Vibrio anguillarum serovars associated with vibriosis in fish

Abstract

A total ol" 317 Vibrio anguillantm strains isolated from diseased fish together with 14 V. anguillarum serogroup O2 and V. ordalii type strains were serotyped using the European scrotyping system. Marked speeies difterenees were reeorded. In isolates from belonged to other serogroups or were non-typeablc. Figures for turbot were similar to those from salmonids. In 32 isolates from sea bass, sea bream and mullet, most strains belonged to serogroup Ol. while one was O2a. one O7, and the rest non-typcable In cod sero\ar O2 was dominant while only a minor proportion belonged to other serogroups or were non-typeable. The eel isolates belonged equally to serovars O2 and O3. All O2 strains were subtyped with absorbed O2a and O2b antisera. O2a was dominant in all fish speeies. but in eod. the relative number of O2b isolates was eonsiderably higher than in other fish speeies.

Plasmid profiles, restriction fragment length polymorphisms and O-serotypes amongVibrio anguillarumisolates

“…Strains were isolated in our laboratory or received from various other laboratories. 0-serotyping 0-serotyping was carried out using the slide agglutination method as previously described [23,24]. The strains were tested for reactivity with the 10 antisera, 01-010, described in the European serotyping system [24], and with seven additional antisera, temporarily designated [26] and subjected to electrophoresis in 0 8 % agarose gels (SeaKem GTG, FMC BioProducts, Rockland, ME, USA) in TAE (Tris 40 mm, sodium acetate 5 mM, ethylenediaminetetraacetate (EDTA) 1 mM; pH 8 0).…”

Restriction fragment length polymorphism of the pMJ101-like plasmid and ribotyping in the fish pathogenVibrio ordalii

“…All strains were stored at -80°C until used and propagated on blood agar plates (Marine agar (Difco) supplemented with 5 % sterile, citratestabilized calf blood), incubated at 20°C for 2 days. 0-serotyping 0-serotyping was carried out using the slide agglutination method as previously described [9,19]. Absorbed 02a and 02b sera were obtained as described by Larsen and colleagues [9].…”

“…0-serotyping 0-serotyping was carried out using the slide agglutination method as previously described [9,19]. Absorbed 02a and 02b sera were obtained as described by Larsen and colleagues [9].…”

“…It was recently demonstrated that V. ordalii and V. anguillarum were easily distinguished by ribotyping [8]. V. ordalii cross-reacts serologically with V. anguillarum serogroup 02a [9] although minor antigenic differences have been demonstrated. Thus, Mutharia and Amor [10] were able to generate monoclonal antibodies that recognized distinct species-specific epitopes in 0-antigens of V. anguillarum and V. ordalii and the results of Mutharia and colleagues [11] demonstrated both common antigens and species-specific antigens using absorbed and unabsorbed polyclonal antisera, generated in fish and rabbits.…”

Survival behaviour and virulence of the fish pathogen Vibrio ordalii in seawater microcosms

“…Numerous studies describe V. ordalii isolated from salmon as a biochemically, serologically and geneti-cally homogeneous taxon (Mutharia et al 1993, Larsen et al 1994, Grisez & Ollevier 1995, Tiainen et al 1995, Toranzo et al 1997, although Silva-Rubio et al (2008) suggested the possibility that the Chilean strains constitute a new serological subgroup of this bacterial species. In addition to the fact that its taxonomic classification is still evolving, our understanding of the pathogenic mechanisms of V. ordalii is likewise incomplete.…”