<i>XsFAD2</i>
 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in <i>Xanthoceras sorbifolia</i>
 seeds

Guo, Hongwei; Li, Qiu-Qi; Wang, Tingting; Hu, Qing; Deng, Wenhong; Xia, Xinli; Gao, Hongbo

doi:10.1002/jsfa.6273

Cited by 16 publications

(13 citation statements)

References 28 publications

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“…The expression level of the FAD2-2 gene rapidly increased from 40 to 54/68 daa in the two lines and then decreased at the mature stage (Fig. 9j), which correlated well with the changes in linoleic acid and the total FA content in developing yellowhorn seeds [8]. Extensive research suggests that FA biosynthesis in higher plants may be an important regulatory step in oil accumulation [51,52].…”

Section: Mirnas and Their Targets Are Related To Yellowhorn Lipid Biosupporting

confidence: 57%

“…Yellowhorn research over the past decade has focused on oil extraction, FA composition, and the use of seed oil as a biodiesel [5][6][7]. Yellowhorn lipid biosynthesis metabolic pathways have been the subject of several studies, which identi ed some key genes associated with oil accumulation [8,9]. For example, two novel diacylglycerol acyltransferase (XsDGAT1 and XsDGAT2) yellowhorn genes were found to control its seed oil content [10].…”

Section: Introductionmentioning

confidence: 99%

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Small RNA proﬁling for identification of microRNAs involved in regulation of seed development and lipid biosynthesis in yellowhorn

Wang

Ruan

Bao

et al. 2021

Preprint

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Background Yellowhorn (Xanthoceras sorbifolium), an endemic woody oil-bearing tree, has become economically important and is widely cultivated in northern China for bioactive oil production. However, the regulatory mechanisms of seed development and lipid biosynthesis affecting oil production in yellowhorn are still elusive. MicroRNAs (miRNAs) play crucial roles in diverse aspects of biological and metabolic processes in seeds, especially in seed development and lipid metabolism. It is still unknown how the miRNAs regulate the seed development and lipid biosynthesis in yellowhorn. Results Here, based on investigations of differences in the seed growth tendency and embryo oil content between high-oil-content and low-oil-content lines, we constructed small RNA libraries from yellowhorn embryos at four seed development stages of the two lines and then profiled small RNA expression using high-throughput sequencing. A total of 249 known miRNAs from 46 families and 88 novel miRNAs were identified. Furthermore, by pairwise comparisons among the four seed development stages in each line, we found that 64 miRNAs (53 known and 11 novel miRNAs) were differentially expressed in the two lines. Across the two lines, 15, 11, 10, and 7 differentially expressed miRNAs were detected at 40, 54, 68, and 81 days after anthesis, respectively. Bioinformatic analysis was used to predict a total of 2,654 target genes for 141 differentially expressed miRNAs (120 known and 21 novel miRNAs). Most of these genes were involved in the fatty acid biosynthetic process, regulation of transcription, nucleus, and response to auxin. Using quantitative real-time PCR and an integrated analysis of miRNA and mRNA expression, miRNA-target regulatory modules that may be involved in yellowhorn seed size, weight, and lipid biosynthesis were identified, such as miR172b-ARF2 (auxin response factor 2), miR7760-p3_1-AGL61 (AGAMOUS-LIKE 61), miR319p_1-FAD2-2 (omega-6 fatty acid desaturase 2–2), and miR5647-p3_1-DGAT1 (diacylglycerol acyltransferase 1). Conclusions This study provides new insights into the important regulatory roles of miRNAs in the seed development and lipid biosynthesis in yellowhorn. Our results will be valuable for dissecting the post-transcriptional and transcriptional regulation of seed development and lipid biosynthesis, as well as improving yellowhorn in northern China.

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Section: Mirnas and Their Targets Are Related To Yellowhorn Lipid Biosupporting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

Small RNA proﬁling for identification of microRNAs involved in regulation of seed development and lipid biosynthesis in yellowhorn

Wang

Ruan

Bao

et al. 2021

Preprint

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“…Yeast has been shown to be an ideal model system for identifying the function of ER-located desaturases, including FAD2 and FAD3 [ 17 , 71 ], but it was not suitable for heterologous expression of plastidial desaturases (e.g. FAD6/7/8) due to their requirements for electron transport chains from the chloroplast [ 16 ].…”

Section: Resultsmentioning

confidence: 99%

Omega-3 fatty acid desaturase gene family from two ω-3 sources, Salvia hispanica and Perilla frutescens: Cloning, characterization and expression

et al. 2018

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Omega-3 fatty acid desaturase (ω-3 FAD, D15D) is a key enzyme for α-linolenic acid (ALA) biosynthesis. Both chia (Salvia hispanica) and perilla (Perilla frutescens) contain high levels of ALA in seeds. In this study, the ω-3 FAD gene family was systematically and comparatively cloned from chia and perilla. Perilla FAD3, FAD7, FAD8 and chia FAD7 are encoded by single-copy (but heterozygous) genes, while chia FAD3 is encoded by 2 distinct genes. Only 1 chia FAD8 sequence was isolated. In these genes, there are 1 to 6 transcription start sites, 1 to 8 poly(A) tailing sites, and 7 introns. The 5’UTRs of PfFAD8a/b contain 1 to 2 purine-stretches and 2 pyrimidine-stretches. An alternative splice variant of ShFAD7a/b comprises a 5’UTR intron. Their encoded proteins harbor an FA_desaturase conserved domain together with 4 trans-membrane helices and 3 histidine boxes. Phylogenetic analysis validated their identity of dicot microsomal or plastidial ω-3 FAD proteins, and revealed some important evolutionary features of plant ω-3 FAD genes such as convergent evolution across different phylums, single-copy status in algae, and duplication events in certain taxa. The qRT-PCR assay showed that the ω-3 FAD genes of two species were expressed at different levels in various organs, and they also responded to multiple stress treatments. The functionality of the ShFAD3 and PfFAD3 enzymes was confirmed by yeast expression. The systemic molecular and functional features of the ω-3 FAD gene family from chia and perilla revealed in this study will facilitate their use in future studies on genetic improvement of ALA traits in oilseed crops.

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“…To achieve the maximum amount of oil yield, a better understanding of the mechanisms of oil biosynthesis and the specific molecular pathways involved is necessary. Several studies have attempted to examine yellow horn oil biosynthesis metabolic pathways and have identified some genes found to be associated with oil accumulation [ 4 , 5 , 6 ]. One study found that the expression of two novel diacylglycerol acyltransferase genes ( XsDGAT1 and XsDGAT2 ) in transgenic Arabidopsis led to triacylglycerol (TAG) assembly and increased seed oil production [ 4 ]; with the expression patterns of both genes also correlated with oil accumulation in developing yellow horn embryos, presumably via TAG assembly.…”

Section: Introductionmentioning

confidence: 99%

Comparative RNA-Seq Analysis of High- and Low-Oil Yellow Horn During Embryonic Development

Wang

Ruan

Liu

et al. 2018

IJMS

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Yellow horn (Xanthoceras sorbifolium Bunge) is an endemic oil-rich shrub that has been widely cultivated in northern China for bioactive oil production. However, little is known regarding the molecular mechanisms that contribute to oil content in yellow horn. Herein, we measured the oil contents of high- and low-oil yellow horn embryo tissues at four developmental stages and investigated the global gene expression profiles through RNA-seq. The results found that at 40, 54, 68, and 81 days after anthesis, a total of 762, 664, 599, and 124 genes, respectively, were significantly differentially expressed between the high- and low-oil lines. Gene ontology (GO) enrichment analysis revealed some critical GO terms related to oil accumulation, including acyl-[acyl-carrier-protein] desaturase activity, pyruvate kinase activity, acetyl-CoA carboxylase activity, and seed oil body biogenesis. The identified differentially expressed genes also included several transcription factors, such as, AP2-EREBP family members, B3 domain proteins and C2C2-Dof proteins. Several genes involved in fatty acid (FA) biosynthesis, glycolysis/gluconeogenesis, and pyruvate metabolism were also up-regulated in the high-oil line at different developmental stages. Our findings indicate that the higher oil accumulation in high-oil yellow horn could be mostly driven by increased FA biosynthesis and carbon supply, i.e. a source effect.

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XsFAD2 gene encodes the enzyme responsible for the high linoleic acid content in oil accumulated in Xanthoceras sorbifolia seeds

Abstract: Results suggested that XsFAD2 is responsible for the high linoleic acid content in X. sorbifolia seed oil. This study provides insight on the regulation mechanism of fatty acid synthesis in X. sorbifolia seeds and a valuable gene for improving the oil quality in oilseed trees.

Cited by 16 publications

References 28 publications

Small RNA proﬁling for identification of microRNAs involved in regulation of seed development and lipid biosynthesis in yellowhorn

Small RNA proﬁling for identification of microRNAs involved in regulation of seed development and lipid biosynthesis in yellowhorn

Omega-3 fatty acid desaturase gene family from two ω-3 sources, Salvia hispanica and Perilla frutescens: Cloning, characterization and expression

Comparative RNA-Seq Analysis of High- and Low-Oil Yellow Horn During Embryonic Development

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