<i>Vitis vinifera</i>Must Varietal Authentication Using Microsatellite DNA Analysis (SSR)

Faria, Miguel A.; Magalhaes, R. Soares; Ferreira, Margarida A.; Meredith, Carole P.; Monteiro, FP

doi:10.1021/jf990837h

Cited by 58 publications

(56 citation statements)

References 15 publications

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“…The must DNA sample concentrations ranged from 211 to 401 ng μL −1 . The must DNA yields were higher than those reported by other authors using similar samples (Faria et al 2000(Faria et al , 2008Garcia-Beneytez et al 2002;Siret et al 2002), ranging from 10 to 20 μg mL −1 of starting material. One of the reasons that may explain the higher yields obtained may be the fact that we have used immediately frozen fresh must samples, which preserved high-quality DNA.…”

Section: Dna Extraction and Quantificationcontrasting

confidence: 70%

“…However, efficient DNA extraction and amplification from other matrices such as grape must and wine remain difficult, mainly due to: (a) plant DNA decomposition during the maceration process; (b) presence of microorganisms' DNA, namely yeasts; (c) DNA polymerase inhibitors such as polysaccharides, tannins, and polyphenols, present in matrices especially further down the processing chain. Nevertheless, several reports have been successful using must and experimental wine samples (Baleiras-Couto and Eiras-Dias 2006;Faria et al 2000;Garcia-Beneytez et al 2002;Nakamura et al 2007;Rodríguez-Plaza et al 2006;Siret et al 2000Siret et al , 2002. Grapevine collections were formerly described using ampelographic descriptions including morphological and phenological aspects (Alleweldt and Dettweiler 1986;Dettweiler 1993;Ortiz et al 2004;Santiago et al 2005).…”

Section: Introductionmentioning

confidence: 99%

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Molecular Markers for Assessing Must Varietal Origin

et al. 2012

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Wine quality and market value greatly depend on the grapevine varietal composition, which may be characteristic of specific regions. In order to defend the distinct regions, Denominations of Origin were defined to protect against fraudulent practices. In this study, we evaluated the efficiency of two microsatellite-based systems (microsatellite (SSR) and intermicrosatellite (ISSR)) for must varietal composition determination and their potential role in certification purposes. Eleven Vitis vinifera L. varieties from leaf and monovarietal must DNA samples were screened with six SSR and 14 ISSR primers to discriminate polymorphisms. Principal coordinates analysis was performed with DCENTER on the resultant data using unweighted pair group mathematical average and revealed that ISSRs markers were not suitable for certification procedures, whereas nuclear SSR markers presented a complete correspondence between leaf and must samples, demonstrating that they were adequate for traceability purposes.

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Section: Dna Extraction and Quantificationcontrasting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Molecular Markers for Assessing Must Varietal Origin

et al. 2012

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“…DNA was extracted directly from the dried leaves using the method described by Faria et al 25 based on a methodology developed by Wang et al 24 However, with this methodology, in which the final DNA pellet was re-suspended in 50 µl of Tris EDTA buffer (TE) diluted 1:10, or sterile deionized water, no DNA amplification was obtained, presumably due to the lack of purity of the extracts. In an attempt to solve the extraction problem, an efficient purification methodology was developed by our group (Faria MA, submitted for publication), which is based on the occlusion of the extract in a low melting point (LMP) agarose matrix that, after solidification, is immersed in a large volume of washing TE (10 mM Tris, 1 mM EDTA, pH = 8,0) buffer.…”

Section: Materials and Methods Plant Materials And Dna Extractionmentioning

confidence: 99%

Grapevine clones discriminated using stilbene synthase–chalcone synthase markers

Faria

Beja-Pereira

Martins³

et al. 2004

J Sci Food Agric

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Touriga Nacional is the most popular Portuguese grapevine cultivar. After twenty years of clonal selection, highly recommended qualitative and productive clones have been identified. In the genus Vitis several molecular markers have been used to characterize genetic diversity at the DNA level, namely microsatellites, ISSR (inter simple sequence repeat), AFLP (amplified fragment length polymorphism), and stilbene synthase-chalcone synthase (StSy-CHS) markers.In this work a novel pure DNA extraction methodology, which occludes the extract in an agarose matrix, thus allowing the removal of the remaining hydrophilic impurities, was used. An optimized and highly reproducible procedure for the detection of differences among Vitis vinifera L clones using the StSy-CHS gene family primer combination is described. With this methodology, 21 clones and a commercial cultivar, 'Chardonnay', were evaluated with three StSy-CHS markers. It was possible to obtain 14% discrimination within Touriga Nacional (TN) clones, identifying two different groups. The polyclonal origin of TN clones was previously verified with eight microsatellite markers. The methodology described could be used for clonal selection of grapevine cultivars.

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“…Although ion-exchange type kits provided sufficient amounts of purified DNAs from samples of canned papaya fruits, it was necessary to select suitable primer combinations to amplify the transgenes. Attempts were made to apply DNA profiling methods to identify grapevine cultivars in must and wine using SSR markers (Faria et al 2000, Siret et al 2000, Garcia-Beneytez et al 2002. Cultivar identification was partially successful with genomic DNAs recovered from grape must and wine.…”

Section: Introductionmentioning

confidence: 99%

DNA Profiling of Fresh and Processed Fruits in Pear

et al. 2006

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A DNA profiling method was developed for fresh and processed fruits in pear. Four different DNA extraction methods were examined to isolate genomic DNAs from fresh pear fruits, i.e., CTAB-based method and methods based on 3 kinds of DNA extraction kits (DNeasy Plant Mini Kit, G2 buffer & Genomic-tip20/G, Nucleon Phytopure Plant DNA Extraction Kit). All the methods enabled to recover genomic DNAs from samples of fresh fruits with such a high quality that cultivar identification could be performed based on SSR markers. Among them, the G2 buffer & Genomic-tip20/G method gave the best results for samples of fresh as well as processed fruits. Partially degraded genomic DNAs that were isolated from samples of dried fruits could be amplified by all the tested SSR markers. SSR analysis revealed that genotypes from dried fruits were identical with those of the European pear cultivar 'Bartlett', indicating that cultivar identification could be successfully performed. Severely degraded genomic DNAs less than 500 bp in size were recovered from samples of canned fruits and fruit juice. The amount and quality of the extracted DNAs were sufficient to enable amplification by primers corresponding to high copy rDNA sequences, whereas no bands were produced by primers of chloroplast DNA sequences. Out of 15 SSR loci, 9 SSRs with target sequences less than 150-160 bp could successfully amplify fragments for genomic DNAs from samples of canned fruits and fruit juice. In contrast, no amplified fragments were observed for the remaining 6 SSRs with longer target sequences. DNA profiling and cultivar identification were successfully performed by using SSR markers to amplify short target sequences less than 150 bp.

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Vitis viniferaMust Varietal Authentication Using Microsatellite DNA Analysis (SSR)

Cited by 58 publications

References 15 publications

Molecular Markers for Assessing Must Varietal Origin

Molecular Markers for Assessing Must Varietal Origin

Grapevine clones discriminated using stilbene synthase–chalcone synthase markers

DNA Profiling of Fresh and Processed Fruits in Pear

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