<i>Wolbachia</i> modulates prevalence and viral load of Culex pipiens densoviruses in natural populations

Altinli, Mine; Lequime, Sébastian; Atyame, Célestine M.; Justy, Fabienne; Weill, Mylène; Sicard, Mathieu

doi:10.1111/mec.15609

Cited by 16 publications

(9 citation statements)

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“…Indeed, a specific strain of Wolbachia ( w Pip-IV) was associated with higher CpDV loads in ovaries of laboratory colonies ( Altinli et al., 2019b ) and a higher prevalence in nature compared to another w Pip type (i.e. w Pip-I) ( Altinli et al., 2020 ). w Pip induces cytoplasmic incompatibility, a conditional sterility in crosses between females and males infected with incompatible Wolbachia strains in Cx.…”

Section: Symbiont-symbiont Interactionsmentioning

confidence: 99%

Symbiotic Interactions Between Mosquitoes and Mosquito Viruses

Altinli

Schnettler

Sicard

2021

Front. Cell. Infect. Microbiol.

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Mosquitoes not only transmit human and veterinary pathogens called arboviruses (arthropod-borne viruses) but also harbor mosquito-associated insect-specific viruses (mosquito viruses) that cannot infect vertebrates. In the past, studies investigating mosquito viruses mainly focused on highly pathogenic interactions that were easier to detect than those without visible symptoms. However, the recent advances in viral metagenomics have highlighted the abundance and diversity of viruses which do not generate mass mortality in host populations. Over the last decade, this has facilitated the rapid growth of virus discovery in mosquitoes. The circumstances around the discovery of mosquito viruses greatly affected how they have been studied so far. While earlier research mainly focused on the pathogenesis caused by DNA and some double-stranded RNA viruses during larval stages, more recently discovered single-stranded RNA mosquito viruses were heavily studied for their putative interference with arboviruses in female adults. Thus, many aspects of mosquito virus interactions with their hosts and host-microbiota are still unknown. In this context, considering mosquito viruses as endosymbionts can help to identify novel research areas, in particular in relation to their long-term interactions with their hosts (e.g. relationships during all life stages, the stability of the associations at evolutionary scales, transmission routes and virulence evolution) and the possible context-dependent range of interactions (i.e. beneficial to antagonistic). Here, we review the symbiotic interactions of mosquito viruses considering different aspects of their ecology, such as transmission, host specificity, host immune system and interactions with other symbionts within the host cellular arena. Finally, we highlight related research gaps in mosquito virus research.

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Section: Symbiont-symbiont Interactionsmentioning

confidence: 99%

Symbiotic Interactions Between Mosquitoes and Mosquito Viruses

Altinli

Schnettler

Sicard

2021

Front. Cell. Infect. Microbiol.

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“…Indeed, Chaturvedi et al (2018) found that populations along the eastern slope of the Sierra Nevada Mountains were more similar to each other genetically relative to populations in other parts of the range, suggesting a common lineage in this area. Alternatively, JcDV prevalence could be maintained by other site‐dependent factors such as interactions with endosymbionts (e.g., Wolbachia , Altinli et al, 2020; Shastry et al, 2022), or abiotic factors (Carlson et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Exploring spatial and temporal patterns of viral infection across populations of the Melissa blue butterfly

McKeegan,

Muchoney,

Teglas

et al. 2023

Ecological Entomology

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Identifying patterns of pathogen infection in natural systems is crucial to understanding mechanisms of host–pathogen interactions. In this study, we explored how Junonia coenia densovirus (JcDV) infection varies over space and time in populations of the Melissa blue butterfly (Lycaeides melissa: Lycaenidae) using two different host plants. Collections of L. melissa adults from multiple populations and years, along with host plant tissue and community samples of arthropods found on host plants, were screened to determine JcDV prevalence and load. Additionally, we sampled at multiple time points within a single L. melissa flight season to investigate intra‐annual variation in infection patterns. We found population‐specific variation in viral prevalence of L. melissa across collection years, with historical samples potentially having higher viral prevalence than contemporary samples, although host plant diet was not informative for these patterns. Patterns of infection across multiple generations within a flight season showed that late‐season samples had a higher proportion of JcDV‐positive individuals, suggesting an accumulation of virus over the season. Sequence data from a segment of the JcDV capsid gene showed a lack of viral genetic diversity between L. melissa collected from different localities, and little to no viral particles were found in the surrounding environment. Our discovery of temporal variation in infection suggests that multiple sampling efforts must be made when describing pathogen prevalence in multivoltine hosts. Our findings represent an important first step towards further exploration of the ecological factors mediating disease prevalence and host‐specific variability of infection in wild insect populations.

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“…Several reports have discussed and well documented the negative effect of Wolbachia on RNA viruses in different insect models such as mosquitoes and Drosophila [73][74][75], although there have also been reports about Wolbachia enhancement of both RNA and DNA viruses [76][77][78]. It is worth mentioning that the negative correlation of Wolbachia with GpSGHV was observed only when Wolbachia density was high as the results show the absence of high density (> 10 3.7 ) GpSGHV infection with high-density Wolbachia infection (> 10 7.5 ).…”

Section: Discussionmentioning

confidence: 99%

Interactions between Glossina pallidipes salivary gland hypertrophy virus and tsetse endosymbionts in wild tsetse populations

et al. 2022

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Background Tsetse control is considered an effective and sustainable tactic for the control of cyclically transmitted trypanosomosis in the absence of effective vaccines and inexpensive, effective drugs. The sterile insect technique (SIT) is currently used to eliminate tsetse fly populations in an area-wide integrated pest management (AW-IPM) context in Senegal. For SIT, tsetse mass rearing is a major milestone that associated microbes can influence. Tsetse flies can be infected with microorganisms, including the primary and obligate Wigglesworthia glossinidia, the commensal Sodalis glossinidius, and Wolbachia pipientis. In addition, tsetse populations often carry a pathogenic DNA virus, the Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) that hinders tsetse fertility and fecundity. Interactions between symbionts and pathogens might affect the performance of the insect host. Methods In the present study, we assessed associations of GpSGHV and tsetse endosymbionts under field conditions to decipher the possible bidirectional interactions in different Glossina species. We determined the co-infection pattern of GpSGHV and Wolbachia in natural tsetse populations. We further analyzed the interaction of both Wolbachia and GpSGHV infections with Sodalis and Wigglesworthia density using qPCR. Results The results indicated that the co-infection of GpSGHV and Wolbachia was most prevalent in Glossina austeni and Glossina morsitans morsitans, with an explicit significant negative correlation between GpSGHV and Wigglesworthia density. GpSGHV infection levels > 103.31 seem to be absent when Wolbachia infection is present at high density (> 107.36), suggesting a potential protective role of Wolbachia against GpSGHV. Conclusion The result indicates that Wolbachia infection might interact (with an undefined mechanism) antagonistically with SGHV infection protecting tsetse fly against GpSGHV, and the interactions between the tsetse host and its associated microbes are dynamic and likely species specific; significant differences may exist between laboratory and field conditions. Graphical Abstract

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Wolbachia modulates prevalence and viral load of Culex pipiens densoviruses in natural populations

Abstract: This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

Cited by 16 publications

References 95 publications

Symbiotic Interactions Between Mosquitoes and Mosquito Viruses

Symbiotic Interactions Between Mosquitoes and Mosquito Viruses

Exploring spatial and temporal patterns of viral infection across populations of the Melissa blue butterfly

Interactions between Glossina pallidipes salivary gland hypertrophy virus and tsetse endosymbionts in wild tsetse populations

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