γ-Irradiated gelatin and polyvinyl alcohol films as artificial articular cartilage

Abstract

Both gelatin and polyvinyl alcohol polymers have good biocompatibility to be used in biomedical applications. Cell adhesion and growth activity of gelatin have made it unique in artificial cartilage preparation. Two separate solutions of them are prepared by mixing 10% (w/v) of each one in water. These two solutions are then mixed at a temperature range of 70–90°C, and a pH value of 2 is maintained and finally it is cast into films. The dried films are gamma irradiated (using 60Co) at different doses. Fourier transform infrared analysis of the films shows the presence of amide linkages formed in them. It is observed from thermogravimetric analysis that 2.6% of non-irradiated and 20% of irradiated samples retain after heating them up to 600°C. Mechanical properties of the irradiated films show better properties than that of non-irradiated one. The irradiated films show a decrease in water uptake than that of the non-irradiated films. Scanning electron microscopy does not show any significant difference in morphology of the films with increasing radiation doses.

Gelatin-Polyvinyl Alcohol Film for Tissue Engineering: A Concise Review

“…Literature search demonstrated a various ratio of gelatin to PVA that has been reported in the selected articles. Al-Mamun et al [52] and Ebnalwaled et al [55] reported a 1:1 ratio of gelatin to PVA. In contrast, Basak et al [53] and Chaibi et al [50] used fixed ratios, which are 1:2 and 29:43, respectively.…”

“…As for gelatin, the final concentrations used by Chaibi et al [50] and Ismaiel et al [51] are almost similar, which are 0.05 and 0.048 g/mL, respectively. In contrast, Al-Mamun et al [52] used a higher concentration of 0.11 g/mL. Another study performed by Basak et al [53] described 5% of gelatin, however, Jain et al [54] used three different percentages of gelatin that are 1%, 2% and 3%.…”

“…Another study performed by Basak et al [53] described 5% of gelatin, however, Jain et al [54] used three different percentages of gelatin that are 1%, 2% and 3%. The PVA concentration in Al-Mamun et al [52] and Ismaiel [51] was 0.11 and 0.032 g/mL, respectively for their blended gelatin-PVA. Furthermore, Chaibi [50] scrutinised that PVA has been used around 0.2% by weight/volume.…”

“…Basak et al [53] and Chaibi et al [50] used glutaraldehyde as a crosslinker while Al-Mamun et al [52], Ismaiel et al [51], Ebnalwaled et al [55] and Khan et al [56] reported the use of gamma-irradiation as a method of crosslinking. While there are some studies that did not use any crosslinkers in their fabrication methods.…”

Biomedyczne właściwości chitozanu – zastosowanie w inżynierii tkankowej Biomedical properties of chitosan: Application in tissue engineering

“…W ciągu ostatnich dwóch dekad wzrosło zapotrzebowanie na naturalne materiały bioaktywne, gdyż nie wykazują toksyczności i są bezpieczniejsze w użytkowaniu niż polimery syntetyczne. Dużym zainteresowaniem w naprawie i regeneracji uszkodzonej tkanki cieszą się: chitozan, kolagen, żelatyna, alginian, fibroina, hydroksyapatyt czy kwas hialuronowy [12]. Ze względu na unikalne właściwości i dostępność chitozan był szeroko badany jako potencjalny materiał bioaktywny i stał się obiecującym materiałem w inżynierii tkankowej [10].…”