IBR5 Regulates Leaf Serrations Development via Modulation of the Expression of PIN1

Kong, Xiuzhen; Huang, Guoqiang; Xiong, Yali; Zhao, Chunyan; Wang, Jun; Song, Xiaoyun; Giri, Jitender; Zuo, Kaijing

doi:10.3390/ijms20184429

Cited by 11 publications

(8 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The methods for GUS staining of ProOsARF6/17:GUS were modified from a previously published protocol (Kong et al, 2019). Briefly, lamina joints of flag leaf at different stages were incubated in GUS staining solution (50-mM NaPO 4 buffer, pH 7.0, 10-mg mL À1 X-Gluc, and 0.02% (v/v) Triton X-100), at 37 C in the dark for 12 h, and then washed with 70% (v/v) ethanol for 72 h twice until transparent.…”

Section: Gus Staining Procedures and Cell Wall Labelingmentioning

confidence: 99%

AUXIN RESPONSE FACTORS 6 and 17 control the flag leaf angle in rice by regulating secondary cell wall biosynthesis of lamina joints

et al. 2021

Self Cite

View full text Add to dashboard Cite

Flag leaf angle impacts the photosynthetic capacity of densely grown plants and is thus an important agronomic breeding trait for crop architecture and yield. The hormone auxin plays a key role in regulating this trait, yet the underlying molecular and cellular mechanisms remain unclear. Here, we report that two rice (Oryza sativa) auxin response factors (ARFs), OsARF6 and OsARF17, which are highly expressed in lamina joint tissues, control flag leaf angle in response to auxin. Loss-of-function double osarf6 osarf17 mutants displayed reduced secondary cell wall levels of lamina joint sclerenchymatous cells (Sc), resulting in an exaggerated flag leaf angle and decreased grain yield under dense planting conditions. Mechanical measurements indicated that the mutant lamina joint tissues were too weak to support the weight of the flag leaf blade, resembling the phenotype of the rice increased leaf angle1 (ila1) mutant. We demonstrate that OsARF6 and OsARF17 directly bind to the ILA1 promoter independently and synergistically to activate its expression. In addition, auxin-induced ILA1 expression was dependent on OsARF6 and OsARF17. Collectively, our study reveals a mechanism that integrates auxin signaling with secondary cell wall composition to determine flag leaf angle, providing breeding targets in rice, and potentially other cereals, for this key trait.

show abstract

Section: Gus Staining Procedures and Cell Wall Labelingmentioning

confidence: 99%

AUXIN RESPONSE FACTORS 6 and 17 control the flag leaf angle in rice by regulating secondary cell wall biosynthesis of lamina joints

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…The optimal expression of the genes is necessary for continuous growth and development. The miRNAs mainly contribute to the regulation of gene expression [ 45 , 195 ]. In leaf serration, the boundary controlling gene CUC1 , CUC2 , and CUC3 is required for the maintenance of the shoot apical meristem [ 45 , 195 ].…”

Section: Leaf Margins Serrationsmentioning

confidence: 99%

“…The miRNAs mainly contribute to the regulation of gene expression [45,195]. In leaf serration, the boundary controlling gene CUC1, CUC2, and CUC3 is required for the maintenance of the shoot apical meristem [45,195]. Furthermore, reports indicate that CUC2 has a very important role in leaf serration.…”

Section: Leaf Margins Serrationsmentioning

confidence: 99%

Molecular and Hormonal Regulation of Leaf Morphogenesis in Arabidopsis

Ali

Khan

Xie

2020

IJMS

View full text Add to dashboard Cite

Shoot apical meristems (SAM) are tissues that function as a site of continuous organogenesis, which indicates that a small pool of pluripotent stem cells replenishes into lateral organs. The coordination of intercellular and intracellular networks is essential for maintaining SAM structure and size and also leads to patterning and formation of lateral organs. Leaves initiate from the flanks of SAM and then develop into a flattened structure with variable sizes and forms. This process is mainly regulated by the transcriptional regulators and mechanical properties that modulate leaf development. Leaf initiation along with proper orientation is necessary for photosynthesis and thus vital for plant survival. Leaf development is controlled by different components such as hormones, transcription factors, miRNAs, small peptides, and epigenetic marks. Moreover, the adaxial/abaxial cell fate, lamina growth, and shape of margins are determined by certain regulatory mechanisms. The over-expression and repression of various factors responsible for leaf initiation, development, and shape have been previously studied in several mutants. However, in this review, we collectively discuss how these factors modulate leaf development in the context of leaf initiation, polarity establishment, leaf flattening and shape.

show abstract

“…Arabidopsis typically has simple and small serrated leaves and is thus the ideal plant to study leaf shape and margin formation. Previous studies have shown that leaf serration formation depends on the guidance of auxin maxima at their tips ( Kawamura et al., 2010 ), and many proteins can regulate the leaf serrations of Arabidopsis by direct or indirect auxin process ( Kong et al., 2019 ). Auxin biosynthesis-related proteins, YUCCA (YUC), indole-3-acetic acid carboxyl methyltransferase (IAMT1), AUXIN-RESISTANT 1 (AUX1), and PIN-FORMED 1 (PIN1) ( Hay et al., 2006 ; Wenzel et al., 2007 ; Kim et al., 2011 ; Kasprzewska et al., 2015 ) can regulate the auxin maxima directly in the leaf tips for Arabidopsis .…”

Section: Introductionmentioning

confidence: 99%

Understanding the molecular mechanism of leaf morphogenesis in vegetable crops conduces to breeding process

Hao

Cao²,

Wang³

et al. 2022

Front. Plant Sci.

View full text Add to dashboard Cite

Leaf morphology can affect the development and yield of plants by regulating plant architecture and photosynthesis. Several factors can determine the final leaf morphology, including the leaf complexity, size, shape, and margin type, which suggests that leaf morphogenesis is a complex regulation network. The formation of diverse leaf morphology is precisely controlled by gene regulation on translation and transcription levels. To further reveal this, more and more genome data has been published for different kinds of vegetable crops and advanced genotyping approaches have also been applied to identify the causal genes for the target traits. Therefore, the studies on the molecular regulation of leaf morphogenesis in vegetable crops have also been largely improved. This review will summarize the progress on identified genes or regulatory mechanisms of leaf morphogenesis and development in vegetable crops. These identified markers can be applied for further molecular-assisted selection (MAS) in vegetable crops. Overall, the review will contribute to understanding the leaf morphology of different crops from the perspective of molecular regulation and shortening the breeding cycle for vegetable crops.

show abstract

IBR5 Regulates Leaf Serrations Development via Modulation of the Expression of PIN1

Cited by 11 publications

References 49 publications

AUXIN RESPONSE FACTORS 6 and 17 control the flag leaf angle in rice by regulating secondary cell wall biosynthesis of lamina joints

AUXIN RESPONSE FACTORS 6 and 17 control the flag leaf angle in rice by regulating secondary cell wall biosynthesis of lamina joints

Molecular and Hormonal Regulation of Leaf Morphogenesis in Arabidopsis

Understanding the molecular mechanism of leaf morphogenesis in vegetable crops conduces to breeding process

Contact Info

Product

Resources

About