<i>Wolbachia</i> modifies thermal preference in <scp><i>Drosophila melanogaster</i></scp>

Truitt, Amy Michelle; Kapun, Martin; Kaur, Rupinder; Miller, Wolfgang J.

doi:10.1111/1462-2920.14347

Cited by 78 publications

(130 citation statements)

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“…But how has the ancestral Asian strain be replaced? Wolbachia acquisition and persistence in C. obscurior may be driven by selection on the strain that is better adapted to the local environment, perhaps analogous to Wolbachia -driven thermal preferences in Drosophila flies 49,50 . Resolving how w Cobs-BR replaced the putatively ancestral w Cobs-JP strain (which holds the power of CI) requires testing how mixed combinations of host and Wolbachia perform under varying ecological conditions.…”

Section: Discussionmentioning

confidence: 99%

Cytoplasmic incompatibility between Old and New World populations of a tramp ant

Ün

Schultner

Manzano-Marı́n

et al. 2020

Preprint

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34 35 Contributions 36 CÜ performed the crosses and qPCR 37 ES analyzed and visualized the data 38 AMM assembled Wolbachia genomes, performed comparative genomic analyses, 39 calculated the phylogeny and annotated CI genes 40 LVF conducted FISH analyses 41 BS revised the taxonomic status of the populations 42 JH acquired funding and reviewed and edited the manuscript 43 JO conceived and supervised the study 44 JO, ES and CÜ wrote the original draft of the manuscript 48As we enter the Anthropocene, the evolutionary dynamics of species will change 49 drastically, and as yet unpredictably, due to human activity. Already today, increases in 50 global human traffic have resulted in the rapid spread of species to new areas, leading to 51 the formation of geographically isolated populations. These go on to evolve in allopatry, 52 which can lead to reproductive isolation, and potentially, the formation of new species.53 Surprisingly, little is known about such eco-evolutionary processes in ants, even though 54 numerous invasive ant species are globally distributed in geographically isolated 55 populations. Here, we describe the first case of cytoplasmic incompatibility (CI) between 56 populations of a cosmotropic distributed tramp ant with Asian roots, Cardiocondyla 57 obscurior, which has acquired a novel Wolbachia strain in the New World. Our study 58 uncovers the first symbiont-induced mechanism of reproductive isolation in ants, 59 providing a novel perspective on the biology of globally distributed ants. 61Introduction 62 Ants are the most abundant group of insects on earth, and numerous ant species are 63 classified as highly invasive on a global scale. Their distribution is, on the one hand, 64 strongly facilitated by humans. This is evidenced, for instance, by the rapid worldwide 65 spread of the Argentine ant and the fire ant from their origin in South America 1,2 . On the 66 other hand, the particular biology of ants, characterized by reproductive division of labor 67 between highly fecund queens and sterile workers, allows the establishment of large 68 populations from just one founding propagule. Introduced populations thus go through a 69 genetic bottleneck, reducing diversity within the population while at the same time 70 increasing diversity between populations 3 . Such populations then evolve in allopatry, and 71 differences caused by genetic drift may be further amplified over time by selection, 72 eventually leading to reproductive isolation. Reproductive isolation, in turn, together with 73 the evolution of mating systems, underlies the formation of new species and, hence, the 74 vast diversity of life. By allowing ants (and other organisms) to establish allopatric 75 populations around the world, humans have inadvertently created a valuable scenario for 76 studying species evolution in real time. 78Reproductive isolation between populations that live in allopatry can evolve in the form of 79 post-mating mechanisms of reproductive isolation (PoMRI). Importantly, PoMRI can result 80 from Bateson-Dobzha...

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Section: Discussionmentioning

confidence: 99%

Cytoplasmic incompatibility between Old and New World populations of a tramp ant

Ün

Schultner

Manzano-Marı́n

et al. 2020

Preprint

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“…Our results indicate that the infection rate has propensity to decline over the last decade in D. melanogaster population in Uman'. Truit et al [24] have shown that infected flies prefer a lower range of temperature than uninfected ones. This behavioral adaptation is likely affecting the accuracy of the estimation of the link between temperature and infection frequencies.…”

Section: Discussionmentioning

confidence: 99%

Dynamics of Frequencies of Wolbachia Genotypes in Drosophila Melanogaster Population From Uman’ Under Influence of Climate Factors

Gora¹,

Serga²,

Maistrenko³

et al. 2019

Microbiology&Biotechnology

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Aim. To investigate the potential influence of climate parameters on the rate of Wolbachia genotypes frequencies in the population of D. melanogaster from Uman' for the last seven years. Methods. We have surveyed Wolbachia infection status for isofemale lines from Uman' collected during summer -fall 2013 and 2015-2017 using polymerase chain reaction (PCR) with primers specific to the 16S rRNA and wsp (Wolbachia surface protein) genes. To determine wMel and wMelCS genotypes of Wolbachia, we have conducted PCR using polymorphic markers for the presence of the insertion sequence IS5 in WD1310 locus and the copy number of minisatellite repeats in VNTR-141 locus. Infection status of flies for 2011, 2012 and 2014 years was included from our previous studies. Data of climate factors (average seasonal temperature, dew point, and precipitation) for winter and summer of each year were obtained from weather database. Statistical analysis of Wolbachia genotypes-climate factors interaction was carried out in R version 3.5.0 using multiple linear model regression. Results. We have observed the decline of Wolbachia presence in Uman' locality and prevalence of wMel bacteria variant. Moreover, our study documented the low persistence of rare wMelCS genotype through seven years in Uman'. wMelCS frequency has been driven by the combined influence of the average temperature and humidity in summer (p = 0.03662, R^2 = 0.9995). Conclusions. The climate variability affects frequency of wMelCS genotype of Wolbachia in the D. melanogaster natural population from Uman'.

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“…The persistence of Wolbachia, for example, is negatively affected by host fitness via temperature as well as by other stress factors (e.g. [66][67][68]). Thus, the more stressful conditions of the dry season may reduce the overall fitness of the fly and consequently the Spiroplasma infection densities.…”

Section: Geographic Origin and Seasonalitymentioning

confidence: 99%

Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda

Schneider¹,

Saarman

Onyango³

et al. 2019

Preprint

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DIS) ¶ These authors contributed equally to this work. AbstractTsetse flies (Glossina spp.) are vectors of parasitic trypanosomes, which cause human (HAT) and animal African trypanosomiasis (AAT) in sub-Saharan Africa. In Uganda, Glossina fuscipes fuscipes (Gff) is the main vector of HAT, where it transmits Gambiense disease in the northwest and Rhodesiense disease in central, southeast and western regions. Endosymbionts can influence transmission efficiency of parasites through their insect vectors via conferring a protective effect against the parasite. It is known that the bacterium Spiroplasma is capable of protecting its Drosophila host from infection with a parasitic nematode. This endosymbiont can also impact its host's population structure via altering host reproductive traits. Here, we used field collections across 26 different Gff sampling sites in northern and western Uganda to investigate the association of Spiroplasma with geographic origin, seasonal conditions, Gff genetic background and sex, and trypanosome infection status. We also investigated the influence of Spiroplasma on Gff vector competence to trypanosome infections under laboratory conditions. Generalized linear models (GLM) showed that Spiroplasma probability was correlated with the geographic origin of Gff host and with the season of collection, with higher prevalence found in flies within the Albert Nile (0.42 vs 0.16) and Achwa River (0.36 vs 0.08) watersheds and with higher prevalence detected in flies collected in the intermediate than wet season. In contrast, there was no significant correlation of Spiroplasma prevalence with Gff host genetic background or sex once geographic origin was accounted for in generalized linear models. Additionally, we found a potential negative correlation of Spiroplasma with trypanosome infection, with only 2% of Spiroplasma infected flies harboring trypanosome co-infections. We also found that in a laboratory line of Gff, parasitic trypanosomes are less likely to colonize the midgut in individuals that harbor Spiroplasma infection. These results indicate that Spiroplasma infections in tsetse may be maintained by not only maternal but also via horizontal transmission routes, and Spiroplasma infections may also have important effects on trypanosome transmission efficiency of the host tsetse. Potential functional effects of Spiroplasma infection in Gff could have impacts on vector control approaches to reduce trypanosome infections. Author SummaryWe investigated the association of symbiotic Spiroplasma with the tsetse fly host Glossina fuscipes fuscipes (Gff) to assess if Spiroplasma infections are correlated withGff genetic background, geography, or season and its interaction with trypanosome parasites. We analyzed distribution and prevalence of Spiroplasma infections across different Gff sampling sites in northern and western Uganda, and found that the symbiont is unevenly distributed and infections have not reached fixation within these sampling sites. We tested for associations with geographic origin ...

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Wolbachia modifies thermal preference in Drosophila melanogaster

Cited by 78 publications

References 78 publications

Cytoplasmic incompatibility between Old and New World populations of a tramp ant

Cytoplasmic incompatibility between Old and New World populations of a tramp ant

Dynamics of Frequencies of Wolbachia Genotypes in Drosophila Melanogaster Population From Uman’ Under Influence of Climate Factors

Spatio-temporal distribution of Spiroplasma infections in the tsetse fly (Glossina fuscipes fuscipes) in northern Uganda

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