Vicia faba, V. sativa and Lens culinaris as new hosts for Pea necrotic yellow dwarf virus in Germany and Austria

Next Generation Sequencing for Detection and Discovery of Plant Viruses and Viroids: Comparison of Two Approaches

“…RT-qPCR was performed using published methods for PepMV (Gutiérrez-Aguirre et al, 2009 ) and for ToMV (Boben et al, 2007 ). Conventional RT-PCR was performed for PNYDV (Gaafar and Ziebell, 2016 ), STV (Sabanadzovic et al, 2009 ), ToCV (Dovas et al, 2002 ), TMV (Kumar et al, 2011 ), PLMVd (Loreti et al, 1999 ) TASVd and CLVd (Verhoeven et al, 2004 ). PCR primers designed specifically to confirm the presence of novel CCyV1 were as follows: CCyV1-fw (5′-GTCTCTCTTGCGTTGAGCCA-3′) and CCyV1-rev (5′-GGTTGCGGATAGCTCTTCCT-3′).…”

Investigating the Pea Virome in Germany—Old Friends and New Players in the Field(s)

“…Pea plants are known to be hosts to several viruses from different families, e.g., Luteoviridae, Nanoviridae, and Potyviridae. These viruses often occur in mixed infections causing a range of symptoms such as chlorosis, dwarfing, mottling, vein clearing, enations, and necrosis (Musil, 1966;Bos et al, 1988;Kraft, 2008;Gaafar et al, 2016;Gaafar and Ziebell, 2019b). Because of the recent detection of novel pea-infecting viruses, such as pea necrotic yellow dwarf virus (PNYDV), across Germany and within neighboring countries (Grigoras et al, 2010a;Gaafar et al, 2016Gaafar et al, , 2017Gaafar et al, , 2018 we were interested to know whether there are more unknown and/or undetected viruses present in this crop in Germany.…”

Molecular characterisation of the first occurrence of Pea necrotic yellow dwarf virus in Denmark

“…1 Julius Kuehn-Institute, Institute for Epidemiology and Pathogen Diagnostics,38106 Braunschweig,Germany; 2 SEGES, Danish Agriculture & Food Council F.m.b.A.,Agro Food Park15,8200 Pea necrotic yellow dwarf virus (PNYDV), a member of the Nanovirus genus, has been reported from numerous European countries causing yield loss in peas, faba beans, vetches and lentils (Grigoras et al, 2010;Grigoras et al, 2014;Gaafar et al, 2016;Gaafar et al, 2017). In July and August 2016, five faba bean (Vicia faba) samples were received from five fields in different regions of Denmark, in which 2 to 25% plants were diseased.…”

“…The samples reacted positively when analysed with ELISA using antibodies against PNYDV (Gaafar et al, 2016). DNA was extracted and PNYDV infection was confirmed by PCR using primer pairs specific for components R and S (Gaafar et al, 2017).…”