<i>XTbx1</i> is a transcriptional activator involved in head and pharyngeal arch development in <i>Xenopus laevis</i>

Ataliotis, Paris; Ivins, Sarah; Mohun, Timothy J.; Scambler, Peter J.

doi:10.1002/dvdy.20276

Cited by 52 publications

(42 citation statements)

References 66 publications

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“…Conversely, overexpression of Tbx1 in the outflow tract increases cell number again arguing that, as changes in apoptosis have not been found, that one of the key functions of Tbx1 is to regulate cell proliferation. It should, however, be noted that a decrease in mesodermal proliferation has not always been observed after loss of Tbx1 function Ataliotis et al, 2005).…”

Section: Discussionmentioning

confidence: 97%

“…In its absence, the second arch muscles fail to develop while the mandibular or first arch muscles are hypoplastic and sporadic (Kelly et al, 2004). In the developing head, Tbx1 is expressed in both the endoderm and mesoderm and, therefore, myogenic defects may be a consequence of defective endoderm/ectoderm signaling (Vitelli et al, 2002b;Hu et al, 2004;Ataliotis et al, 2005, Zhang et al, 2005. To analyze whether Tbx1 is intrinsically required in the mesoderm, we performed micromass assays, cell cultures generated by plating single cell suspensions made from the mesoderm and neural crest-derived ectomesenchyme of branchial arch 1, at high density.…”

Section: Tbx1 Is Essential For the Development Of Craniofacial And LImentioning

confidence: 99%

“…The viruses were generated using a Xenopus Tbx1 cDNA (accession no. AF526274; Ataliotis et al, 2005). All viruses were made by cloning by means of pSLAX plasmid and subcloning of the Cla-Cla fragment containing the sequence of interest into the RCAS(BP) retroviral vector.…”

Section: Production Of Retrovirusesmentioning

confidence: 99%

“…Tbx1 is expressed in the endoderm and mesoderm of the branchial arches, and this expression pattern is conserved between mice, chicks, Xenopus, zebrafish, and lamprey (Chapman et al, 1996;Garg et al, 2001;Lindsay et al, 2001;Sauka-Spengler et al, 2002;Kochilas et al, 2003;Piotrowski et al, 2003;Ataliotis et al, 2005). In addition, expression of Tbx1 is also found in the otic vesicle and surrounding mesenchyme, where it is necessary for morphogenesis of the inner ear and the ultimate development of the cochlea and vestibular apparatus (Vitelli et al, 2003;Raft et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…It is unclear whether the myogenic defects in Tbx1 Ϫ/Ϫ mouse mutants or after injection of a dominant-negative Tbx1 construct into Xenopus embryos are due to abnormalities in the mesoderm, from which the muscles are derived, or are secondary due to defects in the endoderm such as the loss of Fgf8 signaling (Kelly et al, 2004;Ataliotis et al, 2005;Smith et al, 2005). Arguments for an intrinsic role include data showing that the ascidian T-box gene As-T2 is necessary and sufficient to induce myogenic differentiation and there is a functional T-box binding site in the Xenopus Myf5 promoter (Mitani et al, 2001;Lin et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

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Tbx1 regulation of myogenic differentiation in the limb and cranial mesoderm

Dastjerdi

Robson

Walker

et al. 2006

Developmental Dynamics

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The T-box transcription factor Tbx1 has been implicated in DiGeorge syndrome, the most frequent syndrome due to a chromosomal deletion. Gene inactivation of Tbx1 in mice results in craniofacial and branchial arch defects, including myogenic defects in the first and second branchial arches. A T-box binding site has been identified in the Xenopus Myf5 promoter, and in other species, T-box genes have been implicated in myogenic fate. Here we analyze Tbx1 expression in the developing chick embryo relating its expression to the onset of myogenic differentiation and cellular fate within the craniofacial mesoderm. We show that Tbx1 is expressed before capsulin, the first known marker of branchial arch 1 and 2 muscles. We also show that, as in the mouse, Tbx1 is expressed in endothelial cells, another mesodermal derivative, and, therefore, Tbx1 alone cannot specify the myogenic lineage. In addition, Tbx1 expression was identified in both chick and mouse limb myogenic cells, initially being restricted to the dorsal muscle mass, but in contrast, to the head, here Tbx1 is expressed after the onset of myogenic commitment. Functional studies revealed that loss of Tbx1 function reduces the number of myocytes in the head and limb, whereas increasing Tbx1 activity has the converse effect. Finally, analysis of the Tbx1-mesoderm-specific knockout mouse demonstrated the cell autonomous requirement for Tbx1 during myocyte development in the cranial mesoderm. Developmental Dynamics 236:353-363, 2007.

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Section: Discussionmentioning

confidence: 97%

Section: Tbx1 Is Essential For the Development Of Craniofacial And LImentioning

confidence: 99%

Section: Production Of Retrovirusesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Tbx1 regulation of myogenic differentiation in the limb and cranial mesoderm

Dastjerdi

Robson

Walker

et al. 2006

Developmental Dynamics

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Xenopus: An emerging model for studying congenital heart disease

Kaltenbrun

Tandon

Amin

et al. 2011

Birth Defects Research

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Congenital heart defects affect nearly 1% of all newborns and are a significant cause of infant death. Clinical studies have identified a number of congenital heart syndromes associated with mutations in genes that are involved in the complex process of cardiogenesis. The African clawed frog, Xenopus, has been instrumental in studies of vertebrate heart development and provides a valuable tool to investigate the molecular mechanisms underlying human congenital heart diseases. In this review, we discuss the methodologies that make Xenopus an ideal model system to investigate heart development and disease. We also outline congenital heart conditions linked to cardiac genes that have been well-studied in Xenopus and describe some emerging technologies that will further aid in the study of these complex syndromes.

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Evolutionary and developmental origins of the cardiac neural crest: Building a divided outflow tract

Keyte

Alonzo-Johnsen

Hutson

2014

Birth Defects Research Pt C

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The cardiac neural crest cells (CNCCs) have played an important role in the evolution and development of the vertebrate cardiovascular system: from reinforcement of the developing aortic arch arteries early in vertebrate evolution, to later orchestration of aortic arch artery remodeling into the great arteries of the heart, and finally outflow tract septation in amniotes. A critical element necessary for the evolutionary advent of outflow tract septation was the co-evolution of the cardiac neural crest cells with the second heart field. This review highlights the major transitions in vertebrate circulatory evolution, explores the evolutionary developmental origins of the CNCCs from the third stream cranial neural crest, and explores candidate signaling pathways in CNCC and outflow tract evolution drawn from our knowledge of DiGeorge Syndrome.

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XTbx1 is a transcriptional activator involved in head and pharyngeal arch development in Xenopus laevis

Cited by 52 publications

References 66 publications

Tbx1 regulation of myogenic differentiation in the limb and cranial mesoderm

Tbx1 regulation of myogenic differentiation in the limb and cranial mesoderm

Xenopus: An emerging model for studying congenital heart disease

Evolutionary and developmental origins of the cardiac neural crest: Building a divided outflow tract

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