<i>Vibrio cholerae</i>persistence in aquatic environments and colonization of intestinal cells: involvement of a common adhesion mechanism

Zampini, Massimiliano; Pruzzo, Carla; Bondre, Vijay P.; Tarsi, R.; Cosmo, Mariangela; Bacciaglia, Alessandro; Chhabra, Arvind; Srivastava, Reeta; Srivastava, Bhavana

doi:10.1016/j.femsle.2005.01.052

Cited by 50 publications

(53 citation statements)

References 39 publications

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“…V. cholerae is known to adhere to different surfaces, such as the human lumen (20), chironomid egg masses (6), chitin (46), zooplankton (9,18), and cornstarch granules (15). Its ability to adhere is related to the survival of the bacteria in the environment (9), as it facilitates both adherence to available nutrition sources and protection against environmental stresses (52). It is also associated with colonization in the human intestine, which is a prerequisite for establishment of a productive infection (20,41).…”

Section: Discussionmentioning

confidence: 99%

Mechanisms Involved in Governing Adherence of Vibrio cholerae to Granular Starch

Niderman-Meyer

Zeidman

Shimoni

et al. 2010

Appl Environ Microbiol

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Vibrio cholerae has been shown to adhere to cornstarch granules. The present work explored the mechanisms involved in this adhesion and the possibility of its occurrence in vivo. The findings suggest that both specific and nonspecific interactions are involved in the adhesion. Nonspecific hydrophobic interactions may play a role, since both V. cholerae and cornstarch granules exhibited hydrophobic properties when they were tested using a xylene-water system. In addition, the presence of bile acids reduced the adhesion. The adhesion also involves some specific carbohydrate-binding moieties on the cell surface, as reflected by reduced adhesion following pretreatment of the bacteria with proteinase K and sodium m-periodate. Further investigations supported these observations and showed that media containing low-molecular-weight carbohydrates had a significant inhibitory effect. Binding cell lysate to starch granules and removing the adhered proteins using either glucose or bile acids led to identification (by liquid chromatography-tandem mass spectrometry analysis) of several candidate V. cholerae outer membrane-associated starch-binding proteins. Different sets of proteins were isolated by removal in a glucose solution or bile acids. When the upper gastrointestinal tract conditions were simulated in vitro, both bile salts and the amylolytic activity of the pancreatic juices were found to have an inhibitory effect on the adherence of V. cholerae to starch. However, during acute diarrhea, this inhibitory effect may be significantly reduced due to dilution, suggesting that adhesion does occur in vivo. Such adhesion may contribute to the beneficial effects observed following administration of granular starch-based oral rehydration solutions to cholera patients.

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Section: Discussionmentioning

confidence: 99%

Mechanisms Involved in Governing Adherence of Vibrio cholerae to Granular Starch

Niderman-Meyer

Zeidman

Shimoni

et al. 2010

Appl Environ Microbiol

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“…Two of our environmental strains (EVC4 and EVC5) with tcpA 2 /toxT 2 genotype showed strong adhesive properties to the cells. Non-fimbrial adhesive factors such as MSHA (mannose sensitive haemagglutinin antigen) and 53 kDa CBP (chitin binding protein) have been suggested as colonization factor candidates (Kirn et al, 2005;Zampini et al, 2005;Vezzulli et al, 2008). Other colonization factors such as TcpF, TcpI and AcfB have recently been identified (Kirn et al, 2003;Chaparro et al, 2010).…”

Section: Autoagglutination and Adherence Assaymentioning

confidence: 99%

Characterization of pathogenicity island prophage in clinical and environmental strains of Vibrio cholerae

Mohammadi-Barzelighi

Bakhshi

Lari

et al. 2011

Journal of Medical Microbiology

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In this study 86 isolates of Vibrio cholerae were analysed for their adhesive properties and the presence of pathogenicity island genes. With the exception of three isolates, all of the other clinical isolates (92.5 %) contained an intact TCP (toxin-co-regulated pilus) gene cluster. In contrast, 95 % of all environmental non-O1-non-O139 isolates were negative for the TCP gene cluster. The majority of clinical isolates (82.5 %) possessed the complete vibrio pathogenicity island (VPI) gene cluster and had a similar RFLP pattern, while only a single environmental strain possessed an almost complete VPI cluster (lacking 0.4 kb in the tcpA and toxT region). The result showed that the isolates with tcpA + /toxT + had a strong attachment for HT-29 and Vero cells, whereas isolates with tcpA + /toxT " or tcpA " /toxT " genomic characteristics showed no autoagglutination and weak attachment for the cell lines. Two environmental strains (tcpA " /toxT " ) showed strong adhesive properties to the cell lines, indicating that non-fimbrial adhesive factors are involved in the environmental V. cholerae strains in the absence of TCP.

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“…Compared to freeliving organisms, organisms associated with zooplankton have been shown to be different in morphology, size and metabolic activity (Tarsi et al 2000 cagA plankton provides stable microhabitats for microorganisms in which cells are more likely to survive stressful conditions found in seawater than in free form (Huq et al 1983, Colwell & Huq 1994, WinieckaKrusnell et al 2002. This assumption is supported by numerous studies on pathogenic bacteria other than Helicobacter pylori, in which the ecological relationship between epibiotic microorganisms and copepods results in protection from stress conditions (Signoretto et al 2004, Zampini et al 2005. Zooplankton can therefore be a reservoir for pathogenic bacteria such as Vibrio spp., including V. cholerae (Colwell & Huq 1994).…”

Section: Discussionmentioning

confidence: 92%

“…Vibrio spp. mineralize chitin-containing substrates (Pruzzo et al 1996, Tarsi et al 2000, and thus have been found to be associated with chitincontaining copepods such as Tigriopus fulvus (Pruzzo et al 2003, Zampini et al 2005. The identification of the environmental strain obtained by using the species-specific target gene vacA and the confirmatory DNA sequencing indicated that the isolate was a true Helicobacter pylori strain.…”

Section: Discussionmentioning

confidence: 99%

Detection of Helicobacter pylori associated with zooplankton

Cellini

Campli²,

Grande³

et al. 2005

Aquat. Microb. Ecol.

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Helicobacter pylori was isolated from marine zooplankton, and characterized by standard microbiological tests, by PCR amplification of vacA and cagA gene fragments, and by comparative sequence analysis of the vacA PCR product. In a viable-but-non-culturable (VBNC) state, this isolate, as well as the reference strain H. pylori ATCC 43629, could be re-activated only when incubated in the presence of the marine copepod Tigriopus fulvus, and not in its absence. Isolate and type strain of H. pylori were found to be associated to the surface of T. fulvus, which supports speculations about a potential role of copepods in H. pylori survival and transmission.KEY WORDS: Helicobacter pylori · Zooplanktonic organisms · Transmission · Seawater · Viable-butnon-culturable (VBNC) state · Tigriopus fulvus Resale or republication not permitted without written consent of the publisherAquat Microb Ecol 40: [115][116][117][118][119][120] 2005 pylori cells from seawater samples and associated to zooplankton. An isolate and the type strain of H. pylori were subsequently used to study potential interactions with zooplankton. For this purpose, the marine copepod Tigriopus fulvus (Fisher 1860) found in rockpools of the Ligurian coast (Carli et al. 1993) was used as a model organism. MATERIALS AND METHODSWater sampling and processing. Water samples of 10 l were collected with a Niskin bottle at a depth of 5 m from the Adriatic Sea (42°29' 41'' N, 14°12' 19'' E), about 500 m from the coast of Pescara, Italy. Water samples were refrigerated at 4°C and processed within 4 h. They were first passed through a 200 µm mesh nylon filter (Idromar). Filters with retained organisms were transferred to sterile plastic containers, and covered with 10 ml of sterile seawater and sterile glassbeads. After detachment of organisms from the filter by a minishaker (IKA WORKS) at 600 rpm for 1 min, the samples were twice centrifuged with sterile seawater at 200 × g for 10 min to remove free bacteria, and the remaining organisms finally resuspended in 10 ml of sterile seawater.The filtered water was subsequently passed through a 64 µm mesh nylon filter, which was subsequently treated as described above. The remaining filtered water was finally passed through 0.22 µm pore-size standard membrane filters (Pall Gelman Laboratory) to detect free cells of Helicobacter pylori and the filter was treated as above. From each sample, 3 equal aliquots were prepared before culture processing.Isolation and characterization of Helicobacter pylori. From samples obtained on these different filters, 100 µl were directly spread on non-selective medium with 10% of defibrinate horse blood (Chocolate agar, CA, OXOID) supplemented with 1% of IsoVitaleX (Becton Dickinson), and on Campylobacter selective medium (CP, OXOID) and incubated at 37°C under microaerophilic conditions (Campy Pak Jar, OXOID) for 5 to 7 d. Plates were checked every day and potential colonies of Helicobacter pylori were subcultured on CA and CP under microaerophilic conditions. The remaining sea wat...

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Vibrio choleraepersistence in aquatic environments and colonization of intestinal cells: involvement of a common adhesion mechanism

Cited by 50 publications

References 39 publications

Mechanisms Involved in Governing Adherence of Vibrio cholerae to Granular Starch

Mechanisms Involved in Governing Adherence of Vibrio cholerae to Granular Starch

Characterization of pathogenicity island prophage in clinical and environmental strains of Vibrio cholerae

Detection of Helicobacter pylori associated with zooplankton

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