Ureaplasma urealyticumModulates Endotoxin-Induced Cytokine Release by Human Monocytes Derived from Preterm and Term Newborns and Adults

Abstract

We previously observed that Ureaplasma urealyticum respiratory tract colonization in infants with a birth weight of <1,250 g was associated with increases in the tracheal aspirate proinflammatory cytokines tumor necrosis factor alpha (TNF-␣) and interleukin-8 (

Intra-uterine experimental infection by Ureaplasma diversum induces TNF-α mediated womb inflammation in mice

“…In vitro studies demonstrated that U. diversum was able to induce murine macrophages to produce TNF-α in a dose dependent way (ChelmonskaSoyta et al 1994). Furthermore, U. urealyticum stimulates the release of TNF-α by macrophages and monocytes from human umbilical cord blood (Manimtim et al 2001). Infections with U. diversum in the reproductive tract may, at fi rst, not stimulate recruitment of cells to the site.…”

Lung Diseases - Selected State of the Art Reviews

“…Additionally, it has been recently demonstrated that M. pneumoniae infection induces reactive oxygen species and DNA damage in human lung cells [Sun et al, 2008]. A number of studies show that Ureaplasma urealyticum induce the production of TNF-α, IL-8, IL-6 [Li et al, 2000a;Manimtim et al, 2001;Peltier et al, 2008], NF-B and nitric oxide [Li et al, 2000b] by human macrophages and monocytes. Infection assays in macrophages performed in combination with LPS showed that U. urealyticum enhances the proinflammatory response to a second infection by blocking expression of counterregulatory cytokines (IL-6 and IL-10), predisposing the preterm infant to prolonged and dysregulated inflammation, lung injury, and impaired clearance of secondary infections [Manimtim et al, 2001].…”

“…Among premature infants, respiratory tract colonization with genital ureaplasmas has been associated with pneumonia, chronic lung disease, infant wheezing, respiratory distress syndrome, acute respiratory insufficiency, and increased mortality [Cultrera et al, 2006;Kafetzis et al, 2004]. For some infants, ureaplasmal infection triggers a vigorous response in the lungs involving the elevation of adhesion molecules, collagenases, proinflammatory cytokines and neutrophil activation and migration, which increase the risk of developing bronchopulmonary dysplasia characterized by delayed alveolarization, chronic inflammation, and fibrosis [Manimtim et al, 2001;Schelonka & Waites, 2007]. Additionally, free radical generation and oxidative injury induced by recruited neutrophils could contribute to lung damage [Buss et al, 2003].…”

“…Data from Dakhama et al, 2003;Kazachkov et al, 2002;Li et al, 2000a;Manimtim et al, 2001;Peltier et al, 2008 ;Stancombe et al, 1993;Yang et al, 2004. Table 2.…”

“…A number of studies show that Ureaplasma urealyticum induce the production of TNF-α, IL-8, IL-6 [Li et al, 2000a;Manimtim et al, 2001;Peltier et al, 2008], NF-B and nitric oxide [Li et al, 2000b] by human macrophages and monocytes. Infection assays in macrophages performed in combination with LPS showed that U. urealyticum enhances the proinflammatory response to a second infection by blocking expression of counterregulatory cytokines (IL-6 and IL-10), predisposing the preterm infant to prolonged and dysregulated inflammation, lung injury, and impaired clearance of secondary infections [Manimtim et al, 2001]. Furthermore, U. urealyticum stimulates macrophages to produce vascular endothelial grow factor (VEGF) and intercellular adhesion molecule-1 (ICAM-1) in vitro, which are potentially associated with both early and later pathological changes in the lung during the development of chronic lung disease [Li et al, 2002a].…”