Unifoliata‐Afila interactions in pea leaf morphogenesis

Abstract

Auxin concentration and response differences characterize the antagonistic relationship between AF and UNI in pea leaf development. The mechanism involves modulation of auxin mediated by one or both genes; UNI is expressed in and promotes high auxin levels, and AF suppresses auxin levels.

Leaf development and morphogenesis

“…Correspondingly, uni mutant leaves, as well as leaves of the M. truncatula uni ortholog SINGLE LEAFLET (SGL) mutant, have reduced complexity (Gourlay et al, 2000;Wang et al, 2008). In addition, prolonged UNI expression leads to increased blastozone activity in the complex leaves of afila (af ), cochleata (coch) and afila tendril-less (af tl) mutant plants (Champagne et al, 2007;DeMason et al, 2013;DeMason and Chetty, 2011;Gourlay et al, 2000;Hofer et al, 2001).…”

Phenotypic characterization of the CRISPA (ARP gene) mutant of pea (Pisum sativum; Fabaceae): A reevaluation

“…For phenotypic characterization, seeds were sown in late August. For gene expression analysis, plants were grown in an EGC model GC15 growth chamber (Chagrin, Ohio, USA), with a combination of incandescent, halogen, and sodium light bulbs, with 8 h light/16 h dark, and 20°C day and 15°C night regime as described previously (DeMason and Chetty, 2011; DeMason et al, 2013). Photosynthetically active radiation (PAR) was approximately 340 µmol·m −2 ·s −2 at plant level.…”

“…Plants were grown in the growth chamber until leaves at nodes 10–12 were exposed (approximately 1 mo) as described previously (DeMason and Chetty, 2011; DeMason et al, 2013). Shoot tips, leaf primordia, and individual leaflets were stained and prepared as described previously (DeMason and Polowick, 2009).…”

“…Samples were observed on a Nikon SMZ‐10 dissecting microscope with reflective light from a fiber optic light source and photographs were taken with an Infinity 1 digital camera. Quantitative assays of shoot tips were done as previously described (DeMason and Polowick, 2009; DeMason et al, 2013.) A Student t test was performed to compare the means using GraphPad Prism, and significance was defined at the 0.05% level.…”

“…Six genotypes from the set of near‐isogenic lines ( WT , af , tl , af tl , cri , and uni‐tac ) were used for analysis. Plants were grown in the growth chamber and shoot tips were collected when leaves at nodes 10–12 were exposed as described previously (DeMason et al, 2013). Total RNA was isolated, then first strand cDNA synthesis and SYBR green assays were carried out as described previously (DeMason and Chetty, 2011).…”