<i>Vibrio vulnificus</i> infection and metalloprotease

Miyoshi, Shin Ichi

doi:10.1111/j.1346-8138.2006.00139.x

Cited by 52 publications

(31 citation statements)

References 38 publications

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“…The lower ODV content of deletion mutant OBs could be a result of the adhesion capacity of this protein (18,39), which may aggregate virions during the process of OB formation. SF29 contains a catalytic domain that includes a zinc-binding motif (HEXXH), suggesting that this protein could also have protease activity.…”

Section: Discussionmentioning

confidence: 99%

Sf29 Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Is a Viral Factor That Determines the Number of Virions in Occlusion Bodies

Simón

Williams

Asensio

et al. 2008

J Virol

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The genome of Spodoptera frugiperda multiple nucleopolyhedrovirus (NPV) was inserted into a bacmid (Sfbac) and used to produce a mutant lacking open reading frame 29 (Sf29null). Sf29null bacmid DNA was able to generate an infection in S. frugiperda. Approximately six times less DNA was present in occlusion bodies (OBs) produced by the Sf29null bacmid in comparison to viruses containing this gene. This reduction in DNA content was consistent with fewer virus particles being packaged within Sf29null bacmid OBs, as determined by fractionation of dissolved polyhedra and comparison of occlusion-derived virus (ODV) infectivity in cell culture. DNA from Sfbac, Sf29null, or Sf29null-repair, in which the gene deletion had been repaired, were equally infectious when used to transfect S. frugiperda. All three viruses produced similar numbers of OBs, although those from Sf29null were 10-fold less infectious than viruses with the gene. Insects infected with Sf29null bacmid died ϳ24 h later than positive controls, consistent with the reduced virus particle content of Sf29null OBs. Transcripts from Sf29 were detected in infected insects 12 h prior to those from the polyhedrin gene. Homologs to Sf29 were present in other group II NPVs, and similar sequences were present in entomopoxviruses. Analysis of the Sf29 predicted protein sequence revealed signal peptide and transmembrane domains, but the presence of 12 potential N-glycosylation sites suggest that it is not an ODV envelope protein.Other motifs, including zinc-binding and threonine-rich regions, suggest degradation and adhesion functions. We conclude that Sf29 is a viral factor that determines the number of ODVs occluded in each OB.

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Section: Discussionmentioning

confidence: 99%

Sf29 Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Is a Viral Factor That Determines the Number of Virions in Occlusion Bodies

Simón

Williams

Asensio

et al. 2008

J Virol

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“…Characterized M9As are collagenases, and the PPC domain from Vibrio vulnificus is thought to interact with type IV collagen (45). No structure of PPC-M9A has been reported.…”

Section: Figmentioning

confidence: 99%

Structural Comparison of ColH and ColG Collagen-Binding Domains from Clostridium histolyticum

et al. 2013

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“…Although human infections are rare, V. vulnificus disease causes the highest hospitalization (91.3%) and mortality (34.4%) rates of all foodborne diseases in the United States (14)(15)(16). One of the possible virulence determinants in V. vulnificus, vvp, which encodes elastolytic protease, may be a causative agent for skin lesions (16)(17)(18)(19)(20). It is well known that epidemic cholera is caused mainly by toxigenic V. cholerae O1 and O139.…”

mentioning

confidence: 99%

Season-Specific Occurrence of Potentially Pathogenic Vibrio spp. on the Southern Coast of South Korea

Lee

Jang

et al. 2017

Appl Environ Microbiol

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Vibrio species are widely distributed in warm estuarine and coastal environments, and they can infect humans through the consumption of raw and mishandled contaminated seafood. In this study, we aimed to isolate and observe the distribution of enteropathogenic Vibrio spp. from environments of the southern coast of South Korea over a season cycle. A total of 10,983 isolates of Vibrio spp. were obtained from tidal water and mud samples over a 1-year period from five sampling sites along the southwest coast of South Korea. We found that Vibrio alginolyticus (n ϭ 6,262) and Vibrio parahaemolyticus (n ϭ 1,757) were ubiquitous in both tidal water and mud year round, whereas Vibrio cholerae (n ϭ 24) and Vibrio vulnificus (n ϭ 130) were seasonally specific to summer. While all V. cholerae isolates were nontoxigenic (non-O1 and non-O139), more than 88% of V. vulnificus isolates possessed the virulence factor elastolytic protease (encoded by vvp). Interestingly, V. parahaemolyticus, which was omnipresent in all seasons, contained the virulence factors thermostable direct hemolysin (encoded by tdh) and thermostable direct hemolysin-related hemolysin (encoded by trh) in larger amounts in June (29 trh-positive strains) and September (14 tdh-, 36 trh-, and 12 tdh-and trh-positive strains) than in December (4 trh-positive strains) and February (3 tdh-positive strains), and virulence factors were absent from isolates detected in April. To understand why virulence factors were detected only in the warm season and were absent in the cold season although the locations are static, long-term monitoring and particularly seasonal study are necessary. IMPORTANCEThe presence of enteropathogenic Vibrio species (Vibrio cholerae, Vibrio parahaemolyticus, and Vibrio vulnificus), which cause acute diarrheal infection, septicemia, and wound infections upon ingestion through food and water, is usually associated with temperature. The World Health Organization (WHO) has estimated that there are 1.4 to 4.3 million cases and 28,000 to 142,000 deaths per year worldwide caused by cholera disease. In South Korea alone, consumption is as much as 52.4 kg of fish and shellfish per year per capita. Our findings suggested that seasonally specific acceleration of these possible pathogenic Vibrio spp. may threaten seafood safety and increase the risk of illness in South Korea, where local people consume raw fish during warmer months.

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Vibrio vulnificus infection and metalloprotease

Cited by 52 publications

References 38 publications

Sf29 Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Is a Viral Factor That Determines the Number of Virions in Occlusion Bodies

Sf29 Gene of Spodoptera frugiperda Multiple Nucleopolyhedrovirus Is a Viral Factor That Determines the Number of Virions in Occlusion Bodies

Structural Comparison of ColH and ColG Collagen-Binding Domains from Clostridium histolyticum

Season-Specific Occurrence of Potentially Pathogenic Vibrio spp. on the Southern Coast of South Korea

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