IAA and ACC deaminase producing-bacteria isolated from the rhizosphere of pineapple plants grown under different abiotic and biotic stresses

Ratnaningsih, Hanim  R.; Noviana, Zahra; Dewi, Tirta Kumala; Loekito, Supriyono; Wiyono, Suryo; Gafur, Abdul; Antonius, Sarjiya

doi:10.1016/j.heliyon.2023.e16306

Cited by 25 publications

(4 citation statements)

References 81 publications

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“…A subset of endophytes from each category exhibited these traits, indicating their potential to promote plant growth. This is supported by numerous previously published studies [ 49 , 50 , 51 , 52 , 53 , 54 ].…”

Section: Discussionsupporting

confidence: 88%

Isolation and Characterization of Bacterial Endophytes from Small Nodules of Field-Grown Peanut

Hossain,

Frith,

Bhattacharyya

et al. 2023

Microorganisms

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It is evident that legume root nodules can accommodate rhizobial and non-rhizobial bacterial endophytes. Our recent nodule microbiome study in peanuts described that small nodules can harbor diverse bacterial endophytes. To understand their functional role, we isolated 87 indigenous endophytes from small nodules of field-grown peanut roots and characterized them at molecular, biochemical, and physiological levels. The amplified 16S rRNA genes and phylogenetic analysis of these isolates revealed a wide variety of microorganisms related to the genera Bacillus, Burkholderia, Enterobacter, Herbaspirillum, Mistsuaria, Pantoea, Pseudomonas, and Rhizobia. It was observed that 37% (100% identity) and 56% (>99% identity) of the isolates matched with the amplified sequence variants (ASVs) from our previous microbiome study. All of these isolates were tested for stress tolerance (high temperature, salinity, acidic pH) and phosphate (P) solubilization along with ammonia (NH3), indole-3-acetic acid (IAA), 1-aminocyclopropane-1-carboxylate deaminase (ACCD), and siderophore production. The majority (78%) of the isolates were found to be halotolerant, thermotolerant, and acidophilic, and a few of them showed a significant positive response to the production of IAA, NH3, siderophore, ACCD, and P-solubilization. To evaluate the plant growth promotion (PGP) activity, plant and nodulation assays were performed in the growth chamber conditions for the selected isolates from both the non-rhizobial and rhizobial groups. However, these isolates appeared to be non-nodulating in the tested conditions. Nonetheless, the isolates 2 (Pantoea), 17 (Burkholderia), 21 (Herbaspirillum), 33o (Pseudomonas), and 77 (Rhizobium sp.) showed significant PGP activity in terms of biomass production. Our findings indicate that these isolates have potential for future biotechnological applications through the development of biologicals for sustainable crop improvement.

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Section: Discussionsupporting

confidence: 88%

Isolation and Characterization of Bacterial Endophytes from Small Nodules of Field-Grown Peanut

Hossain,

Frith,

Bhattacharyya

et al. 2023

Microorganisms

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“…Qualitative and quantitative determination of IAA production by B. subtilis PE7 Several studies reported that Bacillus species produced different types of phytohormones including IAA to regulate plant growth (Cherif-Silini et al, 2016;Ratnaningsih et al, 2023). As shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Growth Enhancement of Tomato by a Plant Growth Promoting Bacterium, Bacillus subtilis PE7

Han,

Kim,

Maung

et al. 2023

Korean J. Soil. Sci. Fert.

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Bacillus species are well recognized as effective bio-stimulants to enhance crop quality and productivity. Our study demonstrates the effect of a plant growth promoting bacterium, Bacillus subtilis PE7 on tomato growth. In vitro screening assay for plant growth promoting traits indicated that the indole-3-acetic acid (IAA) production of B. subtilis PE7 in terms of increasing intensity of pink color with the increasing concentration of ⳑ-tryptophan supplemented in tryptone soy broth (TSB) medium. The supplementation of ⳑ-tryptophan (1 g L -1 ) in the TSB medium resulted in the maximum IAA production of strain PE7 with 25.59 µg mL -1 at 6 days after incubation. Moreover, the highest ammonia production of strain PE7 with 21.22 µg mL -1 was observed at an incubation period of 72 h. The results of our pot experiment revealed that treatment with the strain PE7 culture (inoculated in fertilizer-based medium) induced a significant growth promotion of tomato plants resulting in higher values of leaf number, length, fresh and dry weights of shoot as compared to the control and fertilizer treatment. Therefore, B. subtilis PE7 could be considered as an effective candidate for the development of eco-friendly commercial fertilizer in crop production.

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“…The microorganism with this enzyme activity in the rhizosphere reduces plant ethylene levels, inhibits its detrimental effects, and protects against biotic and abiotic stresses ( Glick, 2014 ). Several plant growth-promoting bacteria have been isolated from the rhizosphere of pineapple from various stress-inducing habitats ( Ratnaningsih et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

IAA-producing plant growth promoting rhizobacteria from Ceanothus velutinus enhance cutting propagation efficiency and Arabidopsis biomass

Ganesh,

Hewitt,

Devkota

et al. 2024

Front. Plant Sci.

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Climate-induced drought impacts plant growth and development. Recurring droughts increase the demand for water for food production and landscaping. Native plants in the Intermountain West region of the US are of keen interest in low water use landscaping as they are acclimatized to dry and cold environments. These native plants do very well at their native locations but are difficult to propagate in landscape. One of the possible reasons is the lack of associated microbiome in the landscaping. Microbiome in the soil contributes to soil health and impacts plant growth and development. Here, we used the bulk soil from the native plant Ceanothus velutinus (snowbrush ceanothus) as inoculant to enhance its propagation. Snowbrush ceanothus is an ornamental plant for low-water landscaping that is hard to propagate asexually. Using 50% native bulk soil as inoculant in the potting mix significantly improved the survival rate of the cuttings compared to no-treated cuttings. Twenty-four plant growth-promoting rhizobacteria (PGPR) producing indole acetic acid (IAA) were isolated from the rhizosphere and roots of the survived snowbrush. Seventeen isolates had more than 10µg/mL of IAA were shortlisted and tested for seven different plant growth-promoting (PGP) traits; 76% showed nitrogen-fixing ability on Norris Glucose Nitrogen free media,70% showed phosphate solubilization activity, 76% showed siderophore production, 36% showed protease activity, 94% showed ACC deaminase activity on DF-ACC media, 76% produced catalase and all of isolates produced ammonia. Eight of seventeen isolates, CK-6, CK-22, CK-41, CK-44, CK-47, CK-50, CK-53, and CK-55, showed an increase in shoot biomass in Arabidopsis thaliana. Seven out of eight isolates were identified as Pseudomonas, except CK-55, identified as Sphingobium based on 16S rRNA gene sequencing. The shortlisted isolates are being tested on different grain and vegetable crops to mitigate drought stress and promote plant growth.

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IAA and ACC deaminase producing-bacteria isolated from the rhizosphere of pineapple plants grown under different abiotic and biotic stresses

Cited by 25 publications

References 81 publications

Isolation and Characterization of Bacterial Endophytes from Small Nodules of Field-Grown Peanut

Isolation and Characterization of Bacterial Endophytes from Small Nodules of Field-Grown Peanut

Growth Enhancement of Tomato by a Plant Growth Promoting Bacterium, Bacillus subtilis PE7

IAA-producing plant growth promoting rhizobacteria from Ceanothus velutinus enhance cutting propagation efficiency and Arabidopsis biomass

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