic sequences and of three non-recombinogenic regions of those sequences. The substitution rates, divergence times and phylogeographical patterns of the virus populations were estimated. Six inter-and seven intralineage recombination-type patterns were found in the genomes of the Australian and New Zealand isolates, and all were novel. Only one recombination-type pattern has been found in both countries. The Australian and New Zealand populations were genetically different, and were different from the European and Asian populations. Our Bayesian coalescent analyses, based on a combination of novel and published sequence data from three nonrecombinogenic protein-encoding regions, showed that TuMV probably started to migrate from Europe to Australia and New Zealand more than 80 years ago, and that distinct populations arose as a result of evolutionary drivers such as recombination. The basal-B2 subpopulation in Australia and New Zealand seems to be older than those of the world-B2 and -B3 populations. To our knowledge, our study presents the first population genetic analysis of TuMV in Australia and New Zealand. We have shown that the time of migration of TuMV correlates well with the establishment of agriculture and migration of Europeans to these countries.

show abstract

“…Last, only CMV is efficiently seed transmitted in different species of the Brassicaceae, including A. thaliana (Pagán & García-Arenal 2010, unpublished results). The molecular genetics of these viruses has been extensively analysed, and the population genetics of CMV and TuMV has also been studied in detail (for reviews, see Hollings & Stone 1972;Carrington et al 1987Carrington et al , 1989Haas et al 2002;Walsh & Jenner 2002;Palukaitis & García-Arenal 2003).…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis thaliana as a model for the study of plant–virus co-evolution

Pagán

Fraile

Fernández‐Fueyo

et al. 2010

Phil. Trans. R. Soc. B

104

106

View full text Add to dashboard Cite

Understanding plant -virus coevolution requires wild systems in which there is no human manipulation of either host or virus. To develop such a system, we analysed virus infection in six wild populations of Arabidopsis thaliana in Central Spain. The incidence of five virus species with different life-styles was monitored during four years, and this was analysed in relation to the demography of the host populations. Total virus incidence reached 70 per cent, which suggests a role of virus infection in the population structure and dynamics of the host, under the assumption of a host fitness cost caused by the infection. Maximum incidence occurred at early growth stages, and co-infection with different viruses was frequent, two factors often resulting in increased virulence. Experimental infections under controlled conditions with two isolates of the most prevalent viruses, cauliflower mosaic virus and cucumber mosaic virus, showed that there is genetic variation for virus accumulation, although this depended on the interaction between host and virus genotypes.Comparison of Q ST -based genetic differentiations between both host populations with F ST genetic differentiation based on putatively neutral markers suggests different selection dynamics for resistance against different virus species or genotypes. Together, these results are compatible with a hypothesis of plant -virus coevolution.

show abstract

Turnip mosaic virusand the quest for durable resistance

Abstract: Aphid transmitted in the non-persistent manner, by at least 89 species, including Myzus persicae and Brevicoryne brassicae. Useful website: http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/57010072.htm.

Cited by 188 publications

References 97 publications

An overlapping essential gene in the Potyviridae

An overlapping essential gene in the Potyviridae

Phylodynamic evidence of the migration of turnip mosaic potyvirus from Europe to Australia and New Zealand

Arabidopsis thaliana as a model for the study of plant–virus co-evolution

Contact Info

Product

Resources

About