<i>YUCCA</i>-mediated auxin biogenesis is required for cell fate transition occurring during<i>de novo</i>root organogenesis in Arabidopsis

Chen, Lyuqin; Tong, Jianhua; Xiao, Langtao; Ruan, Ying; Liu, Jingchun; Zeng, Minhuan; Huang, Hai; Wang, Jia-Wei; Xu, Lin

doi:10.1093/jxb/erw213

Cited by 168 publications

(166 citation statements)

References 32 publications

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“…YUCCAmediated auxin biosynthesis was shown to be ubiquitously enhanced in the mesophyll of leaf blades shortly after wounding (Chen et al, 2016). During rooting of whole leaves, we detected Pro YUC9 :GUS-enriched expression in leaf mesophyll cells at 12 hae, which progressively decreased at later time points (Supplemental Fig.…”

Section: Auxin Signaling Factors Are Required For De Novo Organ Regenmentioning

confidence: 76%

“…A study in leaf blades has shown that YUCCA1 (YUC1) and YUC4 appear to mediate synthesis of auxin in mesophyll cells (Chen et al, 2016). If this occurred in our system, auxin would also need to be transported to cells near the wound to induce de novo root organogenesis.…”

Section: Vasculature Proliferation and Endogenous Callus Formationmentioning

confidence: 88%

“…Supporting this idea, PIN-FORMED3 (PIN3) was expressed in the petiole vasculature whereas PIN4 was later restricted to the proliferating vascular region. In contrast, more delocalized auxin transport is involved in rooting of leaf blades Chen et al, 2016). As we Figure 8.…”

Section: Vasculature Proliferation and Endogenous Callus Formationmentioning

confidence: 99%

“…YUCCA-mediated auxin biosynthesis was shown to be ubiquitously enhanced in the leaf mesophyll and indirectly contribute to auxin accumulation near the excision site to trigger localized auxin signaling in the vasculature Chen et al, 2016). Formation of new roots involves formation of competent cells through auxin-induced expression of WOX11 transcription factor, which has been defined as a first step for cell fate transition during de novo organ regeneration .…”

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confidence: 99%

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Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

et al. 2017

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Body regeneration through formation of new organs is a major question in developmental biology. We investigated de novo root formation using whole leaves of Arabidopsis (Arabidopsis thaliana). Our results show that local cytokinin biosynthesis and auxin biosynthesis in the leaf blade followed by auxin long-distance transport to the petiole leads to proliferation of J0121-marked xylem-associated tissues and others through signaling of INDOLE-3-ACETIC ACID INDUCIBLE28 (IAA28), CRANE (IAA18), WOODEN LEG, and ARABIDOPSIS RESPONSE REGULATORS1 (ARR1), ARR10, and ARR12. Vasculature proliferation also involves the cell cycle regulator KIP-RELATED PROTEIN2 and ABERRANT LATERAL ROOT FORMATION4, resulting in a mass of cells with rooting competence that resembles callus formation. Endogenous callus formation precedes specification of postembryonic root founder cells, from which roots are initiated through the activity of SHORT-ROOT, PLETHORA1 (PLT1), and PLT2. Primordia initiation is blocked in shr plt1 plt2 mutant. Stem cell regulators SCHIZORIZA, JACKDAW, BLUEJAY, and SCARECROW also participate in root initiation and are required to pattern the new organ, as mutants show disorganized and reduced number of layers and tissue initials resulting in reduced rooting. Our work provides an organ regeneration model through de novo root formation, stating key stages and the primary pathways involved.Plants have striking regeneration capacities, and can produce new organs from postembryonic tissues (Hartmann et al., 2010;Chen et al., 2014;Liu et al., 2014) as well as reconstitute damaged organs upon wounding (Xu et al., 2006;Heyman et al., 2013; PerianezRodriguez et al., 2014;Melnyk et al., 2015;Efroni et al., 2016). Intriguingly, root regeneration upon stem cell damage recruits embryonic pathways (Hayashi et al., 2006;Efroni et al., 2016), whereas in contrast, postembryonic formation of whole new organs, such as lateral roots, appears to use specific postembryonic pathways (Lavenus et al., 2013).Cross talk between auxin and cytokinin signaling is required for many aspects of plant development and regeneration (El-Showk et al., 2013), although how their synergistic interaction is implemented at the molecular level has not been clarified (Skoog and Miller, 1957;Chandler and Werr, 2015). Exogenous in vitro supplementation of these two hormones results in continuous cell proliferation, to form a characteristic structure termed "callus". Callus emerges as a common regenerative mechanism for almost all plant organs through in vitro culture (Atta et al., 2009;Sugimoto et al., 2010). There is increasing evidence that callus formation requires hormone-mediated activation of a lateral and meristematic root development program in pericycle-like cells defined by expression of the J0121 marker 1 This work was supported by grants from Ministerio de Economía y Competitividad (MINECO) of Spain, the European Regional Development Fund (ERDF) and FP7 Funds of the European Commission, BFU2013-41160-P, BFU2016-80315-P, and PCIG11-GA-2012-322082 to M.A....

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Section: Auxin Signaling Factors Are Required For De Novo Organ Regenmentioning

confidence: 76%

Section: Vasculature Proliferation and Endogenous Callus Formationmentioning

confidence: 88%

Section: Vasculature Proliferation and Endogenous Callus Formationmentioning

confidence: 99%

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confidence: 99%

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Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

et al. 2017

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“…Auxin plays pivotal roles in the control of woundinduced root regeneration (Chen et al, 2016b), and it also promotes callus formation in tissue culture (Skoog and Miller, 1957). We examined transcriptional profiles of 13 auxin biosynthesis genes including YUCCA1-10 (YUC1-10) and TRP AMINOTRANSFERASE OF ARABIDOPSIS1 (TAA1) and found activation of only YUC5 and TAA1 after wounding (Fig.…”

Section: Wound Stress Does Not Modify Overall Auxin Biosynthesis or Rmentioning

confidence: 99%

Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes

et al. 2017

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Wounding is a primary trigger of organ regeneration, but how wound stress reactivates cell proliferation and promotes cellular reprogramming remains elusive. In this study, we combined transcriptome analysis with quantitative hormonal analysis to investigate how wounding induces callus formation in Arabidopsis (Arabidopsis thaliana). Our time course RNA-seq analysis revealed that wounding induces dynamic transcriptional changes, starting from rapid stress responses followed by the activation of metabolic processes and protein synthesis and subsequent activation of cell cycle regulators. Gene ontology analyses further uncovered that wounding modifies the expression of hormone biosynthesis and response genes, and quantitative analysis of endogenous plant hormones revealed accumulation of cytokinin prior to callus formation. Mutants defective in cytokinin synthesis and signaling display reduced efficiency in callus formation, indicating that de novo synthesis of cytokinin is critical for wound-induced callus formation. We further demonstrate that type-B ARABIDOPSIS RESPONSE REGULATOR-mediated cytokinin signaling regulates the expression of CYCLIN D3;1 (CYCD3;1) and that mutations in CYCD3;1 and its homologs CYCD3;2 and 3 cause defects in callus formation. In addition to these hormone-mediated changes, our transcriptome data uncovered that wounding activates multiple developmental regulators, and we found novel roles of ETHYLENE RESPONSE FACTOR 115 and PLETHORA3 (PLT3), PLT5, and PLT7 in callus generation. All together, these results provide novel mechanistic insights into how wounding reactivates cell proliferation during callus formation.

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Identification, analysis, and modeling of the YUCCA protein family genome‐wide in Coffea canephora

2021

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Auxin is involved in almost every aspect of plant growth and development, from embryogenesis to senescence. Indole-3-acetic acid (IAA) is the main known natural auxin that is synthesized by enzymes tryptophan aminotransferase of arabidopsis (TAA) and YUCCA (YUC) of the flavin-containing monooxygenases family (FMO) from one of the tryptophan-dependent pathways. Genome-wide identification and comprehensive analysis of the YUC-protein family have been conducted in Coffea canephora in the present study. A total of 10 members CcYUC gene family were identified in C. canephora. Phylogenetic analysis revealed that the CcYUC protein family is evolutionarily conserved, and they consist of four groups. In contrast, bioinformatic analysis predicted a hydrophobic transmembrane helix (TMH) for one CcYUC (YUC10) member only. Isoelectric point (pI), molecular mass (Ms), signal peptide, subcellular localization, and phosphorylation sites were predicted for CcYUC proteins.YUC enzymes require the prosthetic group flavin adenine dinucleotide (FAD) and the cofactor nicotinamide adenine dinucleotide phosphate (NADPH) for their enzymatic activity. Therefore, we include the molecular docking for CcYUC2-FAD-NADPH-IPyA and yucasin, which is a specific inhibitor for YUC activity. The docking results showed FAD and NADPH binding at the big and small domain sites, respectively, in CcYUC2. IPyA binds very close to FAD along the big domain, and yucasin competes for the same site as IPA, blocking IAA production. Furthermore, in silico point mutations affect the stability of the CcYUC2-4 proteins.

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YUCCA-mediated auxin biogenesis is required for cell fate transition occurring duringde novoroot organogenesis in Arabidopsis

Abstract: HighlightYUCCA family genes act in response to multiple signals in leaf explants and contribute to de novo auxin biogenesis for fate transition of regeneration-competent cells.

Cited by 168 publications

References 32 publications

Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

Regulation of Hormonal Control, Cell Reprogramming, and Patterning during De Novo Root Organogenesis

Wounding Triggers Callus Formation via Dynamic Hormonal and Transcriptional Changes

Identification, analysis, and modeling of the YUCCA protein family genome‐wide in Coffea canephora

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