Vernonia condensataBaker (Asteraceae): A Promising Source of Antioxidants

Abstract

The present study evaluated the antioxidant potential of Vernonia condensata Baker (Asteraceae). Dried and powdered leaves were exhaustively extracted with ethanol by static maceration followed by partition to obtain the hexane, dichloromethane, ethyl acetate, and butanol fractions. Total phenols and flavonoids contents were determined through spectrophotometry and flavonoids were identified by HPLC-DAD system. The antioxidant activity was assessed by DPPH radical scavenging activity, TLC-bioautography, reducing power of Fe+3, phosphomolybdenum, and TBA assays. The total phenolic content and total flavonoids ranged from 0.19 to 23.11 g/100 g and from 0.13 to 4.10 g/100 g, respectively. The flavonoids apigenin and luteolin were identified in the ethyl acetate fraction. The IC50 of DPPH assay varied from 4.28 to 75.10 µg/mL and TLC-bioautography detected the antioxidant compounds. The reducing power of Fe+3 was 19.98 to 336.48 μg/mL, while the reaction with phosphomolybdenum ranged from 13.54% to 32.63% and 56.02% to 135.00% considering ascorbic acid and rutin as reference, respectively. At 30 mg/mL, the ethanolic extract and fractions revealed significant effect against lipid peroxidation. All these data sustain that V. condensata is an important and promising source of bioactive substances with antioxidant activity.

Eco-friendly synthesis, characterization, in vitro and in vivo anti-inflammatory activity of silver nanoparticle-mediated <em>Selaginella myosurus</em> aqueous extract

“…It is well documented that therapeutic effects of herbal medicine are due to active components present in their extract. 6 However, most of these active components possess insoluble character leading to lower bioavailability, increased systemic clearance, 7 and required unnecessarily high systemic administration. 8 Applying nanotechnology to herbal drugs may lead to the development of nano herbal products, which will open a new era of herbal discovery because nanosized drug delivery systems have many advantages to offer including solubility, bioavailability, pharmacological activity enhancement, protection from toxicity, sustained delivery, and protection from physical and chemical degradation.…”

Evaluation of phytochemical components from ethyl acetate fraction of Vernonia calvoana using gas chromatography-mass spectrometry analysis and its antioxidants activities

“…The genus, overtime, have been popularly used in the management of different disorders including inflammation, malaria, fever, worms, pain, diuresis, cancer, abortion, and several gastrointestinal problems (Alves and Neves 2003). Also based on pharmacological use, Vernonia species have been documented to possess hypotensive activity (Achola et al, 1996), phototoxic, antibacterial and antiinflammatory (Perez-Amador et al, 2008), immunomodulatory (Nergard et al, 2004), antioxidants (da Silva et al, 2013) and anti-histaminic activity (Laekeman et al, 1983). These activities reported may be attributed to diverse secondary metabolites such as flavonoids, anthraquinones, polyphenols, tannins, alkaloids, sesquiterpene lactones, phenol, steroidal saponins, cardiac glycosides, carotenoids and high nutritive components present in this species (Ejoh et al, 2005;Igile et al, 2013).…”

“…The molecule have the ability of neutralizing free radical by donating electrons and further prevent damages that may be caused by this radical to cell and tissue (da Silva et al, 2013). Fruits, vegetables and herbs are good sources of these compounds which are known as phytochemicals including flavonoids, ascorbic acids etc.…”

Pharmacological potential of Palicourea rigida kunth: A possible participation of flavonoid compounds

“…The methodology used in this analysis was described by Silva et al (2013) previously validated by the Laboratory of Natural Products/Institute of Biological Sciences/UFJF. The HPLC system consisted of an Agilent Technologies 1200 Series with a PDA detector and an automatic injector.…”