<i>Withania coagulans</i>Fruit Extract Reduces Oxidative Stress and Inflammation in Kidneys of Streptozotocin-Induced Diabetic Rats

Ojha, Shreesh; Alkaabi, Juma; Amir, Naheed; Azimullah, Sheikh; Agil, Ahmad; Fahim, Mohamed A.; Adem, Abdu

doi:10.1155/2014/201436

Cited by 44 publications

(28 citation statements)

References 29 publications

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“…30) Prolonged ROS leads to renal dysfunction and histologic real injury. 31) The histopathology result of this study confirmed the renal damage in diabetic mice as clear pathological changes were observed. UDCA managed to prevent this damage, confirming the renoprotective effect through ER stress blocker.…”

Section: )supporting

confidence: 78%

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Ursodeoxycholic Acid Ameliorated Diabetic Nephropathy by Attenuating Hyperglycemia-Mediated Oxidative Stress

Wang

Chen

et al. 2016

Biological & Pharmaceutical Bulletin

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Oxidative stress has a great role in diabetes and diabetes induced organ damage. Endoplasmic reticulum (ER) stress is involved in the onset of diabetic nephropathy. We hypothesize that ER stress inhibition could protect against kidney injury through anti-oxidative effects. To test whether block ER stress could attenuate oxidative stress and improve diabetic nephropathy in vivo and in vitro, the effect of ursodeoxycholic acid (UDCA), an ER stress inhibitor, on spontaneous diabetic nephropathy db/db mice, ER stress inducer or high glucose-triggered podocytes were studied. Mice were assigned to 3 groups (n 6 per group): control group (treated with vehicle), db/db group (treated with vehicle), and UDCA group (db/db mice treated with 40 mg/ kg/d UDCA). After 8 weeks treatment, mice were sacrificed. Blood and kidneys were collected for the assessment of albumin/creatinine ratio, blood urea nitrogen (BUN), serum creatinine (SCr), insulin, total cholesterol, triglyceride, low density lipoprotein cholesterol (LDL-C), oxidized LDL-C, high density lipoprotein cholesterol (HDL-C), non-esterified fatty acid (NEFA), superoxide dismutase (SOD), catalase (CAT), methane dicarboxylic aldehyde (MDA), the expressions of SOD isoforms and glutathione peroxidase 1, as well as histopathological examination. In addition, generation of reactive oxygen species (ROS) was detected by 2′7′-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence. The results showed that UDCA alleviated renal ER stress-evoked cell death, oxidative stress, renal dysfunction, ROS production, upregulated the expression of Bcl-2 and suppressed Bax in vivo and in vitro. Hence, inhibition ER stress diminishes oxidative stress and exerts renoprotective effects.Key words diabetic nephropathy; podocyte; oxidative stress; reactive oxygen species Diabetic mellitus is a global health problem in which blood glucose is persistently elevated and generates a cascade of events in organ including kidney.1,2) The population with diabetes has been increasing worldwide and diabetic nephropathy (DN) now is a leading cause of end-stage renal failure.3)The mechanisms by which hyperglycemia contributes to kidney remain limited due to various factors modulate the plasma glucose in the body. The management of DN is based on the control of plasma glucose levels. 4) Reviewing number of studies demonstrates that reactive oxygen species (ROS), associated with increased plasma glucose, has been implicated in the pathogenesis of DN, which results in the over-production of extracellular matrix proteins, mitochondrial damage and glomeruli injury.5-8) Therefore, oxidative stress attenuation is an important pathway in DN prevention. 9)The endoplasmic reticulum (ER) regulates the folding of secretory and intracellular calcium.10) Excessive unfolded proteins in the lumen of ER produces stress and contributed to the disorder of intracellular signal transduction pathways. 11)A number of pathophysiological conditions are associated with ER stress included diabetes. ER stress is a key mediator of β...

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Section: )supporting

confidence: 78%

“…31) In our study, the level of renal MDA was increased in untreated diabetic mice compared to normal control group. UDCA treatment demonstrated its anti-lipid peroxidation activity.…”

Section: )supporting

confidence: 51%

Ursodeoxycholic Acid Ameliorated Diabetic Nephropathy by Attenuating Hyperglycemia-Mediated Oxidative Stress

Wang

Chen

et al. 2016

Biological & Pharmaceutical Bulletin

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“…Increases of IL-1 and IL-4 levels are induced by clinical and experimental diabetes [44,45]. The generation of ROS induced by hyperglycaemia plays a central role in the pathogenesis of diabetic neuropathy and brain oxidative injury [1] and these radicals can stimulate the production of cytokines [45,46]. It was also reported that diabetes-induced impairments in IL-1 signalling were linked to aberrant mitochondrial bioenergetics [42].…”

Section: Parametersmentioning

confidence: 99%

Melatonin and selenium reduce plasma cytokine and brain oxidative stress levels in diabetic rats

2015

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“…Two active steroidal lactones in Withania root extract are withaferin and withanolides (2). Some of therapeutics effects of Withania coagulans extract (WCE) have been reported by researchers (3)(4)(5). Upadhyay and et al reported antihyperglycemic effect of WCE in diabetic patients (1).…”

Section: Introductionmentioning

confidence: 99%

Withania coagulans Protects Striatum from Oxidative Damages Induced by Global Brain Ischemia in Rat

Mahmoudzadeh‐Sagheb

Heidari

Sarbishegi

2017

Jundishapur J Nat Pharm Prod

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Background: Withania coagulans is a traditional plant that possesses diverse pharmacological characteristics including antihyperglycemic, anti-hyperlipidemic, antitumor, anti-inflammatory, and antioxidant effects. Objectives: This study evaluated the effect of preischemic administration of Withania coagulans extract (WCE) on antioxidant status and histopathological alteration of the striatum region in rat model of brain global ischemia reperfusion (I/R). Methods: 48 male Wistar rats were divided into four groups (n = 12): Control, Sham operated (Sham), I/R, and WCE + I/R. The animals received WCE or distilled water by oral gavage for 30 days before I/R except the control group. I/R was induced by two common carotid obstructions for 30 minutes. Three days following I/R, the animals were sacrificed, the brain was removed, and striatum was dissected out. Antioxidant statuses such as superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activities, and malondialdehyde (MDA) level were measured and histological alteration was investigated in corpus striatum by Nissl and TUNEL staining. Results:The results showed that the CAT, SOD, and GPx activities decreased and MDA level increased in I/R group compare to the control group. Preischemic administration of WCE significantly decreased MDA level and increased antioxidant statuses in the striatum region compared to I/R group (P < 0.001). Histopathological analysis revealed that pretreatment with WCE markedly decreased necrotic and apoptotic neurons (P < 0.001) in striatum and reduced white matter injury induced by I/R. Conclusions: We concluded that WCE by its antioxidant and anti-inflammatory effects could modify ischemia reperfusion damage in rat striatum.

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Withania coagulansFruit Extract Reduces Oxidative Stress and Inflammation in Kidneys of Streptozotocin-Induced Diabetic Rats

Cited by 44 publications

References 29 publications

Ursodeoxycholic Acid Ameliorated Diabetic Nephropathy by Attenuating Hyperglycemia-Mediated Oxidative Stress

Ursodeoxycholic Acid Ameliorated Diabetic Nephropathy by Attenuating Hyperglycemia-Mediated Oxidative Stress

Melatonin and selenium reduce plasma cytokine and brain oxidative stress levels in diabetic rats

Withania coagulans Protects Striatum from Oxidative Damages Induced by Global Brain Ischemia in Rat

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