<i>Yarrowia lipolytica</i>and Its Multiple Applications in the Biotechnological Industry

Gonçalves, Flávio de Oliveira; Colen, Gecernir; Takahashi, Jacqueline A.

doi:10.1155/2014/476207

Cited by 142 publications

(79 citation statements)

References 93 publications

(206 reference statements)

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“…The metabolism of glycerol to citric acid [42] and lipogenesis pathway [43] in Y. lipolytica has been studied before. Glycerol is assimilated into the cell via facilitated diffusion and is subsequently phosphorylated [41].…”

Section: Discussionmentioning

confidence: 99%

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Abghari

Chen

2017

Fermentation

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Increasing demand for plant oil for food, feed, and fuel production has led to food-fuel competition, higher plant lipid cost, and more need for agricultural land. On the other hand, the growing global production of biodiesel has increased the production of glycerol as a by-product. Efficient utilization of this by-product can reduce biodiesel production costs. We engineered Yarrowia lipolytica (Y. lipolytica) at various metabolic levels of lipid biosynthesis, degradation, and regulation for enhanced lipid and citric acid production. We used a one-step double gene knock-in and site-specific gene knock-out strategy. The resulting final strain combines the overexpression of homologous DGA1 and DGA2 in a POX-deleted background, and deletion of the SNF1 lipid regulator. This increased lipid and citric acid production in the strain under nitrogen-limiting conditions (C/N molar ratio of 60). The engineered strain constitutively accumulated lipid at a titer of more than 4.8 g/L with a lipid content of 53% of dry cell weight (DCW). The secreted citric acid reached a yield of 0.75 g/g (up to~45 g/L) from pure glycerol in 3 days of batch fermentation using a 1-L bioreactor. This yeast cell factory was capable of simultaneous lipid accumulation and citric acid secretion. It can be used in fed-batch or continuous bioprocessing for citric acid recovery from the supernatant, along with lipid extraction from the harvested biomass.

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Section: Discussionmentioning

confidence: 99%

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Abghari

Chen

2017

Fermentation

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“…Y. lipolytica is a yeast often isolated from hydrophobic substrates, rich in alkanes and fats, for example, cheese, yoghurt, soy sauce, meat, and shrimp salad, and is used in many industrial processes to obtain various products, such as citric and isocitric acids, γ-decalactone, lipase, and for its ability to produce and store lipids (Gonçalves, Colen, & Takahashi, 2014). Furthermore, it has been demonstrated that some Y. lipolytica strains are able to reduce pollution in olive mill wastewaters (Gonçalves, Lopes, Ferreira, & Belo, 2009).…”

Section: Discussionmentioning

confidence: 99%

Selection of yeasts for their anti‐mold activity and prospective use in table olive fermentation

Caridi

Panebianco

Scibetta

et al. 2019

J Food Process Preserv

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The growth of molds represents a major problem during table olive fermentation. Molds are recognized as spoilage agents and may reduce the product safety for their ability to produce mycotoxins. Yeasts, instead, are usually found in table olive processing and are generally considered desirable microorganisms for their technological properties. In the present study, a model system to select anti‐mold yeasts usable as protective adjunct cultures in table olive fermentation was developed. Two hundred and ninety‐nine strains of yeasts, isolated from cheese, olive, vinegar, and wine, were tested in vitro for their growth characteristics, fermentative activity, salt tolerance, and antagonistic activity against 10 mold strains isolated from table olives and olive brines. Experimental steps led to the selection of a strain of Yarrowia lipolytica exhibiting all the previously listed characteristics. Practical applications A strain of Yarrowia lipolytica was selected because of its high potentiality as adjunct culture in table olive fermentation to contrast mold growth and increase the product quality and safety. The experimental model system developed in this study may be easily applied to select yeasts usable as adjunct cultures in table olive fermentation in order to contrast mold growth and increase the product safety. These outcomes showed how the use of selected yeasts constitutes a promising way to control mold growth during table olive fermentation.

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“…Interestingly, genus Yarrowia, which contains a single-species Yarrowia lipolytica was present in high numbers in one of the samples. The species has attracted a lot of interest because of its very high biotechnological potential, especially due to its lipid metabolism abilities (Gonçalves et al, 2014), which are likely also useful for survival in the ecosystem of the dishwasher.…”

Section: Discussionmentioning

confidence: 99%

Who lives in our dishwasher? Preliminar results of fungal metagenomic analysis of household dishwashers

Koren

Kovač

Toplak

2015

AAS

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Who lives in our dishwasher? Preliminar results of fungal metagenomic analysis of household dishwashersIn the last few years the advances in molecular biological methods, especially the development of next generation sequencing, have drastically changed and improved our view of microbial world. Progress in new molecular techniques enables us to overcome potential disadvantages of traditional microbiological techniques in fungal community identifications. It also enables us to evaluate the richness of fungal populations more efficiently and reliably. In the present study, we used the Ion Torrent PGM next generation sequencing platform to analyse fungi present in ordinary household dishwashers. The identification was based on massive parallel sequencing of the D2 LSU rRNA amplicon. The analysis revealed rich and diverse fungal communities present in our dishwashers. Interpretation of the results was based on previously published research by Zalar et al. (2011). The results of our study confirmed that the new technology in many ways surpasses classical methods used in fungal analysis by offering quicker, reliable, more sensitive and inexpensive high-throughput identification of microorganisms in entire communities.Key words: molecular biology / molecular techniques / fungi / metagenomics / next generation sequencing / Ion Torrent PGM / household dishwashers Kdo živi v našem pomivalnem stroju? Preliminarni rezultati metagenomske analize gliv v gospodinjskih pomivalnih strojih Na področju metagenomike je napredek molekularno bioloških metod, predvsem razvoj naslednje generacije sekvenciranja, dramatično spremenil in razširil pogled na mikrobni svet. Napredek novih molekularnih tehnik nam omogoča premagovanje pomanjkljivosti tradicionalnih mikrobioloških tehnik, predvsem pri identifikacij populacij gliv. Z novim pristopom dobimo učinkovitejšo in zanesljivejšo ocenitev števila vrst gliv v določenih populacijah. V naši raziskavi smo uporabili tehnologijo naslednje generacije sekveniranja Ion Torrent za analizo prisotnosti gliv v gospodinjskih pomivalnih strojih. Identifikacija je temeljila na masivni paralelni določitvi nukleotidnega zaporedja podenote D2 LSU glivnega gena rRNA. S končno analizo smo potrdili bogate in raznolike skupnosti gliv v naših pomivalnih strojih, interpretacija rezultatov pa je temeljila na že objavljenih predhodnih raziskavah Zalarjeve in sod. (2011). Rezultati naše raziskave so potrdili, da nova tehnologija na mnogih področjih presega klasične mikrobiološke metode, ki se uporabljajo pri analizi skupnosti gliv in ponuja hitrejšo, zanesljivejšo, občutljivejšo ter cenejšo identifikacijo mikroorganizmov v skupnosti.Ključne besede: molekularna biologija / molekularne tehnike / glive / metagenomika / naslednja generacija sekveniranja / Ion Torrent PGM / gospodinjski pomivalni stroji

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Yarrowia lipolyticaand Its Multiple Applications in the Biotechnological Industry

Cited by 142 publications

References 93 publications

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Engineering Yarrowia lipolytica for Enhanced Production of Lipid and Citric Acid

Selection of yeasts for their anti‐mold activity and prospective use in table olive fermentation

Who lives in our dishwasher? Preliminar results of fungal metagenomic analysis of household dishwashers

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