The debate on agencification in the public sector has traditionally overlooked the influence of space on how autonomy unfolds in practice. Starting with the contention that organizing and space are interweaved, the study analyzes the early stages of the agencification process of three Italian museums and reveals that space is a constraint to, and an enabler of, de facto autonomy for newly created entities. Space is a constraint when spatial changes are temporally misaligned with the agencification process, including directorship cycles. On the other hand, space enables de facto autonomy, as it is purposely modified to accommodate new demands and needs by newly appointed managers. The paper maintains that policy makers and site managers should seriously consider space when dealing with agencification reforms, paying attention to the revenue and cost implications of spatial solutions, and the areas of responsibility concerning buildings and objects.