<i>Wolbachia</i>
            Influences the Maternal Transmission of the
            <i>gypsy</i>
            Endogenous Retrovirus in Drosophila melanogaster

Touret, Franck; Guiguen, François; Terzian, Christophe

doi:10.1128/mbio.01529-14

Cited by 25 publications

(26 citation statements)

References 39 publications

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“…Drosophila mauritiana females show both an increase in egg production as well as an increase in mitotic activity of germline stem cells associated with Wolbachia infection (Fast et al 2011). In D. melanogaster, Wolbachia infection reduces maternal transmission of the endogenous retrovirus gypsy (Touret et al 2014). The presence of Wolbachia also significantly affects protein abundance in D. melanogaster ovaries (Christensen et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Wolbachia Infection Associated with Increased Recombination in Drosophila

Singh

2017

Preprint

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Wolbachia is maternally-transmitted endosymbiotic bacteria that infects a large diversity of arthropod and nematode hosts. Some strains of Wolbachia are parasitic, manipulating host reproduction to benefit themselves, while other strains of Wolbachia exhibit obligate or facultative mutualisms with their host. The effects of Wolbachia on its host are many, though primarily relate to host immune and reproductive function. Here we test the hypothesis that Wolbachia infection alters the frequency of homologous recombination during meiosis. We use D. melanogaster as a model system, and survey recombination in eight Wolbachia-infected and Wolbachia-uninfected strains, controlling for genotype. We measure recombination in two intervals of the genome. Our results indicate that Wolbachia infection is associated with increased recombination in one genomic interval and not the other. The effect of Wolbachia infection on recombination is thus heterogenous across the genome. Our data also indicate a reproductive benefit of Wolbachia infection; infected females show higher fecundity than their uninfected genotypic controls. Given the prevalence of Wolbachia infection in natural populations, our findings suggest that Wolbachia infection is likely to contribute to recombination rate and fecundity variation among individuals in nature.

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Section: Discussionmentioning

confidence: 99%

Wolbachia Infection Associated with Increased Recombination in Drosophila

Singh

2017

Preprint

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“…We did not detect any statistically significant differences in mitochondria titer between infected and uninfected ovaries (Figure 1a,b). Other studies also found no effect of clearing Wolbachia infection on mitochondria titer in D. melanogaster ovaries (Touret, Guiguen, & Terzian, 2014) or in whole Leptopilina parasitoid wasps (Mouton, Henri, & Fleury, 2009). Second, we reasoned that if we reduce mitochondria populations that limit Wolbachia, we would expect to see an increase in Wolbachia populations.…”

Section: Discussionmentioning

confidence: 91%

Mitochondria and Wolbachia titers are positively correlated during maternal transmission

Henry

Newton

2018

Molecular Ecology

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Mothers provide their offspring with symbionts. Maternally transmitted, intracellular symbionts must disperse from mother to offspring with other cytoplasmic elements, like mitochondria. Here, we investigated how the intracellular symbiont Wolbachia interacts with mitochondria during maternal transmission. Mitochondria and Wolbachia may interact antagonistically and compete as each population tries to ensure its own evolutionary success. Alternatively, mitochondria and Wolbachia may cooperate as both benefit from ensuring the fitness of the mother. We characterized the relationship between mitochondria and Wolbachia titers in ovaries of Drosophila melanogaster. We found that mitochondria and Wolbachia titers are positively correlated in common laboratory genotypes of D. melanogaster. We attempted to perturb this covariation through the introduction of Wolbachia variants that colonize at different titers. We also attempted to perturb the covariation through manipulating the female reproductive tract to disrupt maternal transmission. Finally, we also attempted to disrupt the covariation by knocking down gene expression for two loci involved in mitochondrial metabolism: NADH dehydrogenase and a mitochondrial transporter. Overall, we find that mitochondria and Wolbachia titers are commonly positively correlated, but this positive covariation is disrupted at high titers of Wolbachia. Our results suggest that mitochondria and Wolbachia have likely evolved mechanisms to stably coexist, but the competitive dynamics change at high Wolbachia titers. We provide future directions to better understand how their interaction influences the maintenance of the symbiosis.

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“…For example, Factor I retrotransposon RNAs produced in nurse cells migrate to the oocyte nucleus [5–7] and, likewise, Gypsy I RNAs produced in follicle cells traverse the perivitelline membrane to access the oocyte [6, 8, 9]. …”

Section: Resultsmentioning

confidence: 99%

Retrotransposons Mimic Germ Plasm Determinants to Promote Transgenerational Inheritance

et al. 2017

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SUMMARY Retrotransposons are a pervasive class of mobile elements present in the genomes of virtually all forms of life [1, 2]. In metazoans these are preferentially active in the germline, which, in turn, mounts defenses that restrain their activity [3, 4]. Here we report that certain classes of retrotransposons insure transgenerational inheritance by invading presumptive germ cells before they are formed. Using sensitized Drosophila and zebrafish models, we found that diverse classes of retrotransposons migrate to the germ plasm, a specialized region of the oocyte that prefigures germ cells and specifies the germline of descendants in the fertilized egg. In Drosophila, we found evidence for a ‘stowaway’ model, whereby Tahre retroelements traffic to the germ plasm by mimicking oskar RNAs and engaging the Staufen-dependent active transport machinery. Consistent with this germ plasm determinants attracted retroelement RNAs, even when these components were ectopically positioned in bipolar oocytes. Likewise, vertebrate retrotransposons similarly migrated to the germ plasm in zebrafish oocytes. Together, these results suggest that germ plasm targeting represents a fitness strategy adopted by some retrotransposons to insure transgenerational propagation.

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Wolbachia Influences the Maternal Transmission of the gypsy Endogenous Retrovirus in Drosophila melanogaster

Cited by 25 publications

References 39 publications

Wolbachia Infection Associated with Increased Recombination in Drosophila

Wolbachia Infection Associated with Increased Recombination in Drosophila

Mitochondria and Wolbachia titers are positively correlated during maternal transmission

Retrotransposons Mimic Germ Plasm Determinants to Promote Transgenerational Inheritance

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